Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers
The aim of the study was to identify the aerobic bacterial isolates and determine corresponding antibiotic susceptibility profiles in vitro in canine clinical specimens with stromal corneal ulcers, with the goal of providing recommendations for first-line treatment with antibiotics. A total of 198 c...
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MDPI AG
2023-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/2/66 |
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| author | Milan Joksimovic Bradley A. Ford Tatjana Lazic Ivan Soldatovic Sergey Luzetsky Sinisa Grozdanic |
| author_facet | Milan Joksimovic Bradley A. Ford Tatjana Lazic Ivan Soldatovic Sergey Luzetsky Sinisa Grozdanic |
| author_sort | Milan Joksimovic |
| collection | DOAJ |
| description | The aim of the study was to identify the aerobic bacterial isolates and determine corresponding antibiotic susceptibility profiles in vitro in canine clinical specimens with stromal corneal ulcers, with the goal of providing recommendations for first-line treatment with antibiotics. A total of 198 canine corneal stromal ulcer samples were studied between 2018 and 2021. A corneal swab was collected and cultured under aerobic conditions. Bacterial organisms were identified at the species level by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing for commonly used topical and systemic antibiotics was performed by disk diffusion. Bacterial growth was obtained from 80% of samples. A variety of bacterial species were identified wherein the most common specimens were represented by <i>Staphylococcus pseudintermedius</i> (22%), <i>Staphylococcus epidermidis</i> (12%), <i>Staphylococcus capitis</i> (11%), and <i>Pseudomonas aeruginosa</i> (10%). Based on the overall antibiotic susceptibility data, neopolybac alone (96%) or a combination of neopolybac with either ofloxacin or amikacin (each 99%) showed the best coverage for commonly isolated bacterial organisms from canine corneal stromal ulcers. Results of this study support the use of the combined antibiotics as the first-line response for the treatment of canine corneal stromal ulcers. A statically significant increase in acquired bacterial resistance was detected during the longitudinal data observation. |
| first_indexed | 2024-03-11T08:01:41Z |
| format | Article |
| id | doaj.art-6bb2c81bc2cc4e30a59520ee27fe4df3 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T08:01:41Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-6bb2c81bc2cc4e30a59520ee27fe4df32023-11-16T23:45:10ZengMDPI AGVeterinary Sciences2306-73812023-01-011026610.3390/vetsci10020066Antibiotic Recommendations for Treatment of Canine Stromal Corneal UlcersMilan Joksimovic0Bradley A. Ford1Tatjana Lazic2Ivan Soldatovic3Sergey Luzetsky4Sinisa Grozdanic5Animal Eye Consultants of Iowa, 698 Boyson Road, Hiawatha, IA 52233, USADepartment of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAAnimal Eye Consultants of Iowa, 698 Boyson Road, Hiawatha, IA 52233, USADepartment of Biostatistics, College of Medicine, University of Belgrade, 11000 Belgrade, SerbiaOculus–Ophthalmology Specialty Hospital, 11000 Belgrade, SerbiaAnimal Eye Consultants of Iowa, 698 Boyson Road, Hiawatha, IA 52233, USAThe aim of the study was to identify the aerobic bacterial isolates and determine corresponding antibiotic susceptibility profiles in vitro in canine clinical specimens with stromal corneal ulcers, with the goal of providing recommendations for first-line treatment with antibiotics. A total of 198 canine corneal stromal ulcer samples were studied between 2018 and 2021. A corneal swab was collected and cultured under aerobic conditions. Bacterial organisms were identified at the species level by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing for commonly used topical and systemic antibiotics was performed by disk diffusion. Bacterial growth was obtained from 80% of samples. A variety of bacterial species were identified wherein the most common specimens were represented by <i>Staphylococcus pseudintermedius</i> (22%), <i>Staphylococcus epidermidis</i> (12%), <i>Staphylococcus capitis</i> (11%), and <i>Pseudomonas aeruginosa</i> (10%). Based on the overall antibiotic susceptibility data, neopolybac alone (96%) or a combination of neopolybac with either ofloxacin or amikacin (each 99%) showed the best coverage for commonly isolated bacterial organisms from canine corneal stromal ulcers. Results of this study support the use of the combined antibiotics as the first-line response for the treatment of canine corneal stromal ulcers. A statically significant increase in acquired bacterial resistance was detected during the longitudinal data observation.https://www.mdpi.com/2306-7381/10/2/66cornealstromalulcerbacterialcaninesantibiotic |
| spellingShingle | Milan Joksimovic Bradley A. Ford Tatjana Lazic Ivan Soldatovic Sergey Luzetsky Sinisa Grozdanic Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers Veterinary Sciences corneal stromal ulcer bacterial canines antibiotic |
| title | Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers |
| title_full | Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers |
| title_fullStr | Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers |
| title_full_unstemmed | Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers |
| title_short | Antibiotic Recommendations for Treatment of Canine Stromal Corneal Ulcers |
| title_sort | antibiotic recommendations for treatment of canine stromal corneal ulcers |
| topic | corneal stromal ulcer bacterial canines antibiotic |
| url | https://www.mdpi.com/2306-7381/10/2/66 |
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