Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags
Gap-enhanced Raman tags (GERTs) were widely used in cell or biological tissue imaging due to their narrow spectral linewidth, weak photobleaching effect, and low biological matrix interference. Here, we reported a new kind of graphene-wrapped, petal-like, gap-enhanced Raman tags (GP-GERTs). The 4-Ni...
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MDPI AG
2022-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/10/1626 |
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| author | Ming Chen Bin Wang Jingfan Wang Hongliang Liu Zhixiang Chen Xiaoxuan Xu Xing Zhao |
| author_facet | Ming Chen Bin Wang Jingfan Wang Hongliang Liu Zhixiang Chen Xiaoxuan Xu Xing Zhao |
| author_sort | Ming Chen |
| collection | DOAJ |
| description | Gap-enhanced Raman tags (GERTs) were widely used in cell or biological tissue imaging due to their narrow spectral linewidth, weak photobleaching effect, and low biological matrix interference. Here, we reported a new kind of graphene-wrapped, petal-like, gap-enhanced Raman tags (GP-GERTs). The 4-Nitrobenzenethiol (4-NBT) Raman reporters were embedded in the petal-like nanogap, and graphene was wrapped on the surface of the petal-like, gap-enhanced Raman tags. Finite-difference time-domain (FDTD) simulations and Raman experimental studies jointly reveal the Raman enhancement mechanism of graphene. The <i>SERS</i> enhancement of GP-GERTs is jointly determined by the petal-like “interstitial hotspots” and electron transfer between graphene and 4-NBT molecules, and the total Raman enhancement factor (<i>EF</i>) can reach 10<sup>10</sup>. Mesoporous silica was grown on the surface of GP-GERTs by tetraethyl orthosilicate hydrolysis to obtain Raman tags of MS-GP-GERTs. Raman tag stability experiments showed that: MS-GP-GERTs not only can maintain the signal stability in aqueous solutions of different pH values (from 3 to 12) and simulated the physiological environment (up to 72 h), but it can also stably enhance the signal of different Raman molecules. These highly stable, high-signal-intensity nanotags show great potential for <i>SERS</i>-based bioimaging and multicolor imaging. |
| first_indexed | 2024-03-10T03:17:33Z |
| format | Article |
| id | doaj.art-6bb940ad0439499383ddfb169b327baf |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:17:33Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-6bb940ad0439499383ddfb169b327baf2023-11-23T12:25:33ZengMDPI AGNanomaterials2079-49912022-05-011210162610.3390/nano12101626Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman TagsMing Chen0Bin Wang1Jingfan Wang2Hongliang Liu3Zhixiang Chen4Xiaoxuan Xu5Xing Zhao6Tianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, Institute of Modern Optics, Nankai University, Tianjin 300350, ChinaCollege of Artificial Intelligence, Nankai University, Tianjin 300350, ChinaTianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, Institute of Modern Optics, Nankai University, Tianjin 300350, ChinaTianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, Institute of Modern Optics, Nankai University, Tianjin 300350, ChinaTianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, Institute of Modern Optics, Nankai University, Tianjin 300350, ChinaCollege of Artificial Intelligence, Nankai University, Tianjin 300350, ChinaTianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, Institute of Modern Optics, Nankai University, Tianjin 300350, ChinaGap-enhanced Raman tags (GERTs) were widely used in cell or biological tissue imaging due to their narrow spectral linewidth, weak photobleaching effect, and low biological matrix interference. Here, we reported a new kind of graphene-wrapped, petal-like, gap-enhanced Raman tags (GP-GERTs). The 4-Nitrobenzenethiol (4-NBT) Raman reporters were embedded in the petal-like nanogap, and graphene was wrapped on the surface of the petal-like, gap-enhanced Raman tags. Finite-difference time-domain (FDTD) simulations and Raman experimental studies jointly reveal the Raman enhancement mechanism of graphene. The <i>SERS</i> enhancement of GP-GERTs is jointly determined by the petal-like “interstitial hotspots” and electron transfer between graphene and 4-NBT molecules, and the total Raman enhancement factor (<i>EF</i>) can reach 10<sup>10</sup>. Mesoporous silica was grown on the surface of GP-GERTs by tetraethyl orthosilicate hydrolysis to obtain Raman tags of MS-GP-GERTs. Raman tag stability experiments showed that: MS-GP-GERTs not only can maintain the signal stability in aqueous solutions of different pH values (from 3 to 12) and simulated the physiological environment (up to 72 h), but it can also stably enhance the signal of different Raman molecules. These highly stable, high-signal-intensity nanotags show great potential for <i>SERS</i>-based bioimaging and multicolor imaging.https://www.mdpi.com/2079-4991/12/10/1626gap-enhanced Raman tagsgrapheneRaman-enhanced mechanismstability |
| spellingShingle | Ming Chen Bin Wang Jingfan Wang Hongliang Liu Zhixiang Chen Xiaoxuan Xu Xing Zhao Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags Nanomaterials gap-enhanced Raman tags graphene Raman-enhanced mechanism stability |
| title | Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags |
| title_full | Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags |
| title_fullStr | Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags |
| title_full_unstemmed | Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags |
| title_short | Highly Stable, Graphene-Wrapped, Petal-like, Gap-Enhanced Raman Tags |
| title_sort | highly stable graphene wrapped petal like gap enhanced raman tags |
| topic | gap-enhanced Raman tags graphene Raman-enhanced mechanism stability |
| url | https://www.mdpi.com/2079-4991/12/10/1626 |
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