Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats
Abstract Objectives: To identify the phytoconstituents and determine the effect of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats. Methods: Phytochemical screening was performed using the drop Test. For hepatoxicity study, albino rats were randomized into...
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Facultad de Farmacia, Universidad de Granada
2020-08-01
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| Series: | Ars Pharmaceutica |
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| Online Access: | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942020000300006&tlng=en |
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| author | Leyly Chávez-Alcántara Yesica Cabanillas-Vargas Segundo Ruiz-Reyes Ana María Guevara-Vásquez |
| author_facet | Leyly Chávez-Alcántara Yesica Cabanillas-Vargas Segundo Ruiz-Reyes Ana María Guevara-Vásquez |
| author_sort | Leyly Chávez-Alcántara |
| collection | DOAJ |
| description | Abstract Objectives: To identify the phytoconstituents and determine the effect of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats. Methods: Phytochemical screening was performed using the drop Test. For hepatoxicity study, albino rats were randomized into 5 groups of 6 each. Healthy group: without hepatoxicity; Control group: hepatotoxicity; Curative group: hepatotoxicity plus fluid extract of N. albopunctatissimum Lellinger; Standard group: hepatotoxicity plus silymarin; Preventive group: Fluid extract of N. albopunctatissimum Lellinger for one week, then hepatotoxicity. Hepatotoxicity was induced with 56% (w / v) ethanol at doses of 7.6 mL / kg p.c every 12 hours for 7 days. Glutamic pyruvic transaminase (GPT) values were determined at baseline, 7 days after induction and 14 days after. A histopathological study of the livers was performed. Results: Phytochemical screening revealed the presence of polyphenols, anthocyanidins, saponins, flavonoids and tannins. The preventive and standard groups showed a very significant decrease in serum GPT levels compared to control group (p <0.01), and curative group did so significantly (p <0.05). Histopathological analysis showed in curative group some degenerating hepatocytes, many with normal-looking cytoplasm; the preventive and standard groups presented hepatocytes with normal architecture and some in degeneration, unlike the evident degeneration and necrosis in control group. Conclusión: Niphidium albopunctatissimum Lellinger may have hepatoprotective potential against alcoholic toxicity induced in albino rats. |
| first_indexed | 2024-04-11T20:28:09Z |
| format | Article |
| id | doaj.art-6bae1ae7ce4c473f9fc55883f03f1648 |
| institution | Directory Open Access Journal |
| issn | 2340-9894 |
| language | English |
| last_indexed | 2024-04-11T20:28:09Z |
| publishDate | 2020-08-01 |
| publisher | Facultad de Farmacia, Universidad de Granada |
| record_format | Article |
| series | Ars Pharmaceutica |
| spelling | doaj.art-6bae1ae7ce4c473f9fc55883f03f16482022-12-22T04:04:35ZengFacultad de Farmacia, Universidad de GranadaArs Pharmaceutica2340-98942020-08-0161318719210.30827/ars.v61i3.13634Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino ratsLeyly Chávez-AlcántaraYesica Cabanillas-VargasSegundo Ruiz-ReyesAna María Guevara-VásquezAbstract Objectives: To identify the phytoconstituents and determine the effect of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats. Methods: Phytochemical screening was performed using the drop Test. For hepatoxicity study, albino rats were randomized into 5 groups of 6 each. Healthy group: without hepatoxicity; Control group: hepatotoxicity; Curative group: hepatotoxicity plus fluid extract of N. albopunctatissimum Lellinger; Standard group: hepatotoxicity plus silymarin; Preventive group: Fluid extract of N. albopunctatissimum Lellinger for one week, then hepatotoxicity. Hepatotoxicity was induced with 56% (w / v) ethanol at doses of 7.6 mL / kg p.c every 12 hours for 7 days. Glutamic pyruvic transaminase (GPT) values were determined at baseline, 7 days after induction and 14 days after. A histopathological study of the livers was performed. Results: Phytochemical screening revealed the presence of polyphenols, anthocyanidins, saponins, flavonoids and tannins. The preventive and standard groups showed a very significant decrease in serum GPT levels compared to control group (p <0.01), and curative group did so significantly (p <0.05). Histopathological analysis showed in curative group some degenerating hepatocytes, many with normal-looking cytoplasm; the preventive and standard groups presented hepatocytes with normal architecture and some in degeneration, unlike the evident degeneration and necrosis in control group. Conclusión: Niphidium albopunctatissimum Lellinger may have hepatoprotective potential against alcoholic toxicity induced in albino rats.http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942020000300006&tlng=enPhytochemicalsLiverPlant extractsEthanol |
| spellingShingle | Leyly Chávez-Alcántara Yesica Cabanillas-Vargas Segundo Ruiz-Reyes Ana María Guevara-Vásquez Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats Ars Pharmaceutica Phytochemicals Liver Plant extracts Ethanol |
| title | Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats |
| title_full | Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats |
| title_fullStr | Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats |
| title_full_unstemmed | Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats |
| title_short | Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats |
| title_sort | phytochemical screening and hepatoprotective potential of niphidium albopunctatissimum lellinger on alcoholic hepatoxicity induced in albino rats |
| topic | Phytochemicals Liver Plant extracts Ethanol |
| url | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S2340-98942020000300006&tlng=en |
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