Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system
Groundwater pollution is one of the major environmental concerns. The entrance of pollutants into the oligotrophic groundwater ecosystems alters native microbial community structure and metabolism. This study investigated the application of innovative Small Bioreactor Chambers and CaO2 nanoparticles...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1147162/full |
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| author | Maryam Yavari-Bafghi Maryam Rezaei Somee Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib Mahmoud Shavandi |
| author_facet | Maryam Yavari-Bafghi Maryam Rezaei Somee Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib Mahmoud Shavandi |
| author_sort | Maryam Yavari-Bafghi |
| collection | DOAJ |
| description | Groundwater pollution is one of the major environmental concerns. The entrance of pollutants into the oligotrophic groundwater ecosystems alters native microbial community structure and metabolism. This study investigated the application of innovative Small Bioreactor Chambers and CaO2 nanoparticles for phenol removal within continuous-flow sand-packed columns for 6 months. Scanning electron microscopy and confocal laser scanning microscopy analysis were conducted to indicate the impact of attached biofilm on sand surfaces in bioremediation columns. Then, the influence of each method on the microbial biodiversity of the column’s groundwater was investigated by next-generation sequencing of the 16S rRNA gene. The results indicated that the simultaneous application of biostimulation and bioaugmentation completely eliminated phenol during the first 42 days. However, 80.2% of phenol remained in the natural bioremediation column at the end of the experiment. Microbial diversity was decreased by CaO2 injection while order-level groups known for phenol degradation such as Rhodobacterales and Xanthomonadales dominated in biostimulation columns. Genome-resolved comparative analyses of oligotrophic groundwater prokaryotic communities revealed that Burkholderiales, Micrococcales, and Cytophagales were the dominant members of the pristine groundwater. Six-month exposure of groundwater to phenol shifted the microbial population towards increasing the heterotrophic members of Desulfobacterales, Pseudomonadales, and Xanthomonadales with the degradation potential of phenol and other hydrocarbons. |
| first_indexed | 2024-04-09T21:04:04Z |
| format | Article |
| id | doaj.art-6bac89eedbc04650b611e2e2e91645b1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T21:04:04Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6bac89eedbc04650b611e2e2e91645b12023-03-29T04:56:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11471621147162Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model systemMaryam Yavari-Bafghi0Maryam Rezaei Somee1Mohammad Ali Amoozegar2Seyed Mohammad Mehdi Dastgheib3Mahmoud Shavandi4Extremophiles Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IranCentre for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, Kalmar, SwedenExtremophiles Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IranMicrobiology and Biotechnology Group, Environment and Biotechnology Research Division, Research Institute of Petroleum Industry, Tehran, IranMicrobiology and Biotechnology Group, Environment and Biotechnology Research Division, Research Institute of Petroleum Industry, Tehran, IranGroundwater pollution is one of the major environmental concerns. The entrance of pollutants into the oligotrophic groundwater ecosystems alters native microbial community structure and metabolism. This study investigated the application of innovative Small Bioreactor Chambers and CaO2 nanoparticles for phenol removal within continuous-flow sand-packed columns for 6 months. Scanning electron microscopy and confocal laser scanning microscopy analysis were conducted to indicate the impact of attached biofilm on sand surfaces in bioremediation columns. Then, the influence of each method on the microbial biodiversity of the column’s groundwater was investigated by next-generation sequencing of the 16S rRNA gene. The results indicated that the simultaneous application of biostimulation and bioaugmentation completely eliminated phenol during the first 42 days. However, 80.2% of phenol remained in the natural bioremediation column at the end of the experiment. Microbial diversity was decreased by CaO2 injection while order-level groups known for phenol degradation such as Rhodobacterales and Xanthomonadales dominated in biostimulation columns. Genome-resolved comparative analyses of oligotrophic groundwater prokaryotic communities revealed that Burkholderiales, Micrococcales, and Cytophagales were the dominant members of the pristine groundwater. Six-month exposure of groundwater to phenol shifted the microbial population towards increasing the heterotrophic members of Desulfobacterales, Pseudomonadales, and Xanthomonadales with the degradation potential of phenol and other hydrocarbons.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1147162/fulloligotrophic groundwaterbiodiversitymetagenomephenolbioremediation |
| spellingShingle | Maryam Yavari-Bafghi Maryam Rezaei Somee Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib Mahmoud Shavandi Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system Frontiers in Microbiology oligotrophic groundwater biodiversity metagenome phenol bioremediation |
| title | Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system |
| title_full | Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system |
| title_fullStr | Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system |
| title_full_unstemmed | Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system |
| title_short | Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system |
| title_sort | genome resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous flow biodegradation model system |
| topic | oligotrophic groundwater biodiversity metagenome phenol bioremediation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1147162/full |
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