Molecular markers. Amplified fragment length polymorphism
Amplified Fragment Length Polymorphism molecular markers (AFLPs) has been developed combining procedures of RFLPs and RAPDs molekular markers, i.e. the first step is restriction digestion of the genomic DNA that is followed by selective amplification of the restricted fragments. The advantage of the...
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| Format: | Article |
| Language: | English |
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Serbian Association of Plant Breedings and Seed Producers
2005-01-01
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| Series: | Selekcija i Semenarstvo |
| Subjects: | |
| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2005/0354-58810504069P.pdf |
| _version_ | 1811280477856727040 |
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| author | Pržulj Novo Perović Dragan |
| author_facet | Pržulj Novo Perović Dragan |
| author_sort | Pržulj Novo |
| collection | DOAJ |
| description | Amplified Fragment Length Polymorphism molecular markers (AFLPs) has been developed combining procedures of RFLPs and RAPDs molekular markers, i.e. the first step is restriction digestion of the genomic DNA that is followed by selective amplification of the restricted fragments. The advantage of the AFLP technique is that it allows rapid generation of a large number of reproducible markers. The reproducibility of AFLPs markers is assured by the use of restriction site-specific adapters and adapter-specific primers for PCR reaction. Only fragments containing the restriction site sequence plus the additional nucleotides will be amplified and the more selected nucleotides added on the primer sequence the fewer the number of fragments amplified by PCR. The amplified products are normally separated on a sequencing gel and visualized after exposure to X-ray film or by using fluorescent labeled primers. AFLP shave proven to be extremely proficient in revealing diversity at below the species level. A disadvantage of AFLP technique is that AFLPs are essentially a dominant marker system and not able to identify heterozygotes. |
| first_indexed | 2024-04-13T01:15:22Z |
| format | Article |
| id | doaj.art-6b98990b184143b59cae63a179b3f2a2 |
| institution | Directory Open Access Journal |
| issn | 0354-5881 2406-209X |
| language | English |
| last_indexed | 2024-04-13T01:15:22Z |
| publishDate | 2005-01-01 |
| publisher | Serbian Association of Plant Breedings and Seed Producers |
| record_format | Article |
| series | Selekcija i Semenarstvo |
| spelling | doaj.art-6b98990b184143b59cae63a179b3f2a22022-12-22T03:08:56ZengSerbian Association of Plant Breedings and Seed ProducersSelekcija i Semenarstvo0354-58812406-209X2005-01-01111-469790354-58810504069PMolecular markers. Amplified fragment length polymorphismPržulj Novo0Perović Dragan1Crop and Vegetable Scientific Institute, Novi Sad, SerbiaInstitute of Plant Genetics and Crop Plant Research, Department of Genebank, AGMOM, Gatersleben, GermanyAmplified Fragment Length Polymorphism molecular markers (AFLPs) has been developed combining procedures of RFLPs and RAPDs molekular markers, i.e. the first step is restriction digestion of the genomic DNA that is followed by selective amplification of the restricted fragments. The advantage of the AFLP technique is that it allows rapid generation of a large number of reproducible markers. The reproducibility of AFLPs markers is assured by the use of restriction site-specific adapters and adapter-specific primers for PCR reaction. Only fragments containing the restriction site sequence plus the additional nucleotides will be amplified and the more selected nucleotides added on the primer sequence the fewer the number of fragments amplified by PCR. The amplified products are normally separated on a sequencing gel and visualized after exposure to X-ray film or by using fluorescent labeled primers. AFLP shave proven to be extremely proficient in revealing diversity at below the species level. A disadvantage of AFLP technique is that AFLPs are essentially a dominant marker system and not able to identify heterozygotes.http://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2005/0354-58810504069P.pdfAFLPmolecular markersrestriction enzymesselective amplificationspecific adaptersspecific primers |
| spellingShingle | Pržulj Novo Perović Dragan Molecular markers. Amplified fragment length polymorphism Selekcija i Semenarstvo AFLP molecular markers restriction enzymes selective amplification specific adapters specific primers |
| title | Molecular markers. Amplified fragment length polymorphism |
| title_full | Molecular markers. Amplified fragment length polymorphism |
| title_fullStr | Molecular markers. Amplified fragment length polymorphism |
| title_full_unstemmed | Molecular markers. Amplified fragment length polymorphism |
| title_short | Molecular markers. Amplified fragment length polymorphism |
| title_sort | molecular markers amplified fragment length polymorphism |
| topic | AFLP molecular markers restriction enzymes selective amplification specific adapters specific primers |
| url | http://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2005/0354-58810504069P.pdf |
| work_keys_str_mv | AT przuljnovo molecularmarkersamplifiedfragmentlengthpolymorphism AT perovicdragan molecularmarkersamplifiedfragmentlengthpolymorphism |