Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing
Omar B Ahmed Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute of Hajj and Umrah Research, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Omar B Ahmed, Email abuaglah1@hotmail.comBackground: Multidrug-resistant Pseudomonas aeruginosa has be...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-11-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/detection-of-antibiotic-resistance-genes-in-pseudomonas-aeruginosa-by--peer-reviewed-fulltext-article-IDR |
| _version_ | 1797986644584824832 |
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| author | Ahmed OB |
| author_facet | Ahmed OB |
| author_sort | Ahmed OB |
| collection | DOAJ |
| description | Omar B Ahmed Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute of Hajj and Umrah Research, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Omar B Ahmed, Email abuaglah1@hotmail.comBackground: Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers a powerful replacement for conventional microbiological methods.Objective: The present study evaluated the presence of antibiotic resistance genes in selected clinical strains of P. aeruginosa using whole-genome sequencing for antibiotic susceptibility testing.Results: Whole-genome sequencing of P. aeruginosa susceptible to common antibiotics showed the presence of 4 antibiotic resistance gene types, fosA, catB7, blaPAO, and blaOXA-50. Whole genome sequencing of resistant or multidrug-resistant P. aeruginosa showed the presence of multiple ARGs, such as sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph (3’)-XV, blaOXA-50, aacA4, catB7, aph(3’)-IIb, aadA6, fosA, tet(G), cmlA1, aac(6’)Ib-cr, and rmtF.Conclusion: The acquisition of antibiotic resistance genes was found to depend on the resistance of Pseudomonas to antibiotics. The strain with the highest resistance to antibiotics had the highest acquisition of antibiotic resistance genes. MDR-P. aeruginosa produces antibiotic resistance genes against aminoglycoside, β-lactam, fluoroquinolones, sulfonamides, phenicol, and fosfomycin antibiotics.Keywords: antibiotic resistance, genes, Pseudomonas aeruginosa, whole genome, sequencing |
| first_indexed | 2024-04-11T07:36:28Z |
| format | Article |
| id | doaj.art-6bab6af3a9374e5bb715e607d47a769c |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-11T07:36:28Z |
| publishDate | 2022-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-6bab6af3a9374e5bb715e607d47a769c2022-12-22T04:36:44ZengDove Medical PressInfection and Drug Resistance1178-69732022-11-01Volume 156703670979748Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome SequencingAhmed OBOmar B Ahmed Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute of Hajj and Umrah Research, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Omar B Ahmed, Email abuaglah1@hotmail.comBackground: Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers a powerful replacement for conventional microbiological methods.Objective: The present study evaluated the presence of antibiotic resistance genes in selected clinical strains of P. aeruginosa using whole-genome sequencing for antibiotic susceptibility testing.Results: Whole-genome sequencing of P. aeruginosa susceptible to common antibiotics showed the presence of 4 antibiotic resistance gene types, fosA, catB7, blaPAO, and blaOXA-50. Whole genome sequencing of resistant or multidrug-resistant P. aeruginosa showed the presence of multiple ARGs, such as sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph (3’)-XV, blaOXA-50, aacA4, catB7, aph(3’)-IIb, aadA6, fosA, tet(G), cmlA1, aac(6’)Ib-cr, and rmtF.Conclusion: The acquisition of antibiotic resistance genes was found to depend on the resistance of Pseudomonas to antibiotics. The strain with the highest resistance to antibiotics had the highest acquisition of antibiotic resistance genes. MDR-P. aeruginosa produces antibiotic resistance genes against aminoglycoside, β-lactam, fluoroquinolones, sulfonamides, phenicol, and fosfomycin antibiotics.Keywords: antibiotic resistance, genes, Pseudomonas aeruginosa, whole genome, sequencinghttps://www.dovepress.com/detection-of-antibiotic-resistance-genes-in-pseudomonas-aeruginosa-by--peer-reviewed-fulltext-article-IDRantibiotic resistancegenespseudomonas aeruginosawhole genomesequencing |
| spellingShingle | Ahmed OB Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing Infection and Drug Resistance antibiotic resistance genes pseudomonas aeruginosa whole genome sequencing |
| title | Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing |
| title_full | Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing |
| title_fullStr | Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing |
| title_full_unstemmed | Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing |
| title_short | Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing |
| title_sort | detection of antibiotic resistance genes in pseudomonas aeruginosa by whole genome sequencing |
| topic | antibiotic resistance genes pseudomonas aeruginosa whole genome sequencing |
| url | https://www.dovepress.com/detection-of-antibiotic-resistance-genes-in-pseudomonas-aeruginosa-by--peer-reviewed-fulltext-article-IDR |
| work_keys_str_mv | AT ahmedob detectionofantibioticresistancegenesinpseudomonasaeruginosabywholegenomesequencing |