Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study
Ex-vivo freshly surgical removed pancreatic ductal adenocarcinoma (PDAC) specimens were assessed using pCLE and then processed for paraffin embeding and histopathological diagnostic in an endeavour to find putative image analysis algorithms that might recognise adenocarcinoma. Methods: Twelve patien...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/11/923 |
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| author | Bogdan Silviu Ungureanu Daniel Pirici Simona Olimpia Dima Irinel Popescu Gheorghe Hundorfean Valeriu Surlin Adrian Saftoiu |
| author_facet | Bogdan Silviu Ungureanu Daniel Pirici Simona Olimpia Dima Irinel Popescu Gheorghe Hundorfean Valeriu Surlin Adrian Saftoiu |
| author_sort | Bogdan Silviu Ungureanu |
| collection | DOAJ |
| description | Ex-vivo freshly surgical removed pancreatic ductal adenocarcinoma (PDAC) specimens were assessed using pCLE and then processed for paraffin embeding and histopathological diagnostic in an endeavour to find putative image analysis algorithms that might recognise adenocarcinoma. Methods: Twelve patients diagnosed with PDAC on endoscopic ultrasound and FNA confirmation underwent surgery. Removed samples were sprayed with acriflavine as contrast agent, underwent pCLE with an experimental probe and compared with previous recordings of normal pancreatic tissue. Subsequently, all samples were subjected to cross-sectional histopathology, including surgical resection margins for controls. pCLE records, as well as corespondant cytokeratin-targeted immunohistochemistry images were processed using the same morphological classifiers in the Image ProPlus AMS image analysis software. Specific morphometric classifiers were automatically generated on all images: Area, Hole Area (HA), Perimeter, Roundness, Integrated Optical Density (IOD), Fractal Dimension (FD), Ferret max (Fmax), Ferret mean (Fmean), Heterogeneity and Clumpiness. Results: After histopathological confirmation of adenocarcinoma areas, we have found that the same morphological classifiers could clearly differentiate between tumor and non-tumor areas on both pathology and correspondand pCLE (area, roundness, IOD, ferret and heterogeneity (<i>p</i> < 0.001), perimeter and hole area (<i>p</i> < 0.05). Conclusions: This pilot study proves that classical morphometrical classifiers can clearly differentiate adenocarcimoma on pCLE data, and the implementation in a live image-analysis algorithm might help in improving the specificity of pCLE in vivo diagnostic. |
| first_indexed | 2024-03-10T14:58:51Z |
| format | Article |
| id | doaj.art-6b9d36838f0a4d94983a162b9d62a916 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T14:58:51Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-6b9d36838f0a4d94983a162b9d62a9162023-11-20T20:22:11ZengMDPI AGDiagnostics2075-44182020-11-01101192310.3390/diagnostics10110923Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot StudyBogdan Silviu Ungureanu0Daniel Pirici1Simona Olimpia Dima2Irinel Popescu3Gheorghe Hundorfean4Valeriu Surlin5Adrian Saftoiu6Gastroenterology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaHistology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaSurgical Department, Fundeni Clinical Institute, 925200 Bucharest, RomaniaSurgical Department, Fundeni Clinical Institute, 925200 Bucharest, RomaniaMedical Clinic 1, Department of Medicine, University Hospital Erlangen, University Erlangen-Nuremberg, 91052 Erlangen, GermanySurgical Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaGastroenterology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaEx-vivo freshly surgical removed pancreatic ductal adenocarcinoma (PDAC) specimens were assessed using pCLE and then processed for paraffin embeding and histopathological diagnostic in an endeavour to find putative image analysis algorithms that might recognise adenocarcinoma. Methods: Twelve patients diagnosed with PDAC on endoscopic ultrasound and FNA confirmation underwent surgery. Removed samples were sprayed with acriflavine as contrast agent, underwent pCLE with an experimental probe and compared with previous recordings of normal pancreatic tissue. Subsequently, all samples were subjected to cross-sectional histopathology, including surgical resection margins for controls. pCLE records, as well as corespondant cytokeratin-targeted immunohistochemistry images were processed using the same morphological classifiers in the Image ProPlus AMS image analysis software. Specific morphometric classifiers were automatically generated on all images: Area, Hole Area (HA), Perimeter, Roundness, Integrated Optical Density (IOD), Fractal Dimension (FD), Ferret max (Fmax), Ferret mean (Fmean), Heterogeneity and Clumpiness. Results: After histopathological confirmation of adenocarcinoma areas, we have found that the same morphological classifiers could clearly differentiate between tumor and non-tumor areas on both pathology and correspondand pCLE (area, roundness, IOD, ferret and heterogeneity (<i>p</i> < 0.001), perimeter and hole area (<i>p</i> < 0.05). Conclusions: This pilot study proves that classical morphometrical classifiers can clearly differentiate adenocarcimoma on pCLE data, and the implementation in a live image-analysis algorithm might help in improving the specificity of pCLE in vivo diagnostic.https://www.mdpi.com/2075-4418/10/11/923pancreatic adenocarcinomaconfocal laser endomicroscopyendoscopic ultrasound |
| spellingShingle | Bogdan Silviu Ungureanu Daniel Pirici Simona Olimpia Dima Irinel Popescu Gheorghe Hundorfean Valeriu Surlin Adrian Saftoiu Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study Diagnostics pancreatic adenocarcinoma confocal laser endomicroscopy endoscopic ultrasound |
| title | Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study |
| title_full | Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study |
| title_fullStr | Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study |
| title_full_unstemmed | Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study |
| title_short | Morphometric Assessment of Confocal Laser Endomicroscopy for Pancreatic Ductal Adenocarcinoma, an Ex-Vivo Pilot Study |
| title_sort | morphometric assessment of confocal laser endomicroscopy for pancreatic ductal adenocarcinoma an ex vivo pilot study |
| topic | pancreatic adenocarcinoma confocal laser endomicroscopy endoscopic ultrasound |
| url | https://www.mdpi.com/2075-4418/10/11/923 |
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