Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.

Molecular taxonomy and evolution of nematodes have been recently the focus of several studies. Mitochondrial sequences were proposed as an alternative for precise identification of Meloidogyne species, to study intraspecific variability and to follow maternal lineages. We characterized the mitochond...

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Main Authors: Laura Evangelina García, M Virginia Sánchez-Puerta
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4370701?pdf=render
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author Laura Evangelina García
M Virginia Sánchez-Puerta
author_facet Laura Evangelina García
M Virginia Sánchez-Puerta
author_sort Laura Evangelina García
collection DOAJ
description Molecular taxonomy and evolution of nematodes have been recently the focus of several studies. Mitochondrial sequences were proposed as an alternative for precise identification of Meloidogyne species, to study intraspecific variability and to follow maternal lineages. We characterized the mitochondrial genomes (mtDNAs) of the root knot nematodes M. floridensis, M. hapla and M. incognita. These were AT rich (81-83%) and highly compact, encoding 12 proteins, 2 rRNAs, and 22 tRNAs. Comparisons with published mtDNAs of M. chitwoodi, M. incognita (another strain) and M. graminicola revealed that they share protein and rRNA gene order but differ in the order of tRNAs. The mtDNAs of M. floridensis and M. incognita were strikingly similar (97-100% identity for all coding regions). In contrast, M. floridensis, M. chitwoodi, M. hapla and M. graminicola showed 65-84% nucleotide identity for coding regions. Variable mitochondrial sequences are potentially useful for evolutionary and taxonomic studies. We developed a molecular taxonomic marker by sequencing a highly-variable ~2 kb mitochondrial region, nad5-cox1, from 36 populations of root-knot nematodes to elucidate relationships within the genus Meloidogyne. Isolates of five species formed monophyletic groups and showed little intraspecific variability. We also present a thorough analysis of the mitochondrial region cox2-rrnS. Phylogenies based on either mitochondrial region had good discrimination power but could not discriminate between M. arenaria, M. incognita and M. floridensis.
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spelling doaj.art-6bbcd6813a0e4056b3a7fb8febabe8c92022-12-21T22:24:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012114210.1371/journal.pone.0121142Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.Laura Evangelina GarcíaM Virginia Sánchez-PuertaMolecular taxonomy and evolution of nematodes have been recently the focus of several studies. Mitochondrial sequences were proposed as an alternative for precise identification of Meloidogyne species, to study intraspecific variability and to follow maternal lineages. We characterized the mitochondrial genomes (mtDNAs) of the root knot nematodes M. floridensis, M. hapla and M. incognita. These were AT rich (81-83%) and highly compact, encoding 12 proteins, 2 rRNAs, and 22 tRNAs. Comparisons with published mtDNAs of M. chitwoodi, M. incognita (another strain) and M. graminicola revealed that they share protein and rRNA gene order but differ in the order of tRNAs. The mtDNAs of M. floridensis and M. incognita were strikingly similar (97-100% identity for all coding regions). In contrast, M. floridensis, M. chitwoodi, M. hapla and M. graminicola showed 65-84% nucleotide identity for coding regions. Variable mitochondrial sequences are potentially useful for evolutionary and taxonomic studies. We developed a molecular taxonomic marker by sequencing a highly-variable ~2 kb mitochondrial region, nad5-cox1, from 36 populations of root-knot nematodes to elucidate relationships within the genus Meloidogyne. Isolates of five species formed monophyletic groups and showed little intraspecific variability. We also present a thorough analysis of the mitochondrial region cox2-rrnS. Phylogenies based on either mitochondrial region had good discrimination power but could not discriminate between M. arenaria, M. incognita and M. floridensis.http://europepmc.org/articles/PMC4370701?pdf=render
spellingShingle Laura Evangelina García
M Virginia Sánchez-Puerta
Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
PLoS ONE
title Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
title_full Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
title_fullStr Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
title_full_unstemmed Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
title_short Comparative and evolutionary analyses of Meloidogyne spp. Based on mitochondrial genome sequences.
title_sort comparative and evolutionary analyses of meloidogyne spp based on mitochondrial genome sequences
url http://europepmc.org/articles/PMC4370701?pdf=render
work_keys_str_mv AT lauraevangelinagarcia comparativeandevolutionaryanalysesofmeloidogynesppbasedonmitochondrialgenomesequences
AT mvirginiasanchezpuerta comparativeandevolutionaryanalysesofmeloidogynesppbasedonmitochondrialgenomesequences