Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig
BackgroundTo minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified de...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Medical Publishers, Inc.
2013-01-01
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| Series: | Archives of Plastic Surgery |
| Subjects: | |
| Online Access: | http://www.e-aps.org/upload/pdf/aps-40-11.pdf |
| _version_ | 1798039252477411328 |
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| author | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han |
| author_facet | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han |
| author_sort | Hyunji Kim |
| collection | DOAJ |
| description | BackgroundTo minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type.MethodsThe AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively.ResultsMore rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group.ConclusionsThese observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing. |
| first_indexed | 2024-04-11T21:51:19Z |
| format | Article |
| id | doaj.art-6ba88f8e441844fb8ce8965603584bb7 |
| institution | Directory Open Access Journal |
| issn | 2234-6163 2234-6171 |
| language | English |
| last_indexed | 2024-04-11T21:51:19Z |
| publishDate | 2013-01-01 |
| publisher | Thieme Medical Publishers, Inc. |
| record_format | Article |
| series | Archives of Plastic Surgery |
| spelling | doaj.art-6ba88f8e441844fb8ce8965603584bb72022-12-22T04:01:14ZengThieme Medical Publishers, Inc.Archives of Plastic Surgery2234-61632234-61712013-01-014011118140Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a PigHyunji Kim0Daegu Son1Tae Hyun Choi2Samhyun Jung3Sunyoung Kwon4Junhyung Kim5Kihwan Han6Suibi Plastic Surgery Clinic, Daegu, Korea.Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea.Department of Tissue Engineering, Bioland, Cheongwon, Korea.Department of Pathology, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.BackgroundTo minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type.MethodsThe AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively.ResultsMore rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group.ConclusionsThese observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing.http://www.e-aps.org/upload/pdf/aps-40-11.pdfAmnionDermisBiologic dressingsCollagenChondroitin sulfates |
| spellingShingle | Hyunji Kim Daegu Son Tae Hyun Choi Samhyun Jung Sunyoung Kwon Junhyung Kim Kihwan Han Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig Archives of Plastic Surgery Amnion Dermis Biologic dressings Collagen Chondroitin sulfates |
| title | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_full | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_fullStr | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_full_unstemmed | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_short | Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig |
| title_sort | evaluation of an amniotic membrane collagen dermal substitute in the management of full thickness skin defects in a pig |
| topic | Amnion Dermis Biologic dressings Collagen Chondroitin sulfates |
| url | http://www.e-aps.org/upload/pdf/aps-40-11.pdf |
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