Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats
Background: Hydrogen has anti-inflammatory and antioxidant effects and is beneficial to multiple organs. However, its effect on alveolar macrophage (AM) pyroptosis induced by burns is still unclear. The purpose of this research was to study the possible positive effects of hydrogen on burn-induced l...
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MDPI AG
2023-02-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/13/3/377 |
| _version_ | 1797610801181229056 |
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| author | Ning Luo Hua Lin Linlin Zhang Yi Jiang Yue Zhao Qingqing Han Xin Wang Yonghao Yu Chao Qin |
| author_facet | Ning Luo Hua Lin Linlin Zhang Yi Jiang Yue Zhao Qingqing Han Xin Wang Yonghao Yu Chao Qin |
| author_sort | Ning Luo |
| collection | DOAJ |
| description | Background: Hydrogen has anti-inflammatory and antioxidant effects and is beneficial to multiple organs. However, its effect on alveolar macrophage (AM) pyroptosis induced by burns is still unclear. The purpose of this research was to study the possible positive effects of hydrogen on burn-induced lung injury and the effects of hydrogen on AM pyroptosis during acute lung injury (ALI) induced by burns. Methods: In this study, histological changes in rat lungs in vivo were evaluated by micro-CT, and histological changes in isolated lungs were evaluated by hematoxylin and eosin (HE) staining. The expressions of leucine rich repeat (LRR) and pyrin domain (PYD) containing protein 3 (NLRP3), caspase-1 and Gasdermin-D (GSDMD) were analyzed by Western blotting. The expression of GSDMD was measured by immunofluorescence to evaluate the levels of lung inflammation and pyroptosis. The level of inflammation was assessed by enzyme-linked immunosorbent assay (ELISA). Pyroptosis was observed by transmission electron microscopy. Results: We observed that severe burn resulted in increased IL-1β and IL-18, overexpression of NLRP3 and caspase-1 proteins, and pyroptosis in rat lung tissues, as demonstrated by GSDMD overexpression and electron microscopy of AMs. We also observed that hydrogen treatment partially reversed the increase in lung tissue density and reduced pulmonary inflammation. Moreover, hydrogen reduced the HE pathological injury score in the lung tissues of severely burned rats. Hydrogen treatment significantly reduced the contents of IL-1β and IL-18 in the lung tissues and decreased the expression of NLRP3, caspase-1 and GSDMD proteins compared with the burn group. Transmission electron microscopy results also showed that the number of AM membrane pores was significantly reduced in the hydrogen treatment group. Conclusions: The results of this study suggest that hydrogen may protect against ALI induced by burn injury by inhibiting pyroptosis of macrophages via NLRP3. |
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| language | English |
| last_indexed | 2024-03-11T06:20:06Z |
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| series | Journal of Personalized Medicine |
| spelling | doaj.art-6bbee1eab8f8497f843b2fc09a8ebed22023-11-17T12:01:29ZengMDPI AGJournal of Personalized Medicine2075-44262023-02-0113337710.3390/jpm13030377Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in RatsNing Luo0Hua Lin1Linlin Zhang2Yi Jiang3Yue Zhao4Qingqing Han5Xin Wang6Yonghao Yu7Chao Qin8Department of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaDepartment of Anesthesia, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, ChinaBackground: Hydrogen has anti-inflammatory and antioxidant effects and is beneficial to multiple organs. However, its effect on alveolar macrophage (AM) pyroptosis induced by burns is still unclear. The purpose of this research was to study the possible positive effects of hydrogen on burn-induced lung injury and the effects of hydrogen on AM pyroptosis during acute lung injury (ALI) induced by burns. Methods: In this study, histological changes in rat lungs in vivo were evaluated by micro-CT, and histological changes in isolated lungs were evaluated by hematoxylin and eosin (HE) staining. The expressions of leucine rich repeat (LRR) and pyrin domain (PYD) containing protein 3 (NLRP3), caspase-1 and Gasdermin-D (GSDMD) were analyzed by Western blotting. The expression of GSDMD was measured by immunofluorescence to evaluate the levels of lung inflammation and pyroptosis. The level of inflammation was assessed by enzyme-linked immunosorbent assay (ELISA). Pyroptosis was observed by transmission electron microscopy. Results: We observed that severe burn resulted in increased IL-1β and IL-18, overexpression of NLRP3 and caspase-1 proteins, and pyroptosis in rat lung tissues, as demonstrated by GSDMD overexpression and electron microscopy of AMs. We also observed that hydrogen treatment partially reversed the increase in lung tissue density and reduced pulmonary inflammation. Moreover, hydrogen reduced the HE pathological injury score in the lung tissues of severely burned rats. Hydrogen treatment significantly reduced the contents of IL-1β and IL-18 in the lung tissues and decreased the expression of NLRP3, caspase-1 and GSDMD proteins compared with the burn group. Transmission electron microscopy results also showed that the number of AM membrane pores was significantly reduced in the hydrogen treatment group. Conclusions: The results of this study suggest that hydrogen may protect against ALI induced by burn injury by inhibiting pyroptosis of macrophages via NLRP3.https://www.mdpi.com/2075-4426/13/3/377severe burnsacute lung injuryhydrogenpyroptosisNLRP3GSDMD |
| spellingShingle | Ning Luo Hua Lin Linlin Zhang Yi Jiang Yue Zhao Qingqing Han Xin Wang Yonghao Yu Chao Qin Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats Journal of Personalized Medicine severe burns acute lung injury hydrogen pyroptosis NLRP3 GSDMD |
| title | Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats |
| title_full | Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats |
| title_fullStr | Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats |
| title_full_unstemmed | Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats |
| title_short | Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats |
| title_sort | effect of hydrogen on am pyroptosis induced by severe burns in rats |
| topic | severe burns acute lung injury hydrogen pyroptosis NLRP3 GSDMD |
| url | https://www.mdpi.com/2075-4426/13/3/377 |
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