The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals
<i>Background and Objectives: Candida albicans</i> causes various diseases ranging from superficial mycoses to life-threatening systemic infections often associated with biofilm formation, including mixed fungal–bacterial consortia. The biofilm matrix protects cells, making <i>Cand...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/58/12/1710 |
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| author | Alina Gatina Elena Trizna Alena Kolesnikova Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Airat Kayumov |
| author_facet | Alina Gatina Elena Trizna Alena Kolesnikova Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Airat Kayumov |
| author_sort | Alina Gatina |
| collection | DOAJ |
| description | <i>Background and Objectives: Candida albicans</i> causes various diseases ranging from superficial mycoses to life-threatening systemic infections often associated with biofilm formation, including mixed fungal–bacterial consortia. The biofilm matrix protects cells, making <i>Candida</i> extremely resistant to treatment. Here, we show that the bovhyaluronidase azoximer (Longidaza<sup>®</sup>) in vitro destroys the biofilm formed by either <i>C. albicans</i> alone or mixed with bacteria, this way decreasing the concentrations of antimicrobials required for the pathogen’s eradication. <i>Materials and Methods:</i> Bovhyaluronidase azoximer, Longidaza® was obtained from NPO Petrovax Pharm Ltd., Moscow, Russia as lyophilized powder. The antifungal activity was assessed by microdilution assay and CFUs counting. Antibiofilm activity was evaluated via biofilms staining and scanning electron microscopy. <i>Results:</i> Thus, treatment with Longidaza<sup>®</sup> reduced the biofilm biomass of nine <i>C. albicans</i> clinical isolates by 30–60%, while mixed biofilms of <i>C. albicans</i> with various bacteria were destroyed by 30–40%. Furthermore, the concentration of fluconazole required to achieve a similar reduction of the residual respiratory activity of detached cell clumps of four <i>C. albicans</i> isolates has been reduced four-fold when combined with Longidaza<sup>®</sup>. While in the biofilm, two of four isolates became significantly more susceptible to fluconazole in combination with Longidaza<sup>®</sup>. <i>Conclusion:</i> Taken together, our data indicate that Longidaza<sup>®</sup> is capable of suppression of tissues and artificial surfaces biofouling by <i>C. albicans</i> biofilms, as well as facilitating drug penetration into the cell clumps, this way decreasing the effective MIC of antifungals. |
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| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T16:08:07Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj.art-6b992836243d4bf59ab37f2b9f9f33f72023-11-24T16:31:20ZengMDPI AGMedicina1010-660X1648-91442022-11-015812171010.3390/medicina58121710The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of AntifungalsAlina Gatina0Elena Trizna1Alena Kolesnikova2Diana Baidamshina3Anna Gorshkova4Valentin Drucker5Mikhail Bogachev6Airat Kayumov7Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaLimnological Institute of the Siberian Branch of the Russian Academy of Sciences, 664000 Irkutsk, RussiaLimnological Institute of the Siberian Branch of the Russian Academy of Sciences, 664000 Irkutsk, RussiaBiomedical Engineering Research Centre, St. Petersburg Electrotechnical University, 197022 St. Petersburg, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia<i>Background and Objectives: Candida albicans</i> causes various diseases ranging from superficial mycoses to life-threatening systemic infections often associated with biofilm formation, including mixed fungal–bacterial consortia. The biofilm matrix protects cells, making <i>Candida</i> extremely resistant to treatment. Here, we show that the bovhyaluronidase azoximer (Longidaza<sup>®</sup>) in vitro destroys the biofilm formed by either <i>C. albicans</i> alone or mixed with bacteria, this way decreasing the concentrations of antimicrobials required for the pathogen’s eradication. <i>Materials and Methods:</i> Bovhyaluronidase azoximer, Longidaza® was obtained from NPO Petrovax Pharm Ltd., Moscow, Russia as lyophilized powder. The antifungal activity was assessed by microdilution assay and CFUs counting. Antibiofilm activity was evaluated via biofilms staining and scanning electron microscopy. <i>Results:</i> Thus, treatment with Longidaza<sup>®</sup> reduced the biofilm biomass of nine <i>C. albicans</i> clinical isolates by 30–60%, while mixed biofilms of <i>C. albicans</i> with various bacteria were destroyed by 30–40%. Furthermore, the concentration of fluconazole required to achieve a similar reduction of the residual respiratory activity of detached cell clumps of four <i>C. albicans</i> isolates has been reduced four-fold when combined with Longidaza<sup>®</sup>. While in the biofilm, two of four isolates became significantly more susceptible to fluconazole in combination with Longidaza<sup>®</sup>. <i>Conclusion:</i> Taken together, our data indicate that Longidaza<sup>®</sup> is capable of suppression of tissues and artificial surfaces biofouling by <i>C. albicans</i> biofilms, as well as facilitating drug penetration into the cell clumps, this way decreasing the effective MIC of antifungals.https://www.mdpi.com/1648-9144/58/12/1710<i>Candida albicans</i>biofilmsenzymatic destruction of the biofilmbovhyaluronidase azoximer (Longidaza<sup>®</sup>) |
| spellingShingle | Alina Gatina Elena Trizna Alena Kolesnikova Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Airat Kayumov The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals Medicina <i>Candida albicans</i> biofilms enzymatic destruction of the biofilm bovhyaluronidase azoximer (Longidaza<sup>®</sup>) |
| title | The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals |
| title_full | The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals |
| title_fullStr | The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals |
| title_full_unstemmed | The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals |
| title_short | The Bovhyaluronidase Azoximer (Longidaza<sup>®</sup>) Disrupts <i>Candida albicans</i> and <i>Candida albicans</i>-Bacterial Mixed Biofilms and Increases the Efficacy of Antifungals |
| title_sort | bovhyaluronidase azoximer longidaza sup r sup disrupts i candida albicans i and i candida albicans i bacterial mixed biofilms and increases the efficacy of antifungals |
| topic | <i>Candida albicans</i> biofilms enzymatic destruction of the biofilm bovhyaluronidase azoximer (Longidaza<sup>®</sup>) |
| url | https://www.mdpi.com/1648-9144/58/12/1710 |
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