Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS
Saponins in the <i>Camellia sinensis</i> seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to dev...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/20/3794 |
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| author | Xuejin Wu Lingyan Jia Jiafan Wu Yawen Liu Hyunuk Kang Xiaobo Liu Pan Li Puming He Youying Tu Bo Li |
| author_facet | Xuejin Wu Lingyan Jia Jiafan Wu Yawen Liu Hyunuk Kang Xiaobo Liu Pan Li Puming He Youying Tu Bo Li |
| author_sort | Xuejin Wu |
| collection | DOAJ |
| description | Saponins in the <i>Camellia sinensis</i> seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to develop a novel method to tentatively identify and quantify saponins in <i>C. sinensis</i> seeds by ultra-high-performance liquid chromatography coupled with photo-diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS), and compare it with the classic vanillin-sulfuric acid assay. Fifty-one triterpene saponins, including six potentially new compounds, were simultaneously detected by UPLC-PDA-MS/MS, and their chemical structures were speculated according to the retention behavior and fragmentation pattern. The total saponin content in the crude extract and the purified saponin fraction of <i>C. sinensis</i> seeds were quantified to be 19.57 ± 0.05% (wt %) and 41.68 ± 0.09% (wt %) respectively by UPLC-PDA at 210 nm, while the corresponding values were determined to be 43.11 ± 3.17% (wt %) and 56.60 ± 5.79% (wt %) respectively by the vanillin-sulfuric acid assay. The developed UPLC-PDA -MS/MS method could determine specified saponins, and is more reliable for quantifying the <i>C. sinensis</i> seed saponins than the classic spectrophotometric method. It is of great significance for the future investigations and applications of these saponins. |
| first_indexed | 2024-12-12T06:20:54Z |
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| last_indexed | 2024-12-12T06:20:54Z |
| publishDate | 2019-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6bba023b86cd40cca1f015e6c3e4ea082022-12-22T00:34:54ZengMDPI AGMolecules1420-30492019-10-012420379410.3390/molecules24203794molecules24203794Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MSXuejin Wu0Lingyan Jia1Jiafan Wu2Yawen Liu3Hyunuk Kang4Xiaobo Liu5Pan Li6Puming He7Youying Tu8Bo Li9Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaSaponins in the <i>Camellia sinensis</i> seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to develop a novel method to tentatively identify and quantify saponins in <i>C. sinensis</i> seeds by ultra-high-performance liquid chromatography coupled with photo-diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS), and compare it with the classic vanillin-sulfuric acid assay. Fifty-one triterpene saponins, including six potentially new compounds, were simultaneously detected by UPLC-PDA-MS/MS, and their chemical structures were speculated according to the retention behavior and fragmentation pattern. The total saponin content in the crude extract and the purified saponin fraction of <i>C. sinensis</i> seeds were quantified to be 19.57 ± 0.05% (wt %) and 41.68 ± 0.09% (wt %) respectively by UPLC-PDA at 210 nm, while the corresponding values were determined to be 43.11 ± 3.17% (wt %) and 56.60 ± 5.79% (wt %) respectively by the vanillin-sulfuric acid assay. The developed UPLC-PDA -MS/MS method could determine specified saponins, and is more reliable for quantifying the <i>C. sinensis</i> seed saponins than the classic spectrophotometric method. It is of great significance for the future investigations and applications of these saponins.https://www.mdpi.com/1420-3049/24/20/3794saponin<i>camellia sinensis</i> seeduplc-pda-qtof-ms/msidentificationquantitationvanillin-sulfuric acid |
| spellingShingle | Xuejin Wu Lingyan Jia Jiafan Wu Yawen Liu Hyunuk Kang Xiaobo Liu Pan Li Puming He Youying Tu Bo Li Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS Molecules saponin <i>camellia sinensis</i> seed uplc-pda-qtof-ms/ms identification quantitation vanillin-sulfuric acid |
| title | Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS |
| title_full | Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS |
| title_fullStr | Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS |
| title_full_unstemmed | Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS |
| title_short | Simultaneous Determination and Quantification of Triterpene Saponins from <i>Camellia sinensis</i> Seeds Using UPLC-PDA-QTOF-MS/MS |
| title_sort | simultaneous determination and quantification of triterpene saponins from i camellia sinensis i seeds using uplc pda qtof ms ms |
| topic | saponin <i>camellia sinensis</i> seed uplc-pda-qtof-ms/ms identification quantitation vanillin-sulfuric acid |
| url | https://www.mdpi.com/1420-3049/24/20/3794 |
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