Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia
Abstract Background Mounting experimental evidence has shown that the gut microbiota plays a significant role in the pathogenesis of mastitis, and clinical investigations have found that the occurrence of mastitis is correlated with ruminal dysbiosis. However, the underlying mechanism by which the r...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40168-022-01402-z |
| _version_ | 1828174621543759872 |
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| author | Caijun Zhao Xiaoyu Hu Lijuan Bao Keyi Wu Yihong Zhao Kaihe Xiang Shuang Li Ying Wang Min Qiu Lianjun Feng Xiangyue Meng Naisheng Zhang Yunhe Fu |
| author_facet | Caijun Zhao Xiaoyu Hu Lijuan Bao Keyi Wu Yihong Zhao Kaihe Xiang Shuang Li Ying Wang Min Qiu Lianjun Feng Xiangyue Meng Naisheng Zhang Yunhe Fu |
| author_sort | Caijun Zhao |
| collection | DOAJ |
| description | Abstract Background Mounting experimental evidence has shown that the gut microbiota plays a significant role in the pathogenesis of mastitis, and clinical investigations have found that the occurrence of mastitis is correlated with ruminal dysbiosis. However, the underlying mechanism by which the ruminal microbiota participates in the development of mastitis remains unknown. Results In the present study, we found that cows with clinical mastitis had marked systemic inflammation, which was associated with significant ruminal dysbiosis, especially enriched Proteobacteria in the rumen. Ruminal microbiota transplantation from mastitis cows (M-RMT) to mice induced mastitis symptoms in recipient mice along with increased mammary proinflammatory signature activation of the TLR4-cGAS-STING-NF-κB/NLRP3 pathways. M-RMT also induced mucosal inflammation and impaired intestinal barrier integrity, leading to increased endotoxemia and systemic inflammation. Moreover, we showed that M-RMT mirrored ruminal microbiota disruption in the gut of recipient mice, as evidenced by enriched Proteobacteria and similar bacterial functions, which were correlated with most proinflammatory parameters and serum lipopolysaccharide (LPS) levels in mice. Recurrent low-grade LPS treatment mirrored gut dysbiosis-induced endotoxemia and caused severe mastitis in mice. Furthermore, we found that gut dysbiosis-derived LPS reduced host alkaline phosphatase activity by activating neuraminidase (Neu), which facilitates low-grade LPS exposure and E. coli-induced mastitis in mice. Conversely, treatment with calf intestinal alkaline phosphatase or the Neu inhibitor zanamivir alleviated low-grade LPS exposure and E. coli-induced mastitis in mice. Conclusions Our results suggest that ruminal dysbiosis-derived low-grade endotoxemia can cause mastitis and aggravate pathogen-induced mastitis by impairing host anti-inflammatory enzymes, which implies that regulating the ruminal or gut microbiota to prevent low-grade systemic inflammation is a potential strategy for mastitis intervention. Video Abstract |
| first_indexed | 2024-04-12T04:15:41Z |
| format | Article |
| id | doaj.art-6bbe7ca475b84908ab46db7ffee9288a |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-04-12T04:15:41Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-6bbe7ca475b84908ab46db7ffee9288a2022-12-22T03:48:25ZengBMCMicrobiome2049-26182022-12-0110112210.1186/s40168-022-01402-zGut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemiaCaijun Zhao0Xiaoyu Hu1Lijuan Bao2Keyi Wu3Yihong Zhao4Kaihe Xiang5Shuang Li6Ying Wang7Min Qiu8Lianjun Feng9Xiangyue Meng10Naisheng Zhang11Yunhe Fu12Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Breast Center, West China Hospital, Sichuan UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin UniversityAbstract Background Mounting experimental evidence has shown that the gut microbiota plays a significant role in the pathogenesis of mastitis, and clinical investigations have found that the occurrence of mastitis is correlated with ruminal dysbiosis. However, the underlying mechanism by which the ruminal microbiota participates in the development of mastitis remains unknown. Results In the present study, we found that cows with clinical mastitis had marked systemic inflammation, which was associated with significant ruminal dysbiosis, especially enriched Proteobacteria in the rumen. Ruminal microbiota transplantation from mastitis cows (M-RMT) to mice induced mastitis symptoms in recipient mice along with increased mammary proinflammatory signature activation of the TLR4-cGAS-STING-NF-κB/NLRP3 pathways. M-RMT also induced mucosal inflammation and impaired intestinal barrier integrity, leading to increased endotoxemia and systemic inflammation. Moreover, we showed that M-RMT mirrored ruminal microbiota disruption in the gut of recipient mice, as evidenced by enriched Proteobacteria and similar bacterial functions, which were correlated with most proinflammatory parameters and serum lipopolysaccharide (LPS) levels in mice. Recurrent low-grade LPS treatment mirrored gut dysbiosis-induced endotoxemia and caused severe mastitis in mice. Furthermore, we found that gut dysbiosis-derived LPS reduced host alkaline phosphatase activity by activating neuraminidase (Neu), which facilitates low-grade LPS exposure and E. coli-induced mastitis in mice. Conversely, treatment with calf intestinal alkaline phosphatase or the Neu inhibitor zanamivir alleviated low-grade LPS exposure and E. coli-induced mastitis in mice. Conclusions Our results suggest that ruminal dysbiosis-derived low-grade endotoxemia can cause mastitis and aggravate pathogen-induced mastitis by impairing host anti-inflammatory enzymes, which implies that regulating the ruminal or gut microbiota to prevent low-grade systemic inflammation is a potential strategy for mastitis intervention. Video Abstracthttps://doi.org/10.1186/s40168-022-01402-zRuminal microbiotaMastitisDysbiosisLPSALPNeu |
| spellingShingle | Caijun Zhao Xiaoyu Hu Lijuan Bao Keyi Wu Yihong Zhao Kaihe Xiang Shuang Li Ying Wang Min Qiu Lianjun Feng Xiangyue Meng Naisheng Zhang Yunhe Fu Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia Microbiome Ruminal microbiota Mastitis Dysbiosis LPS ALP Neu |
| title | Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia |
| title_full | Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia |
| title_fullStr | Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia |
| title_full_unstemmed | Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia |
| title_short | Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia |
| title_sort | gut dysbiosis induces the development of mastitis through a reduction in host anti inflammatory enzyme activity by endotoxemia |
| topic | Ruminal microbiota Mastitis Dysbiosis LPS ALP Neu |
| url | https://doi.org/10.1186/s40168-022-01402-z |
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