Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome
Multi-InDel, as the novel genetic markers, showed great potential in forensic research. Whereas, most scholars mainly focused on autosomal Multi-InDels, which might provide limited genetic information in some complex kinship cases. In this study, we selected 17 Multi-InDels on the X chromosome and d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2022.985933/full |
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| author | Xiaoye Jin Xiaoye Jin Zheng Ren Hongling Zhang Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Wen Hu Ning Wang Yicong Wang Jiang Huang |
| author_facet | Xiaoye Jin Xiaoye Jin Zheng Ren Hongling Zhang Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Wen Hu Ning Wang Yicong Wang Jiang Huang |
| author_sort | Xiaoye Jin |
| collection | DOAJ |
| description | Multi-InDel, as the novel genetic markers, showed great potential in forensic research. Whereas, most scholars mainly focused on autosomal Multi-InDels, which might provide limited genetic information in some complex kinship cases. In this study, we selected 17 Multi-InDels on the X chromosome and developed a multiplex amplification panel based on the next-generation sequencing (NGS) technology. Genetic distributions of these 17 loci in Beijing Han, Chinese Southern Han, and the studied Guizhou Han populations revealed that most loci showed relatively high forensic application values in these Han populations. In addition, more allelic variations of some loci were observed in the Guizhou Han than those in Beijing Han and Southern Han populations. Pairwise FST values, multi-dimensional analysis, and phylogenetic tree of different continental populations showed that selected 17 loci generally could differentiate African, European, East Asian, and South Asian populations. To sum up, the developed panel in this study is not only viewed as the high-efficient supplementary tool for forensic individual identification and paternity analysis, but it is also beneficial for inferring biogeographical origins of different continental populations. |
| first_indexed | 2024-04-12T16:03:21Z |
| format | Article |
| id | doaj.art-6bb30f7b463c4714981e31540a20324b |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-04-12T16:03:21Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-6bb30f7b463c4714981e31540a20324b2022-12-22T03:26:09ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2022-10-011010.3389/fevo.2022.985933985933Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosomeXiaoye Jin0Xiaoye Jin1Zheng Ren2Hongling Zhang3Qiyan Wang4Yubo Liu5Jingyan Ji6Meiqing Yang7Han Zhang8Wen Hu9Ning Wang10Yicong Wang11Jiang Huang12Department of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaShanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, Shanghai, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaBGI-Shenzhen, Shenzhen, ChinaBGI-Shenzhen, Shenzhen, ChinaBGI-Shenzhen, Shenzhen, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaMulti-InDel, as the novel genetic markers, showed great potential in forensic research. Whereas, most scholars mainly focused on autosomal Multi-InDels, which might provide limited genetic information in some complex kinship cases. In this study, we selected 17 Multi-InDels on the X chromosome and developed a multiplex amplification panel based on the next-generation sequencing (NGS) technology. Genetic distributions of these 17 loci in Beijing Han, Chinese Southern Han, and the studied Guizhou Han populations revealed that most loci showed relatively high forensic application values in these Han populations. In addition, more allelic variations of some loci were observed in the Guizhou Han than those in Beijing Han and Southern Han populations. Pairwise FST values, multi-dimensional analysis, and phylogenetic tree of different continental populations showed that selected 17 loci generally could differentiate African, European, East Asian, and South Asian populations. To sum up, the developed panel in this study is not only viewed as the high-efficient supplementary tool for forensic individual identification and paternity analysis, but it is also beneficial for inferring biogeographical origins of different continental populations.https://www.frontiersin.org/articles/10.3389/fevo.2022.985933/fullMulti-InDelX chromosomeNGSforensic researchbiogeographical origin inference |
| spellingShingle | Xiaoye Jin Xiaoye Jin Zheng Ren Hongling Zhang Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Wen Hu Ning Wang Yicong Wang Jiang Huang Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome Frontiers in Ecology and Evolution Multi-InDel X chromosome NGS forensic research biogeographical origin inference |
| title | Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome |
| title_full | Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome |
| title_fullStr | Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome |
| title_full_unstemmed | Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome |
| title_short | Development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 Multi-InDel loci on the X chromosome |
| title_sort | development and forensic efficiency evaluations of a novel multiplex amplification panel of 17 multi indel loci on the x chromosome |
| topic | Multi-InDel X chromosome NGS forensic research biogeographical origin inference |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2022.985933/full |
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