MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells
The direct shedding of extracellular vesicles (EVs) from the plasma membrane is a recognized fundamental method for the intercellular transfer of properties in both physiological and pathological conditions. EVs are classified according to origin, biogenesis, size, content, surface markers, and/or f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/12/6149 |
| _version_ | 1797490266755563520 |
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| author | Carlo Zambonin |
| author_facet | Carlo Zambonin |
| author_sort | Carlo Zambonin |
| collection | DOAJ |
| description | The direct shedding of extracellular vesicles (EVs) from the plasma membrane is a recognized fundamental method for the intercellular transfer of properties in both physiological and pathological conditions. EVs are classified according to origin, biogenesis, size, content, surface markers, and/or functional properties, and contain various bioactive molecules depending on the physiological state and the type of the cells of origin including lipids, nucleic acids, and proteins. The presence of tumor-derived EVs in body fluids such as blood, ascites, urine, and saliva, together with the important role played in the tumor microenvironment where they intervene at different levels from oncogenesis to metastasis, make EVs a priority target for cancer studies. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can play a leading role in the analysis and characterization of EVs and their load due to its intrinsic advantages such as high throughput, low sample consumption, speed, the cost-effectiveness of the analysis, and the ease of use. This work reviews the main MALDI-TOF applications for the analysis and characterization of extracellular vesicles in the tumor field. |
| first_indexed | 2024-03-10T00:29:32Z |
| format | Article |
| id | doaj.art-6bbe2a426ac74c95870a78c63f695eef |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T00:29:32Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-6bbe2a426ac74c95870a78c63f695eef2023-11-23T15:28:37ZengMDPI AGApplied Sciences2076-34172022-06-011212614910.3390/app12126149MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer CellsCarlo Zambonin0Dipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Via E. Orabona 4, 70124 Bari, ItalyThe direct shedding of extracellular vesicles (EVs) from the plasma membrane is a recognized fundamental method for the intercellular transfer of properties in both physiological and pathological conditions. EVs are classified according to origin, biogenesis, size, content, surface markers, and/or functional properties, and contain various bioactive molecules depending on the physiological state and the type of the cells of origin including lipids, nucleic acids, and proteins. The presence of tumor-derived EVs in body fluids such as blood, ascites, urine, and saliva, together with the important role played in the tumor microenvironment where they intervene at different levels from oncogenesis to metastasis, make EVs a priority target for cancer studies. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can play a leading role in the analysis and characterization of EVs and their load due to its intrinsic advantages such as high throughput, low sample consumption, speed, the cost-effectiveness of the analysis, and the ease of use. This work reviews the main MALDI-TOF applications for the analysis and characterization of extracellular vesicles in the tumor field.https://www.mdpi.com/2076-3417/12/12/6149MALDI-TOF MScancerextracellular vesiclesexosomesdiagnosis |
| spellingShingle | Carlo Zambonin MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells Applied Sciences MALDI-TOF MS cancer extracellular vesicles exosomes diagnosis |
| title | MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells |
| title_full | MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells |
| title_fullStr | MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells |
| title_full_unstemmed | MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells |
| title_short | MALDI-TOF/MS Analysis of Extracellular Vesicles Released by Cancer Cells |
| title_sort | maldi tof ms analysis of extracellular vesicles released by cancer cells |
| topic | MALDI-TOF MS cancer extracellular vesicles exosomes diagnosis |
| url | https://www.mdpi.com/2076-3417/12/12/6149 |
| work_keys_str_mv | AT carlozambonin malditofmsanalysisofextracellularvesiclesreleasedbycancercells |