Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train
Abstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular transport in cilia, vital for cilia biogenesis and signaling. IFT-A plays crucial roles in the ciliary import of membrane proteins and the retr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-37208-2 |
| _version_ | 1827948057090588672 |
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| author | Yuanyuan Ma Jun He Shaobai Li Deqiang Yao Chenhui Huang Jian Wu Ming Lei |
| author_facet | Yuanyuan Ma Jun He Shaobai Li Deqiang Yao Chenhui Huang Jian Wu Ming Lei |
| author_sort | Yuanyuan Ma |
| collection | DOAJ |
| description | Abstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular transport in cilia, vital for cilia biogenesis and signaling. IFT-A plays crucial roles in the ciliary import of membrane proteins and the retrograde cargo trafficking. However, the molecular architecture of IFT-A and the assembly mechanism of the IFT-A into the IFT trains in vivo remains elusive. Here, we report the cryo-electron microscopic structures of the IFT-A complex from protozoa Tetrahymena thermophila. We find that IFT-A complexes present two distinct, elongated and folded states. Remarkably, comparison with the in situ cryo-electron tomography structure of the anterograde IFT train unveils a series of adjustments of the flexible arms in apo IFT-A when incorporated into the anterograde train. Our results provide an atomic-resolution model for the IFT-A complex and valuable insights into the assembly mechanism of anterograde IFT trains. |
| first_indexed | 2024-04-09T12:48:24Z |
| format | Article |
| id | doaj.art-6ba72c00a0bc4ed4a0a43d529f99c69e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T12:48:24Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6ba72c00a0bc4ed4a0a43d529f99c69e2023-05-14T11:20:54ZengNature PortfolioNature Communications2041-17232023-03-0114111210.1038/s41467-023-37208-2Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT trainYuanyuan Ma0Jun He1Shaobai Li2Deqiang Yao3Chenhui Huang4Jian Wu5Ming Lei6Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineNinth People’s Hospital, Shanghai Jiao Tong University School of MedicineNinth People’s Hospital, Shanghai Jiao Tong University School of MedicineRenji Hospital, Shanghai Jiao Tong University School of MedicineNinth People’s Hospital, Shanghai Jiao Tong University School of MedicineNinth People’s Hospital, Shanghai Jiao Tong University School of MedicineNinth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular transport in cilia, vital for cilia biogenesis and signaling. IFT-A plays crucial roles in the ciliary import of membrane proteins and the retrograde cargo trafficking. However, the molecular architecture of IFT-A and the assembly mechanism of the IFT-A into the IFT trains in vivo remains elusive. Here, we report the cryo-electron microscopic structures of the IFT-A complex from protozoa Tetrahymena thermophila. We find that IFT-A complexes present two distinct, elongated and folded states. Remarkably, comparison with the in situ cryo-electron tomography structure of the anterograde IFT train unveils a series of adjustments of the flexible arms in apo IFT-A when incorporated into the anterograde train. Our results provide an atomic-resolution model for the IFT-A complex and valuable insights into the assembly mechanism of anterograde IFT trains.https://doi.org/10.1038/s41467-023-37208-2 |
| spellingShingle | Yuanyuan Ma Jun He Shaobai Li Deqiang Yao Chenhui Huang Jian Wu Ming Lei Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train Nature Communications |
| title | Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train |
| title_full | Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train |
| title_fullStr | Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train |
| title_full_unstemmed | Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train |
| title_short | Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train |
| title_sort | structural insight into the intraflagellar transport complex ift a and its assembly in the anterograde ift train |
| url | https://doi.org/10.1038/s41467-023-37208-2 |
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