Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites
Two-dimensional nanosilicate particles (NS) have shown promise for the prolonged release of small-molecule therapeutics while minimizing burst release. When incorporated in a hydrogel, the high surface area and charge of NS enable electrostatic adsorption and/or intercalation of therapeutics, provid...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
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| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/15/1/56 |
| _version_ | 1797491275099799552 |
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| author | Samuel Stealey Mariam Khachani Silviya Petrova Zustiak |
| author_facet | Samuel Stealey Mariam Khachani Silviya Petrova Zustiak |
| author_sort | Samuel Stealey |
| collection | DOAJ |
| description | Two-dimensional nanosilicate particles (NS) have shown promise for the prolonged release of small-molecule therapeutics while minimizing burst release. When incorporated in a hydrogel, the high surface area and charge of NS enable electrostatic adsorption and/or intercalation of therapeutics, providing a lever to localize and control release. However, little is known about the physio-chemical interplay between the hydrogel, NS, and encapsulated small molecules. Here, we fabricated polyethylene glycol (PEG)-NS hydrogels for the release of model small molecules such as acridine orange (AO). We then elucidated the effect of NS concentration, NS/AO incubation time, and the ability of NS to freely associate with AO on hydrogel properties and AO release profiles. Overall, NS incorporation increased the hydrogel stiffness and decreased swelling and mesh size. When individual NS particles were embedded within the hydrogel, a 70-fold decrease in AO release was observed compared to PEG-only hydrogels, due to adsorption of AO onto NS surfaces. When NS was pre-incubated and complexed with AO prior to hydrogel encapsulation, a >9000-fold decrease in AO release was observed due to intercalation of AO between NS layers. Similar results were observed for other small molecules. Our results show the potential for use of these nanocomposite hydrogels for the tunable, long-term release of small molecules. |
| first_indexed | 2024-03-10T00:45:07Z |
| format | Article |
| id | doaj.art-6bad7ad1fa614e5ba61206c707df6807 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T00:45:07Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-6bad7ad1fa614e5ba61206c707df68072023-11-23T15:01:24ZengMDPI AGPharmaceuticals1424-82472022-01-011515610.3390/ph15010056Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel CompositesSamuel Stealey0Mariam Khachani1Silviya Petrova Zustiak2Biomedical Engineering Program, Parks College of Engineering, Saint Louis University, Saint Louis, MO 63103, USABiomedical Engineering Program, Parks College of Engineering, Saint Louis University, Saint Louis, MO 63103, USABiomedical Engineering Program, Parks College of Engineering, Saint Louis University, Saint Louis, MO 63103, USATwo-dimensional nanosilicate particles (NS) have shown promise for the prolonged release of small-molecule therapeutics while minimizing burst release. When incorporated in a hydrogel, the high surface area and charge of NS enable electrostatic adsorption and/or intercalation of therapeutics, providing a lever to localize and control release. However, little is known about the physio-chemical interplay between the hydrogel, NS, and encapsulated small molecules. Here, we fabricated polyethylene glycol (PEG)-NS hydrogels for the release of model small molecules such as acridine orange (AO). We then elucidated the effect of NS concentration, NS/AO incubation time, and the ability of NS to freely associate with AO on hydrogel properties and AO release profiles. Overall, NS incorporation increased the hydrogel stiffness and decreased swelling and mesh size. When individual NS particles were embedded within the hydrogel, a 70-fold decrease in AO release was observed compared to PEG-only hydrogels, due to adsorption of AO onto NS surfaces. When NS was pre-incubated and complexed with AO prior to hydrogel encapsulation, a >9000-fold decrease in AO release was observed due to intercalation of AO between NS layers. Similar results were observed for other small molecules. Our results show the potential for use of these nanocomposite hydrogels for the tunable, long-term release of small molecules.https://www.mdpi.com/1424-8247/15/1/56laponitepolyethylene glycol hydrogelacridine orangetherapeutic deliverynanocompositeintercalation |
| spellingShingle | Samuel Stealey Mariam Khachani Silviya Petrova Zustiak Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites Pharmaceuticals laponite polyethylene glycol hydrogel acridine orange therapeutic delivery nanocomposite intercalation |
| title | Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites |
| title_full | Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites |
| title_fullStr | Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites |
| title_full_unstemmed | Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites |
| title_short | Adsorption and Sustained Delivery of Small Molecules from Nanosilicate Hydrogel Composites |
| title_sort | adsorption and sustained delivery of small molecules from nanosilicate hydrogel composites |
| topic | laponite polyethylene glycol hydrogel acridine orange therapeutic delivery nanocomposite intercalation |
| url | https://www.mdpi.com/1424-8247/15/1/56 |
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