Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of ase...
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| Format: | Article |
| Language: | English |
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BMC
2007-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007 |
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| author | S A SOUZA-JÚNIOR T C A BECKER M A A CASTRO-PRADO |
| author_facet | S A SOUZA-JÚNIOR T C A BECKER M A A CASTRO-PRADO |
| author_sort | S A SOUZA-JÚNIOR |
| collection | DOAJ |
| description | Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants originated through mitotic crossing-over and independent assortment of chromosomes |
| first_indexed | 2024-12-10T16:30:21Z |
| format | Article |
| id | doaj.art-6ba3f265592347fdbe6f4aaa86ebc3cf |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-10T16:30:21Z |
| publishDate | 2007-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-6ba3f265592347fdbe6f4aaa86ebc3cf2022-12-22T01:41:33ZengBMCBiological Research0716-97600717-62872007-01-014016571Asexual Recombination in a uvsH Mutant of Aspergillus nidulansS A SOUZA-JÚNIORT C A BECKERM A A CASTRO-PRADOMutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants originated through mitotic crossing-over and independent assortment of chromosomeshttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007mitotic crossing-overparameiosisuvs mutation |
| spellingShingle | S A SOUZA-JÚNIOR T C A BECKER M A A CASTRO-PRADO Asexual Recombination in a uvsH Mutant of Aspergillus nidulans Biological Research mitotic crossing-over parameiosis uvs mutation |
| title | Asexual Recombination in a uvsH Mutant of Aspergillus nidulans |
| title_full | Asexual Recombination in a uvsH Mutant of Aspergillus nidulans |
| title_fullStr | Asexual Recombination in a uvsH Mutant of Aspergillus nidulans |
| title_full_unstemmed | Asexual Recombination in a uvsH Mutant of Aspergillus nidulans |
| title_short | Asexual Recombination in a uvsH Mutant of Aspergillus nidulans |
| title_sort | asexual recombination in a uvsh mutant of aspergillus nidulans |
| topic | mitotic crossing-over parameiosis uvs mutation |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007 |
| work_keys_str_mv | AT sasouzajunior asexualrecombinationinauvshmutantofaspergillusnidulans AT tcabecker asexualrecombinationinauvshmutantofaspergillusnidulans AT maacastroprado asexualrecombinationinauvshmutantofaspergillusnidulans |