Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression
Background Cervical cancer is a major cause of morbidity and mortality in women worldwide. The underlying mechanisms of its progression are not well understood. In this study, we investigated the role of lymphoid-specific helicase (HELLS) in cervical cancer. Methods We measured HELLS expression in c...
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| Format: | Article |
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PeerJ Inc.
2023-11-01
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| Online Access: | https://peerj.com/articles/16451.pdf |
| _version_ | 1827629698653356032 |
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| author | Weiwei Tie Fenfen Ge |
| author_facet | Weiwei Tie Fenfen Ge |
| author_sort | Weiwei Tie |
| collection | DOAJ |
| description | Background Cervical cancer is a major cause of morbidity and mortality in women worldwide. The underlying mechanisms of its progression are not well understood. In this study, we investigated the role of lymphoid-specific helicase (HELLS) in cervical cancer. Methods We measured HELLS expression in cervical cancer and assessed its function using gain- and loss-of-function experiments. Cell viability was measured using the Cell Counting Kit-8 (CCK8 ) assay, and cell proliferation was analyzed using colony formation and EdU assays. Results We found that HELLS was significantly increased in cervical cancer and that its overexpression promoted cell viability (P < 0.01) and colony formation (P < 0.001). In contrast, si-HELLS suppressed these effects. Moreover, HELLS overexpression inhibited cell death induced by the ferroptosis inducer erastin (P < 0.01). Mechanistically, we found that HELLS promoted cervical cancer proliferation by regulating nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated ferroptosis. Conclusion Our data suggest that HELLS promotes cervical cancer proliferation by inhibiting Nrf2 expression. Therefore, HELLS knockdown may be an effective treatment for cervical cancer. |
| first_indexed | 2024-03-09T13:56:07Z |
| format | Article |
| id | doaj.art-6b99897d83684569a3d28c24e9015369 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T13:56:07Z |
| publishDate | 2023-11-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-6b99897d83684569a3d28c24e90153692023-11-30T15:05:12ZengPeerJ Inc.PeerJ2167-83592023-11-0111e1645110.7717/peerj.16451Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expressionWeiwei Tie0Fenfen Ge1Department of Gynecology, Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Gynecology, Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, ChinaBackground Cervical cancer is a major cause of morbidity and mortality in women worldwide. The underlying mechanisms of its progression are not well understood. In this study, we investigated the role of lymphoid-specific helicase (HELLS) in cervical cancer. Methods We measured HELLS expression in cervical cancer and assessed its function using gain- and loss-of-function experiments. Cell viability was measured using the Cell Counting Kit-8 (CCK8 ) assay, and cell proliferation was analyzed using colony formation and EdU assays. Results We found that HELLS was significantly increased in cervical cancer and that its overexpression promoted cell viability (P < 0.01) and colony formation (P < 0.001). In contrast, si-HELLS suppressed these effects. Moreover, HELLS overexpression inhibited cell death induced by the ferroptosis inducer erastin (P < 0.01). Mechanistically, we found that HELLS promoted cervical cancer proliferation by regulating nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated ferroptosis. Conclusion Our data suggest that HELLS promotes cervical cancer proliferation by inhibiting Nrf2 expression. Therefore, HELLS knockdown may be an effective treatment for cervical cancer.https://peerj.com/articles/16451.pdfCervical cancerFerroptosisLymphoid-specific helicaseNuclear factor erythroid 2-related factor 2 |
| spellingShingle | Weiwei Tie Fenfen Ge Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression PeerJ Cervical cancer Ferroptosis Lymphoid-specific helicase Nuclear factor erythroid 2-related factor 2 |
| title | Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression |
| title_full | Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression |
| title_fullStr | Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression |
| title_full_unstemmed | Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression |
| title_short | Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression |
| title_sort | lymphoid specific helicase inhibits cervical cancer cells ferroptosis by promoting nrf2 expression |
| topic | Cervical cancer Ferroptosis Lymphoid-specific helicase Nuclear factor erythroid 2-related factor 2 |
| url | https://peerj.com/articles/16451.pdf |
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