Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves
<i>Cercis glabra</i> is a plant belonging to the legume family, whose flowers and barks are commonly used as food and traditional Chinese medicines. However, its leaves are usually disposed of as wastes. This research comprehensively investigated the bioactive constituents of <i>C....
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8667 |
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| author | Yueyue Lou Ting Xu Huaqiang Cao Qiuyue Zhao Pengpai Zhang Penghua Shu |
| author_facet | Yueyue Lou Ting Xu Huaqiang Cao Qiuyue Zhao Pengpai Zhang Penghua Shu |
| author_sort | Yueyue Lou |
| collection | DOAJ |
| description | <i>Cercis glabra</i> is a plant belonging to the legume family, whose flowers and barks are commonly used as food and traditional Chinese medicines. However, its leaves are usually disposed of as wastes. This research comprehensively investigated the bioactive constituents of <i>C. glabra</i> leaves, and two new phenolic, ceroffesters A-B (<b>1</b>–<b>2</b>) and thirteen known compounds (<b>3</b>–<b>15</b>) were isolated. Their structures were elucidated by spectroscopic methods such as nuclear magnetic resonance (1D NMR and 2D NMR), high-resolution electrospray ionization mass spectra (HR-ESI-MS), optical rotatory dispersion (ORD) and electronic circular dichroism (ECD). All of them were assessed for their antioxidant activities through ABTS, DPPH and PTIO methodologies, and evaluated for inhibitory activities against two enzymes (mushroom tyrosinase and acetylcholinesterase). As a result, compounds <b>3</b>–<b>6</b>, <b>10</b> and <b>13</b> exhibited evident antioxidant activities. Meanwhile, compounds <b>5</b>, <b>10</b> and <b>13</b> showed the most potent tyrosinase inhibitory activities, with IC<sub>50</sub> of 0.64, 0.65 and 0.59 mM, and compared with the positive control of 0.63 mM (kojic acid). In the initial concentration of 1 mg/mL, compounds <b>3</b>, <b>5</b> and <b>6</b> demonstrated moderate inhibitory activities against acetylcholinesterase with 85.27 ± 0.06%, 83.65 ± 0.48% and 82.21 ± 0.09%, respectively, compared with the positive control of 91.17 ± 0.23% (donepezil). These bioactive components could be promising antioxidants, tyrosinase and acetylcholinesterase inhibitors. |
| first_indexed | 2024-03-09T16:03:16Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:03:16Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6bc04ff85cab4d73bf32fce848e292392023-11-24T16:55:22ZengMDPI AGMolecules1420-30492022-12-012724866710.3390/molecules27248667Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> LeavesYueyue Lou0Ting Xu1Huaqiang Cao2Qiuyue Zhao3Pengpai Zhang4Penghua Shu5School of Life Sciences, Henan University, Kaifeng 475004, ChinaSchool of Life Sciences, Henan University, Kaifeng 475004, ChinaSchool of Life Sciences, Henan University, Kaifeng 475004, ChinaFood and Pharmacy College, Xuchang University, 88 Bayi Road, Xuchang 461000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475004, ChinaFood and Pharmacy College, Xuchang University, 88 Bayi Road, Xuchang 461000, China<i>Cercis glabra</i> is a plant belonging to the legume family, whose flowers and barks are commonly used as food and traditional Chinese medicines. However, its leaves are usually disposed of as wastes. This research comprehensively investigated the bioactive constituents of <i>C. glabra</i> leaves, and two new phenolic, ceroffesters A-B (<b>1</b>–<b>2</b>) and thirteen known compounds (<b>3</b>–<b>15</b>) were isolated. Their structures were elucidated by spectroscopic methods such as nuclear magnetic resonance (1D NMR and 2D NMR), high-resolution electrospray ionization mass spectra (HR-ESI-MS), optical rotatory dispersion (ORD) and electronic circular dichroism (ECD). All of them were assessed for their antioxidant activities through ABTS, DPPH and PTIO methodologies, and evaluated for inhibitory activities against two enzymes (mushroom tyrosinase and acetylcholinesterase). As a result, compounds <b>3</b>–<b>6</b>, <b>10</b> and <b>13</b> exhibited evident antioxidant activities. Meanwhile, compounds <b>5</b>, <b>10</b> and <b>13</b> showed the most potent tyrosinase inhibitory activities, with IC<sub>50</sub> of 0.64, 0.65 and 0.59 mM, and compared with the positive control of 0.63 mM (kojic acid). In the initial concentration of 1 mg/mL, compounds <b>3</b>, <b>5</b> and <b>6</b> demonstrated moderate inhibitory activities against acetylcholinesterase with 85.27 ± 0.06%, 83.65 ± 0.48% and 82.21 ± 0.09%, respectively, compared with the positive control of 91.17 ± 0.23% (donepezil). These bioactive components could be promising antioxidants, tyrosinase and acetylcholinesterase inhibitors.https://www.mdpi.com/1420-3049/27/24/8667<i>Cercis glabra</i>flavonoidsantioxidanttyrosinaseacetylcholinesterase |
| spellingShingle | Yueyue Lou Ting Xu Huaqiang Cao Qiuyue Zhao Pengpai Zhang Penghua Shu Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves Molecules <i>Cercis glabra</i> flavonoids antioxidant tyrosinase acetylcholinesterase |
| title | Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves |
| title_full | Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves |
| title_fullStr | Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves |
| title_full_unstemmed | Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves |
| title_short | Natural Antioxidants, Tyrosinase and Acetylcholinesterase Inhibitors from <i>Cercis glabra</i> Leaves |
| title_sort | natural antioxidants tyrosinase and acetylcholinesterase inhibitors from i cercis glabra i leaves |
| topic | <i>Cercis glabra</i> flavonoids antioxidant tyrosinase acetylcholinesterase |
| url | https://www.mdpi.com/1420-3049/27/24/8667 |
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