Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification
Abstract Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label‐free methods without (bio)‐chemical markers or surface‐engineered receptors remains challenging. Here, we exploit the natural cellular response to mild t...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-08-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202200459 |
| _version_ | 1798036710447120384 |
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| author | Derick Yongabi Mehran Khorshid Patricia Losada‐Pérez Soroush Bakhshi Sichani Stijn Jooken Wouter Stilman Florian Theßeling Tobie Martens Toon Van Thillo Kevin Verstrepen Peter Dedecker Pieter Vanden Berghe Minne Paul Lettinga Carmen Bartic Peter Lieberzeit Michael J. Schöning Ronald Thoelen Marc Fransen Michael Wübbenhorst Patrick Wagner |
| author_facet | Derick Yongabi Mehran Khorshid Patricia Losada‐Pérez Soroush Bakhshi Sichani Stijn Jooken Wouter Stilman Florian Theßeling Tobie Martens Toon Van Thillo Kevin Verstrepen Peter Dedecker Pieter Vanden Berghe Minne Paul Lettinga Carmen Bartic Peter Lieberzeit Michael J. Schöning Ronald Thoelen Marc Fransen Michael Wübbenhorst Patrick Wagner |
| author_sort | Derick Yongabi |
| collection | DOAJ |
| description | Abstract Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label‐free methods without (bio)‐chemical markers or surface‐engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label‐ and receptor‐free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell‐detachment with sharply defined time‐patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell‐type‐specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic‐type oscillations are observed during detachment of mammalian and yeast‐cell ensembles, providing additional cell‐specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs. |
| first_indexed | 2024-04-11T21:16:41Z |
| format | Article |
| id | doaj.art-6bba61fb46ba42bfbfc13def213784b6 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-11T21:16:41Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-6bba61fb46ba42bfbfc13def213784b62022-12-22T04:02:47ZengWileyAdvanced Science2198-38442022-08-01924n/an/a10.1002/advs.202200459Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and IdentificationDerick Yongabi0Mehran Khorshid1Patricia Losada‐Pérez2Soroush Bakhshi Sichani3Stijn Jooken4Wouter Stilman5Florian Theßeling6Tobie Martens7Toon Van Thillo8Kevin Verstrepen9Peter Dedecker10Pieter Vanden Berghe11Minne Paul Lettinga12Carmen Bartic13Peter Lieberzeit14Michael J. Schöning15Ronald Thoelen16Marc Fransen17Michael Wübbenhorst18Patrick Wagner19Laboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumFaculté des Sciences Experimental Soft Matter and Thermal Physics (EST) Université Libre de Bruxelles Boulevard du Triomphe ACC.2 Brussels B‐1050 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Systems Biology VIB Center for Microbiology Department of Microbial and Molecular Systems KU Leuven Gaston Geenslaan 1 Heverlee B‐3001 BelgiumLaboratory for Enteric Neuroscience (LENS) Department of Chronic Diseases Metabolism and Ageing KU Leuven Herestraat 49 Leuven B‐3000 BelgiumBiochemistry Molecular and Structural Biology KU Leuven Celestijnenlaan 200 G Leuven B‐3001 BelgiumLaboratory for Systems Biology VIB Center for Microbiology Department of Microbial and Molecular Systems KU Leuven Gaston Geenslaan 1 Heverlee B‐3001 BelgiumBiochemistry Molecular and Structural Biology KU Leuven Celestijnenlaan 200 G Leuven B‐3001 BelgiumLaboratory for Enteric Neuroscience (LENS) Department of Chronic Diseases Metabolism and Ageing KU Leuven Herestraat 49 Leuven B‐3000 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumFaculty of Chemistry Department of Physical Chemistry University of Vienna Währinger, Straße 38 Vienna A‐1090 AustriaInstitute of Nano‐ and Biotechnologies INB Aachen University of Applied Sciences Heinrich‐Mußmann‐Straße 1 D‐52428 Jülich GermanyInstitute for Materials Research Hasselt University Wetenschapspark 1 Diepenbeek B‐3590 BelgiumLaboratory of Peroxisome Biology and Intracellular Communication Department of Cellular and Molecular Medicine KU Leuven Herestraat 49 Leuven B‐3000 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumLaboratory for Soft Matter and Biophysics Department of Physics and Astronomy KU Leuven Celestijnenlaan 200 D Leuven B‐3001 BelgiumAbstract Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label‐free methods without (bio)‐chemical markers or surface‐engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label‐ and receptor‐free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell‐detachment with sharply defined time‐patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell‐type‐specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic‐type oscillations are observed during detachment of mammalian and yeast‐cell ensembles, providing additional cell‐specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs.https://doi.org/10.1002/advs.202200459cancer therapycell characterizationcell detectionglycolytic oscillationsheat‐transfer methodmetabolic activity |
| spellingShingle | Derick Yongabi Mehran Khorshid Patricia Losada‐Pérez Soroush Bakhshi Sichani Stijn Jooken Wouter Stilman Florian Theßeling Tobie Martens Toon Van Thillo Kevin Verstrepen Peter Dedecker Pieter Vanden Berghe Minne Paul Lettinga Carmen Bartic Peter Lieberzeit Michael J. Schöning Ronald Thoelen Marc Fransen Michael Wübbenhorst Patrick Wagner Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification Advanced Science cancer therapy cell characterization cell detection glycolytic oscillations heat‐transfer method metabolic activity |
| title | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification |
| title_full | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification |
| title_fullStr | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification |
| title_full_unstemmed | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification |
| title_short | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification |
| title_sort | synchronized spontaneous and oscillatory detachment of eukaryotic cells a new tool for cell characterization and identification |
| topic | cancer therapy cell characterization cell detection glycolytic oscillations heat‐transfer method metabolic activity |
| url | https://doi.org/10.1002/advs.202200459 |
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