Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia
Abstract Objective Hydrogen sulfide (H2S) has been found to act as an important gasotransmitter to regulate cell activities. This study aimed to investigate the effect of H2S on autophagy of nucleus pulposus (NP) cells under hypoxia and possible mechanism. Materials and Methods NP cells were isolate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-12-01
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| Series: | JOR Spine |
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| Online Access: | https://doi.org/10.1002/jsp2.1181 |
| _version_ | 1798033369925156864 |
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| author | Lei Yue Yongkai Hu Haoyong Fu Longtao Qi Haolin Sun |
| author_facet | Lei Yue Yongkai Hu Haoyong Fu Longtao Qi Haolin Sun |
| author_sort | Lei Yue |
| collection | DOAJ |
| description | Abstract Objective Hydrogen sulfide (H2S) has been found to act as an important gasotransmitter to regulate cell activities. This study aimed to investigate the effect of H2S on autophagy of nucleus pulposus (NP) cells under hypoxia and possible mechanism. Materials and Methods NP cells were isolated from rat caudal discs. Cobalt chloride was used to mimic hypoxia, sodium hydrosulfide was used to emulate exogenous H2S and 3‐methyladenine was used to block cell autophagy. Cell viability was assessed by phase contrast microscope and Cell Counting Kit‐8 method. Moreover, expression of key autophagic proteins was analyzed via western blotting, and transmission electron microscopy was performed to detect autophagosomes. Results Hypoxia markedly impaired NP cell proliferation compared with control. Whereas H2S provided pro‐proliferation and pro‐autophagy effects on hypoxic NP cells. However, these beneficial impact of H2S on hypoxic NP cells were reversed by autophagy inhibitor. Conclusions Our results showed that H2S played a cytoprotective role in NP cells exposed to hypoxia in an autophagy‐dependent manner. |
| first_indexed | 2024-04-11T20:29:15Z |
| format | Article |
| id | doaj.art-6bb6d5f8e88449848b6d17ce344da72d |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-04-11T20:29:15Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-6bb6d5f8e88449848b6d17ce344da72d2022-12-22T04:04:34ZengWileyJOR Spine2572-11432021-12-0144n/an/a10.1002/jsp2.1181Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxiaLei Yue0Yongkai Hu1Haoyong Fu2Longtao Qi3Haolin Sun4Department of Orthopaedics Peking University First Hospital, Peking University Beijing ChinaDepartment of Orthopedics General Hospital of Southern Theatre Command of PLA Guangzhou ChinaDepartment of Orthopaedics Peking University First Hospital, Peking University Beijing ChinaDepartment of Orthopaedics Peking University First Hospital, Peking University Beijing ChinaDepartment of Orthopaedics Peking University First Hospital, Peking University Beijing ChinaAbstract Objective Hydrogen sulfide (H2S) has been found to act as an important gasotransmitter to regulate cell activities. This study aimed to investigate the effect of H2S on autophagy of nucleus pulposus (NP) cells under hypoxia and possible mechanism. Materials and Methods NP cells were isolated from rat caudal discs. Cobalt chloride was used to mimic hypoxia, sodium hydrosulfide was used to emulate exogenous H2S and 3‐methyladenine was used to block cell autophagy. Cell viability was assessed by phase contrast microscope and Cell Counting Kit‐8 method. Moreover, expression of key autophagic proteins was analyzed via western blotting, and transmission electron microscopy was performed to detect autophagosomes. Results Hypoxia markedly impaired NP cell proliferation compared with control. Whereas H2S provided pro‐proliferation and pro‐autophagy effects on hypoxic NP cells. However, these beneficial impact of H2S on hypoxic NP cells were reversed by autophagy inhibitor. Conclusions Our results showed that H2S played a cytoprotective role in NP cells exposed to hypoxia in an autophagy‐dependent manner.https://doi.org/10.1002/jsp2.1181autophagyhydrogen sulfidenucleus pulposus |
| spellingShingle | Lei Yue Yongkai Hu Haoyong Fu Longtao Qi Haolin Sun Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia JOR Spine autophagy hydrogen sulfide nucleus pulposus |
| title | Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| title_full | Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| title_fullStr | Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| title_full_unstemmed | Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| title_short | Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| title_sort | hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia |
| topic | autophagy hydrogen sulfide nucleus pulposus |
| url | https://doi.org/10.1002/jsp2.1181 |
| work_keys_str_mv | AT leiyue hydrogensulfideregulatesautophagyinnucleuspulposuscellsunderhypoxia AT yongkaihu hydrogensulfideregulatesautophagyinnucleuspulposuscellsunderhypoxia AT haoyongfu hydrogensulfideregulatesautophagyinnucleuspulposuscellsunderhypoxia AT longtaoqi hydrogensulfideregulatesautophagyinnucleuspulposuscellsunderhypoxia AT haolinsun hydrogensulfideregulatesautophagyinnucleuspulposuscellsunderhypoxia |