Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation
Several polyphenol compounds commonly found in plant foods provide health benefits that promote their applications in food and pharmaceutical fields. The co-loading of proteins can improve the biological activity and synergy of combined polyphenols. Herein, multi-spectroscopic techniques and molecul...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622001906 |
| _version_ | 1811242147698966528 |
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| author | Yongfang Ren Tingting Liu He Liu Yihao Zhu Xin Qi Xiaofan Liu Yanna Zhao Yushu Wu Ning Zhang Min Liu |
| author_facet | Yongfang Ren Tingting Liu He Liu Yihao Zhu Xin Qi Xiaofan Liu Yanna Zhao Yushu Wu Ning Zhang Min Liu |
| author_sort | Yongfang Ren |
| collection | DOAJ |
| description | Several polyphenol compounds commonly found in plant foods provide health benefits that promote their applications in food and pharmaceutical fields. The co-loading of proteins can improve the biological activity and synergy of combined polyphenols. Herein, multi-spectroscopic techniques and molecular docking were employed to investigate the interactions of native/heat treated β-lactoglobulin (β-LG/H-β-LG) with (−)-epicatechin gallate (ECG) and/or piceatannol (PIC). Furthermore, the synergistic antioxidant activity and cytotoxicity of ECG and PIC were demonstrated. The loading of β-LG/H-β-LG protected the antioxidant activity of ECG and PIC in the delivery systems and enhanced their cytotoxicity, stability, and bioaccessibility. Compared to the corresponding complexes, the nanoparticles of β-LG/H-β-LG had a greater impact on the functional properties and synergistic effects of ECG and PIC. The experimental results may provide a theoretical basis for the practical application of ECG and PIC co-loaded by H-β-LG, particularly H-β-LG nanoparticles in the food and pharmaceutical industries. |
| first_indexed | 2024-04-12T13:46:45Z |
| format | Article |
| id | doaj.art-6bc265fdfa9245b0931d2cbff95dd28a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-12T13:46:45Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6bc265fdfa9245b0931d2cbff95dd28a2022-12-22T03:30:39ZengElsevierJournal of Functional Foods1756-46462022-07-0194105120Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulationYongfang Ren0Tingting Liu1He Liu2Yihao Zhu3Xin Qi4Xiaofan Liu5Yanna Zhao6Yushu Wu7Ning Zhang8Min Liu9Institute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaSchool of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, ChinaSchool of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaSchool of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, ChinaInstitute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, China; School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China; Corresponding author at: Institute of BioPharmceutical Research, Liaocheng University, Liaocheng 252059, China.Several polyphenol compounds commonly found in plant foods provide health benefits that promote their applications in food and pharmaceutical fields. The co-loading of proteins can improve the biological activity and synergy of combined polyphenols. Herein, multi-spectroscopic techniques and molecular docking were employed to investigate the interactions of native/heat treated β-lactoglobulin (β-LG/H-β-LG) with (−)-epicatechin gallate (ECG) and/or piceatannol (PIC). Furthermore, the synergistic antioxidant activity and cytotoxicity of ECG and PIC were demonstrated. The loading of β-LG/H-β-LG protected the antioxidant activity of ECG and PIC in the delivery systems and enhanced their cytotoxicity, stability, and bioaccessibility. Compared to the corresponding complexes, the nanoparticles of β-LG/H-β-LG had a greater impact on the functional properties and synergistic effects of ECG and PIC. The experimental results may provide a theoretical basis for the practical application of ECG and PIC co-loaded by H-β-LG, particularly H-β-LG nanoparticles in the food and pharmaceutical industries.http://www.sciencedirect.com/science/article/pii/S1756464622001906Polyphenolβ-lactoglobulinHeat treatmentNanoencapsulationSynergistic effect |
| spellingShingle | Yongfang Ren Tingting Liu He Liu Yihao Zhu Xin Qi Xiaofan Liu Yanna Zhao Yushu Wu Ning Zhang Min Liu Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation Journal of Functional Foods Polyphenol β-lactoglobulin Heat treatment Nanoencapsulation Synergistic effect |
| title | Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation |
| title_full | Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation |
| title_fullStr | Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation |
| title_full_unstemmed | Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation |
| title_short | Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation |
| title_sort | functional improvement of epicatechin gallate and piceatannol through combined binding to β lactoglobulin enhanced effect of heat treatment and nanoencapsulation |
| topic | Polyphenol β-lactoglobulin Heat treatment Nanoencapsulation Synergistic effect |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622001906 |
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