Prime Editing: An All-Rounder for Genome Editing
Prime editing (PE), as a “search-and-replace” genome editing technology, has shown the attractive potential of versatile genome editing ability, which is, in principle, currently superior to other well-established genome-editing technologies in the all-in-one operation scope. However, essential tech...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/9862 |
| _version_ | 1797495143547273216 |
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| author | Chenyu Lu Jingyu Kuang Tong Shao Sisi Xie Ming Li Lingyun Zhu Lvyun Zhu |
| author_facet | Chenyu Lu Jingyu Kuang Tong Shao Sisi Xie Ming Li Lingyun Zhu Lvyun Zhu |
| author_sort | Chenyu Lu |
| collection | DOAJ |
| description | Prime editing (PE), as a “search-and-replace” genome editing technology, has shown the attractive potential of versatile genome editing ability, which is, in principle, currently superior to other well-established genome-editing technologies in the all-in-one operation scope. However, essential technological solutions of PE technology, such as the improvement of genome editing efficiency, the inhibition of potential off-targets and intended edits accounting for unexpected side-effects, and the development of effective delivery systems, are necessary to broaden its application. Since the advent of PE, many optimizations have been performed on PE systems to improve their performance, resulting in bright prospects for application in many fields. This review briefly discusses the development of PE technology, including its functional principle, noteworthy barriers restraining its application, current efforts in technical optimization, and its application directions and potential risks. This review may provide a concise and informative insight into the burgeoning field of PE, highlight the exciting prospects for this powerful tool, and provide clues for questions that may propel the field forward. |
| first_indexed | 2024-03-10T01:44:14Z |
| format | Article |
| id | doaj.art-6bbca04157ad4951b60789ee0a3ea4fd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:44:14Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6bbca04157ad4951b60789ee0a3ea4fd2023-11-23T13:18:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317986210.3390/ijms23179862Prime Editing: An All-Rounder for Genome EditingChenyu Lu0Jingyu Kuang1Tong Shao2Sisi Xie3Ming Li4Lingyun Zhu5Lvyun Zhu6Department of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaDepartment of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, ChinaPrime editing (PE), as a “search-and-replace” genome editing technology, has shown the attractive potential of versatile genome editing ability, which is, in principle, currently superior to other well-established genome-editing technologies in the all-in-one operation scope. However, essential technological solutions of PE technology, such as the improvement of genome editing efficiency, the inhibition of potential off-targets and intended edits accounting for unexpected side-effects, and the development of effective delivery systems, are necessary to broaden its application. Since the advent of PE, many optimizations have been performed on PE systems to improve their performance, resulting in bright prospects for application in many fields. This review briefly discusses the development of PE technology, including its functional principle, noteworthy barriers restraining its application, current efforts in technical optimization, and its application directions and potential risks. This review may provide a concise and informative insight into the burgeoning field of PE, highlight the exciting prospects for this powerful tool, and provide clues for questions that may propel the field forward.https://www.mdpi.com/1422-0067/23/17/9862prime editinggenome editingtechnical optimizationapplications |
| spellingShingle | Chenyu Lu Jingyu Kuang Tong Shao Sisi Xie Ming Li Lingyun Zhu Lvyun Zhu Prime Editing: An All-Rounder for Genome Editing International Journal of Molecular Sciences prime editing genome editing technical optimization applications |
| title | Prime Editing: An All-Rounder for Genome Editing |
| title_full | Prime Editing: An All-Rounder for Genome Editing |
| title_fullStr | Prime Editing: An All-Rounder for Genome Editing |
| title_full_unstemmed | Prime Editing: An All-Rounder for Genome Editing |
| title_short | Prime Editing: An All-Rounder for Genome Editing |
| title_sort | prime editing an all rounder for genome editing |
| topic | prime editing genome editing technical optimization applications |
| url | https://www.mdpi.com/1422-0067/23/17/9862 |
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