Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels
The efficiency of injectable biomaterials as minimally invasive therapeutics significantly relies on biomaterial's characteristics, such as stability, biodegradation rate, and interaction with the host tissue, which requires real‐time tracking of the biomaterials. Fluorescence imaging is consid...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2021-07-01
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| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202000113 |
| _version_ | 1818576820937687040 |
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| author | Fatemeh Nasrollahi Farzana Nazir Maryam Tavafoghi Vahid Hosseini Mohammad Ali Darabi David Paramelle Ali Khademhosseini Samad Ahadian |
| author_facet | Fatemeh Nasrollahi Farzana Nazir Maryam Tavafoghi Vahid Hosseini Mohammad Ali Darabi David Paramelle Ali Khademhosseini Samad Ahadian |
| author_sort | Fatemeh Nasrollahi |
| collection | DOAJ |
| description | The efficiency of injectable biomaterials as minimally invasive therapeutics significantly relies on biomaterial's characteristics, such as stability, biodegradation rate, and interaction with the host tissue, which requires real‐time tracking of the biomaterials. Fluorescence imaging is considered as a noninvasive technique for monitoring biomaterials; however, the commonly used fluorescent agents are often accompanied by photobleaching and toxicity. Herein, graphene quantum dots (GQDs) are introduced as a biocompatible and stable fluorophore for imaging and noninvasive monitoring of a physically cross‐linked injectable shear‐thinning biomaterial (STB) of gelatin–silicate nanoplatelets. Silicate nanoplatelets and GQDs serve as the physical cross‐linkers of gelatin making electrostatic interaction with gelatin chains. Different STB‐GQDs formulations are assessed in terms of fluorescence intensity, injectability, thermal stability, and cellular biocompatibility. STB‐GQDs with 0.06% GQDs, 6% solid material, and 50% silicate in the solid material show the strongest in vitro fluorescence and the highest thermal stability. In vivo monitoring of STB‐GQDs is also achieved through fluorescent imaging where incorporated GQDs exhibit a robust and stable signal, suggesting their promising applications in long‐term tracking of gelatin‐based STBs. |
| first_indexed | 2024-12-16T06:20:06Z |
| format | Article |
| id | doaj.art-6b95bbafec6d41588fbc85a5ec64dfa7 |
| institution | Directory Open Access Journal |
| issn | 2699-9307 |
| language | English |
| last_indexed | 2024-12-16T06:20:06Z |
| publishDate | 2021-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj.art-6b95bbafec6d41588fbc85a5ec64dfa72022-12-21T22:41:10ZengWiley-VCHAdvanced NanoBiomed Research2699-93072021-07-0117n/an/a10.1002/anbr.202000113Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning HydrogelsFatemeh Nasrollahi0Farzana Nazir1Maryam Tavafoghi2Vahid Hosseini3Mohammad Ali Darabi4David Paramelle5Ali Khademhosseini6Samad Ahadian7Department of Bioengineering University of California-Los Angeles Los Angeles CA 90095 USADepartment of Bioengineering University of California-Los Angeles Los Angeles CA 90095 USADepartment of Bioengineering University of California-Los Angeles Los Angeles CA 90095 USADepartment of Bioengineering University of California-Los Angeles Los Angeles CA 90095 USADepartment of Bioengineering University of California-Los Angeles Los Angeles CA 90095 USAInstitute of Materials Research and Engineering A*STAR (Agency for Science, Technology and Research) 2 Fusionopolis Way, Innovis #08-03 Singapore 138634 SingaporeTerasaki Institute for Biomedical Innovation (TIBI) Los Angeles CA 90024 USATerasaki Institute for Biomedical Innovation (TIBI) Los Angeles CA 90024 USAThe efficiency of injectable biomaterials as minimally invasive therapeutics significantly relies on biomaterial's characteristics, such as stability, biodegradation rate, and interaction with the host tissue, which requires real‐time tracking of the biomaterials. Fluorescence imaging is considered as a noninvasive technique for monitoring biomaterials; however, the commonly used fluorescent agents are often accompanied by photobleaching and toxicity. Herein, graphene quantum dots (GQDs) are introduced as a biocompatible and stable fluorophore for imaging and noninvasive monitoring of a physically cross‐linked injectable shear‐thinning biomaterial (STB) of gelatin–silicate nanoplatelets. Silicate nanoplatelets and GQDs serve as the physical cross‐linkers of gelatin making electrostatic interaction with gelatin chains. Different STB‐GQDs formulations are assessed in terms of fluorescence intensity, injectability, thermal stability, and cellular biocompatibility. STB‐GQDs with 0.06% GQDs, 6% solid material, and 50% silicate in the solid material show the strongest in vitro fluorescence and the highest thermal stability. In vivo monitoring of STB‐GQDs is also achieved through fluorescent imaging where incorporated GQDs exhibit a robust and stable signal, suggesting their promising applications in long‐term tracking of gelatin‐based STBs.https://doi.org/10.1002/anbr.202000113fluorescence imaginggraphene quantum dotsinjectable biomaterialsshear thinning |
| spellingShingle | Fatemeh Nasrollahi Farzana Nazir Maryam Tavafoghi Vahid Hosseini Mohammad Ali Darabi David Paramelle Ali Khademhosseini Samad Ahadian Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels Advanced NanoBiomed Research fluorescence imaging graphene quantum dots injectable biomaterials shear thinning |
| title | Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels |
| title_full | Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels |
| title_fullStr | Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels |
| title_full_unstemmed | Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels |
| title_short | Graphene Quantum Dots for Fluorescent Labeling of Gelatin‐Based Shear‐Thinning Hydrogels |
| title_sort | graphene quantum dots for fluorescent labeling of gelatin based shear thinning hydrogels |
| topic | fluorescence imaging graphene quantum dots injectable biomaterials shear thinning |
| url | https://doi.org/10.1002/anbr.202000113 |
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