First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins
Background: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345820300518 |
| _version_ | 1818870886543917056 |
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| author | Consuelo Borie Cristian Bravo Phillip Dettleff Nicolás Galarce Jessica Dorner Víctor Martínez |
| author_facet | Consuelo Borie Cristian Bravo Phillip Dettleff Nicolás Galarce Jessica Dorner Víctor Martínez |
| author_sort | Consuelo Borie |
| collection | DOAJ |
| description | Background: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains. Results: Genome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains. Conclusions: This work sequenced and characterized the complete genome of B. canis strain SCL, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent.How to cite: Borie C, Bravo C, Dettleff P, et al. First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins Electron J Biotechnol 2020;49. https://dx.doi.org/10.1016/j.ejbt.2020.10.002. |
| first_indexed | 2024-12-19T12:14:09Z |
| format | Article |
| id | doaj.art-6b96549b27a741fab4ab7f271a61d215 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-19T12:14:09Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-6b96549b27a741fab4ab7f271a61d2152022-12-21T20:22:04ZengElsevierElectronic Journal of Biotechnology0717-34582021-01-014914First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical originsConsuelo Borie0Cristian Bravo1Phillip Dettleff2Nicolás Galarce3Jessica Dorner4Víctor Martínez5Laboratorio de Microbiología Clínica Veterinaria, Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile. Av. Santa Rosa 11735, La Pintana, Santiago, ChileUnidad de Investigaciones en Biotecnología y Genómica Animal, Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, ChileUnidad de Investigaciones en Biotecnología y Genómica Animal, Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, ChileLaboratorio de Microbiología Clínica Veterinaria, Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile. Av. Santa Rosa 11735, La Pintana, Santiago, ChileUnidad de Investigaciones en Biotecnología y Genómica Animal, Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, ChileUnidad de Investigaciones en Biotecnología y Genómica Animal, Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, Chile; Corresponding author.Background: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains. Results: Genome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains. Conclusions: This work sequenced and characterized the complete genome of B. canis strain SCL, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent.How to cite: Borie C, Bravo C, Dettleff P, et al. First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins Electron J Biotechnol 2020;49. https://dx.doi.org/10.1016/j.ejbt.2020.10.002.http://www.sciencedirect.com/science/article/pii/S0717345820300518Brucella canisCanine brucellosisChileDogsGenomeSCL strain |
| spellingShingle | Consuelo Borie Cristian Bravo Phillip Dettleff Nicolás Galarce Jessica Dorner Víctor Martínez First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins Electronic Journal of Biotechnology Brucella canis Canine brucellosis Chile Dogs Genome SCL strain |
| title | First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins |
| title_full | First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins |
| title_fullStr | First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins |
| title_full_unstemmed | First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins |
| title_short | First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins |
| title_sort | first genome sequence of chilean brucella canis scl strain provides insights on the epidemiology and virulence factors explaining differences between geographical origins |
| topic | Brucella canis Canine brucellosis Chile Dogs Genome SCL strain |
| url | http://www.sciencedirect.com/science/article/pii/S0717345820300518 |
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