Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies
Although, historically, Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) was restricted to humans, since 2005 these strains emerged in livestock and wildlife. Therefore, a One Health approach was applied to analyze the diversity and characteristics of <i>S. aureus</i>...
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MDPI AG
2022-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/8/990 |
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| author | Andreas Hoefer Anne A. M. J. Becker Arshnee Moodley Filip Boyen Patrick Butaye |
| author_facet | Andreas Hoefer Anne A. M. J. Becker Arshnee Moodley Filip Boyen Patrick Butaye |
| author_sort | Andreas Hoefer |
| collection | DOAJ |
| description | Although, historically, Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) was restricted to humans, since 2005 these strains emerged in livestock and wildlife. Therefore, a One Health approach was applied to analyze the diversity and characteristics of <i>S. aureus</i> strains isolated from the invasive species of mongoose (<i>Urva auropunctata</i>) in St. Kitts. Fecal samples collected from these animals (<i>n</i> = 81) were cultured on selective agar. The isolated <i>S. aureus</i> strains were identified using MALDI-TOF and further characterized by whole genome sequence analysis. The fecal microbiome study identified the presence of <i>S. aureus</i> in 5 animals. Both MSSA (<i>n</i> = 3) and MRSA (<i>n</i> = 2) strains were identified. The two MRSA isolated were nearly identical ST5 SCC<i>mec</i> IVa (2B) strains. The two MSSA isolated were a new ST7434, pertaining to clonal complex 30, and the other belonged to ST5, but unrelated to the MRSA ST5. The SCC<i>mec</i> IVa (2B) is, however, the main SCC<i>mec</i> in human MRSA of different STs identified in St Kitts, indicating potential horizontal transmission events. In conclusion, a new type of MSSA, ST7434, was found and MRSA ST5 t002 SCC<i>mec</i> IVa (2B) found its way into wildlife on a small Caribbean Island. Further One Health studies are necessary to determine the role of MRSA in wildlife. |
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| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T04:47:54Z |
| publishDate | 2022-07-01 |
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| series | Antibiotics |
| spelling | doaj.art-6bad34f0ffb84a3ebc961aa43aff0b762023-12-03T13:14:04ZengMDPI AGAntibiotics2079-63822022-07-0111899010.3390/antibiotics11080990Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West IndiesAndreas Hoefer0Anne A. M. J. Becker1Arshnee Moodley2Filip Boyen3Patrick Butaye4School of Veterinary Medicine, Ross University, Basseterre P.O. Box 334, Saint Kitts and NevisSchool of Veterinary Medicine, Ross University, Basseterre P.O. Box 334, Saint Kitts and NevisInternational Livestock Research Institute, Animal and Human Health, Nairobi 00100, KenyaDepartment of Pathobiology, Pharmacology and Wildlife Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumSchool of Veterinary Medicine, Ross University, Basseterre P.O. Box 334, Saint Kitts and NevisAlthough, historically, Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) was restricted to humans, since 2005 these strains emerged in livestock and wildlife. Therefore, a One Health approach was applied to analyze the diversity and characteristics of <i>S. aureus</i> strains isolated from the invasive species of mongoose (<i>Urva auropunctata</i>) in St. Kitts. Fecal samples collected from these animals (<i>n</i> = 81) were cultured on selective agar. The isolated <i>S. aureus</i> strains were identified using MALDI-TOF and further characterized by whole genome sequence analysis. The fecal microbiome study identified the presence of <i>S. aureus</i> in 5 animals. Both MSSA (<i>n</i> = 3) and MRSA (<i>n</i> = 2) strains were identified. The two MRSA isolated were nearly identical ST5 SCC<i>mec</i> IVa (2B) strains. The two MSSA isolated were a new ST7434, pertaining to clonal complex 30, and the other belonged to ST5, but unrelated to the MRSA ST5. The SCC<i>mec</i> IVa (2B) is, however, the main SCC<i>mec</i> in human MRSA of different STs identified in St Kitts, indicating potential horizontal transmission events. In conclusion, a new type of MSSA, ST7434, was found and MRSA ST5 t002 SCC<i>mec</i> IVa (2B) found its way into wildlife on a small Caribbean Island. Further One Health studies are necessary to determine the role of MRSA in wildlife.https://www.mdpi.com/2079-6382/11/8/990MRSAwildlife<i>Staphylococcus aureus</i>whole genome sequencingOne Health |
| spellingShingle | Andreas Hoefer Anne A. M. J. Becker Arshnee Moodley Filip Boyen Patrick Butaye Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies Antibiotics MRSA wildlife <i>Staphylococcus aureus</i> whole genome sequencing One Health |
| title | Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies |
| title_full | Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies |
| title_fullStr | Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies |
| title_full_unstemmed | Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies |
| title_short | Presence of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) in Feces of the Small Indian Mongooses (<i>Urva auropunctata</i>) on Saint Kitts and Nevis, West Indies |
| title_sort | presence of methicillin resistant i staphylococcus aureus i mrsa in feces of the small indian mongooses i urva auropunctata i on saint kitts and nevis west indies |
| topic | MRSA wildlife <i>Staphylococcus aureus</i> whole genome sequencing One Health |
| url | https://www.mdpi.com/2079-6382/11/8/990 |
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