Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats
Objectives: The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiment...
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| Format: | Article |
| Language: | English |
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São Paulo State University (UNESP)
2021-03-01
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| Series: | Revista de Ciências Farmacêuticas Básica e Aplicada |
| Subjects: | |
| Online Access: | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/711 |
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| author | Alessandra dos Santos Danziger Silvério Rosemary Gualberto Fonseca Alvarenga Pereira Stella Maris da Silveira Duarte Sônia Aparecida Figueiredo Fernanda Borges de Araújo Paula Tomaz Henrique Araújo Cláudia de Souza Ferreira Bruno César Corrêa Salles André Luiz Machado Viana Eric Batista Ferreira Maria Rita Rodrigues |
| author_facet | Alessandra dos Santos Danziger Silvério Rosemary Gualberto Fonseca Alvarenga Pereira Stella Maris da Silveira Duarte Sônia Aparecida Figueiredo Fernanda Borges de Araújo Paula Tomaz Henrique Araújo Cláudia de Souza Ferreira Bruno César Corrêa Salles André Luiz Machado Viana Eric Batista Ferreira Maria Rita Rodrigues |
| author_sort | Alessandra dos Santos Danziger Silvério |
| collection | DOAJ |
| description | Objectives: The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiments were approved by the ethics committee for animal experiments under N°. 420/2012 and 536/2013. Diabetic and non-diabetic rats were divided into 6 groups treated and untreated with coffee (7.2 mL/Kg body weight) and aminoguanidine (AGE inhibiting agent) (100 mg/Kg body weight) for 50 days. After 50 days, the animals were fasted for 12 h and anesthetized (40 mg/Kg sodium pentobarbital) intraperitoneally. Blood samples were collected from the abdominal artery puncture. Hematological parameters (red cells, hemoglobin, hematocrit and leukocyte) and glycemic and HbA1c levels were measured. AGEs quantification (spectrofluorometric method) and the platelet aggregation test (aggregation of cuvettes in a four-channel platelet aggregometer) were also conducted. The rats’ renal function was evaluated by measuring serum urea and creatinine. Results: Data showed that coffee intake had no effect on the hematological parameters. Fasting glucose and HbA1c dosage were significantly higher in diabetic animals compared to non-diabetic animals (confirmed the effectiveness of inducing and maintaining diabetic status). Results showed that coffee reduced AGE formation and platelet aggregation in our animal model, not altering the animals’ renal function. Conclusions: These results suggest beneficial effects on vasculopathy, a common complication in diabetic patients. |
| first_indexed | 2024-12-20T10:58:16Z |
| format | Article |
| id | doaj.art-6bb871f09d0d4d04bf59a85d78be746c |
| institution | Directory Open Access Journal |
| issn | 1808-4532 2179-443X |
| language | English |
| last_indexed | 2024-12-20T10:58:16Z |
| publishDate | 2021-03-01 |
| publisher | São Paulo State University (UNESP) |
| record_format | Article |
| series | Revista de Ciências Farmacêuticas Básica e Aplicada |
| spelling | doaj.art-6bb871f09d0d4d04bf59a85d78be746c2022-12-21T19:43:05ZengSão Paulo State University (UNESP)Revista de Ciências Farmacêuticas Básica e Aplicada1808-45322179-443X2021-03-0142114711Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic ratsAlessandra dos Santos Danziger Silvério0Rosemary Gualberto Fonseca Alvarenga Pereira1Stella Maris da Silveira Duarte2Sônia Aparecida Figueiredo3Fernanda Borges de Araújo Paula4Tomaz Henrique Araújo5Cláudia de Souza Ferreira6Bruno César Corrêa Salles7André Luiz Machado Viana8Eric Batista Ferreira9Maria Rita Rodrigues10Faculdade de Medicina, Universidade José do Rosário Vellano, Unifenas, Alfenas, MG, Brasil Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Lavras, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilInstituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilInstituto de Ciências Exatas, Universidade Federal de Alfenas, Alfenas, MG, BrasilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG, BrasilObjectives: The study aimed to determine the effect of coffee intake on AGEs formation and platelet aggregation in diabetic Wistar rats. Methods: Coffee powder samples were used to prepare a 10% beverage. Diabetes mellitus was induced in the animals by administering 2% alloxan. All animal experiments were approved by the ethics committee for animal experiments under N°. 420/2012 and 536/2013. Diabetic and non-diabetic rats were divided into 6 groups treated and untreated with coffee (7.2 mL/Kg body weight) and aminoguanidine (AGE inhibiting agent) (100 mg/Kg body weight) for 50 days. After 50 days, the animals were fasted for 12 h and anesthetized (40 mg/Kg sodium pentobarbital) intraperitoneally. Blood samples were collected from the abdominal artery puncture. Hematological parameters (red cells, hemoglobin, hematocrit and leukocyte) and glycemic and HbA1c levels were measured. AGEs quantification (spectrofluorometric method) and the platelet aggregation test (aggregation of cuvettes in a four-channel platelet aggregometer) were also conducted. The rats’ renal function was evaluated by measuring serum urea and creatinine. Results: Data showed that coffee intake had no effect on the hematological parameters. Fasting glucose and HbA1c dosage were significantly higher in diabetic animals compared to non-diabetic animals (confirmed the effectiveness of inducing and maintaining diabetic status). Results showed that coffee reduced AGE formation and platelet aggregation in our animal model, not altering the animals’ renal function. Conclusions: These results suggest beneficial effects on vasculopathy, a common complication in diabetic patients.http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/711coffea arabica l.diabetes mellitusend products of advanced glycationaminoguanidineplatelet aggregation |
| spellingShingle | Alessandra dos Santos Danziger Silvério Rosemary Gualberto Fonseca Alvarenga Pereira Stella Maris da Silveira Duarte Sônia Aparecida Figueiredo Fernanda Borges de Araújo Paula Tomaz Henrique Araújo Cláudia de Souza Ferreira Bruno César Corrêa Salles André Luiz Machado Viana Eric Batista Ferreira Maria Rita Rodrigues Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats Revista de Ciências Farmacêuticas Básica e Aplicada coffea arabica l. diabetes mellitus end products of advanced glycation aminoguanidine platelet aggregation |
| title | Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats |
| title_full | Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats |
| title_fullStr | Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats |
| title_full_unstemmed | Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats |
| title_short | Coffee intake (Coffea arabica L.) reduces advanced glycation end product (AGEs) formation and platelet aggregation in diabetic rats |
| title_sort | coffee intake coffea arabica l reduces advanced glycation end product ages formation and platelet aggregation in diabetic rats |
| topic | coffea arabica l. diabetes mellitus end products of advanced glycation aminoguanidine platelet aggregation |
| url | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/711 |
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