Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves
Background: Woodfordia fruticosa is used traditionally as antidysenteric compound for fodder animals. Stem and flower extracts are previously flourished for their antibacterial activity. Leaves are also equally important, which are preliminary screened for their bioactive components in present study...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2009-09-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-333-en.html |
| _version_ | 1818664348748349440 |
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| author | AD Chougale MV Padul Md Saiful Arfeen SL Kakad |
| author_facet | AD Chougale MV Padul Md Saiful Arfeen SL Kakad |
| author_sort | AD Chougale |
| collection | DOAJ |
| description | Background: Woodfordia fruticosa is used traditionally as antidysenteric compound for fodder animals. Stem and flower extracts are previously flourished for their antibacterial activity. Leaves are also equally important, which are preliminary screened for their bioactive components in present study. Objective: Evaluation of antibacterial activity of leaves of W. fruticosa and detection of their active compound. Methods: Different solvent i.e. petroleum ether, chloroform, diethyl ether and acetone extracts were studied against four bacterial strains using disc diffusion assay. Most potenz extract was chromatogram on preparative TLC. Further individual separated spots were back checked for their activity. Probable constituents of most active fraction were detected on GC-MS. Results: All four extracts exhibited antibacterial activity. The acetone extract showed highest inhibition against Bacillus subtilis NCIM 2921. The 80g and 120 g of acetone extract exhibit comparable antimicrobial activity against the four different bacteria studied. Amongst the four different spots on TLC chromatogram three of them had activity against B. subtilis NCIM 2921. The most active fraction was collected by preparative TLC and used for GC-MS, which exhibited two peaks. Conclusion: Extracts of petroleum ether, chloroform, diethyl ether and acetone were found to be effective against all the srains studied. The fraction of acetone extract, which is more active, constitutes two different compounds, in which both or one of them is responsible for antibacterial activity. |
| first_indexed | 2024-12-17T05:31:19Z |
| format | Article |
| id | doaj.art-6b971d3c5de742babc34749b86a4416d |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-17T05:31:19Z |
| publishDate | 2009-09-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-6b971d3c5de742babc34749b86a4416d2022-12-21T22:01:43ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582009-09-018317581Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. LeavesAD Chougale0MV Padul1Md Saiful Arfeen2SL Kakad3 Biochemistry Department, NACAS College, Pune University, Maharashtra-414001 Biochemistry Department, NACAS College, Pune University, Maharashtra-414001 Biotechnology Department, NACAS College, Pune University, Maharashtra-414001 Biochemistry Department, NACAS College, Pune University, Maharashtra-414001 Background: Woodfordia fruticosa is used traditionally as antidysenteric compound for fodder animals. Stem and flower extracts are previously flourished for their antibacterial activity. Leaves are also equally important, which are preliminary screened for their bioactive components in present study. Objective: Evaluation of antibacterial activity of leaves of W. fruticosa and detection of their active compound. Methods: Different solvent i.e. petroleum ether, chloroform, diethyl ether and acetone extracts were studied against four bacterial strains using disc diffusion assay. Most potenz extract was chromatogram on preparative TLC. Further individual separated spots were back checked for their activity. Probable constituents of most active fraction were detected on GC-MS. Results: All four extracts exhibited antibacterial activity. The acetone extract showed highest inhibition against Bacillus subtilis NCIM 2921. The 80g and 120 g of acetone extract exhibit comparable antimicrobial activity against the four different bacteria studied. Amongst the four different spots on TLC chromatogram three of them had activity against B. subtilis NCIM 2921. The most active fraction was collected by preparative TLC and used for GC-MS, which exhibited two peaks. Conclusion: Extracts of petroleum ether, chloroform, diethyl ether and acetone were found to be effective against all the srains studied. The fraction of acetone extract, which is more active, constitutes two different compounds, in which both or one of them is responsible for antibacterial activity.http://jmp.ir/article-1-333-en.htmlwoodfordia fruticosaantibacterial activitysolvent validationbioactive components |
| spellingShingle | AD Chougale MV Padul Md Saiful Arfeen SL Kakad Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves Journal of Medicinal Plants woodfordia fruticosa antibacterial activity solvent validation bioactive components |
| title | Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves |
| title_full | Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves |
| title_fullStr | Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves |
| title_full_unstemmed | Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves |
| title_short | Antibacterial Activity Directed Fractionation of Woodfordia fruticosa Kurz. Leaves |
| title_sort | antibacterial activity directed fractionation of woodfordia fruticosa kurz leaves |
| topic | woodfordia fruticosa antibacterial activity solvent validation bioactive components |
| url | http://jmp.ir/article-1-333-en.html |
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