Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro
Abstract: Medicinal plants have long been used as an alternative to traditional drugs for the treatment of inflammatory conditions due to the classical side effects and restricted access of various commercially available drugs, such as steroids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs)...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2019-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100612&lng=en&tlng=en |
| _version_ | 1818009012268957696 |
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| author | NORMA V.Q. CARNEIRO HUGO B.F. DA SILVA RAIMON R. DA SILVA TAMIRES C.B. CARNEIRO RYAN S. COSTA ANAQUE O. PIRES CINTIA R. MARQUES EUDES S. VELOZO ADILVA S. CONCEIÇÃO TANIA M.S. DA SILVA TELMA M.G. DA SILVA NEUZA M. ALCÂNTARA-NEVES CAMILA A. FIGUEIREDO |
| author_facet | NORMA V.Q. CARNEIRO HUGO B.F. DA SILVA RAIMON R. DA SILVA TAMIRES C.B. CARNEIRO RYAN S. COSTA ANAQUE O. PIRES CINTIA R. MARQUES EUDES S. VELOZO ADILVA S. CONCEIÇÃO TANIA M.S. DA SILVA TELMA M.G. DA SILVA NEUZA M. ALCÂNTARA-NEVES CAMILA A. FIGUEIREDO |
| author_sort | NORMA V.Q. CARNEIRO |
| collection | DOAJ |
| description | Abstract: Medicinal plants have long been used as an alternative to traditional drugs for the treatment of inflammatory conditions due to the classical side effects and restricted access of various commercially available drugs, such as steroids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs). Sambucus australis is a Brazilian herb that is commonly used to treat inflammatory diseases; however, few studies have examined the use of this species in the treatment of inflammatory conditions. The present study aims to evaluate the potential anti-inflammatory activity of S. australis in vitro. We established spleen cell cultures stimulated with pokeweed mitogen (PWM) to evaluate the production of proinflammatory cytokines, such as IL-4, IL-5, IFN-y, and IL-10 (by ELISA), and the expression of the transcription factor NF-kB (by RT-PCR). In addition, we evaluated the levels of nitric oxide in macrophage cultures and the membrane-stabilizing activity of S. australis methanolic extract (EMSA). Treatment with EMSA at concentrations of 100, 50, 25 and 12.5 µg/ml significantly decreased IL-4 (p<0.001) and IL-5 (p<0.001) levels. Treatment with 100 µg/ml EMSA reduced IFN-у (p<0.001) levels. Moreover, at 100 mg/ml, EMSA also increased IL-10 production and reduced NF-kB expression (p<0.01). In macrophage cultures stimulated with LPS, EMSA decreased nitric oxide levels (p<0.001) at all concentrations tested (100, 50, 25 and 12.5 µg/ml). Additionally, EMSA had a protective effect in the erythrocyte membrane stabilization assay. Taken together, these results suggest that S. australis has anti-inflammatory potential in vitro, characterized by the reduction of both inflammatory cytokines and the expression of NF-kB along with the up-regulation of IL-10. |
| first_indexed | 2024-04-14T05:37:25Z |
| format | Article |
| id | doaj.art-6bc341be22df409fb1cf97813171f695 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-14T05:37:25Z |
| publishDate | 2019-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-6bc341be22df409fb1cf97813171f6952022-12-22T02:09:35ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902019-03-0191110.1590/0001-3765201920170831S0001-37652019000100612Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitroNORMA V.Q. CARNEIROHUGO B.F. DA SILVARAIMON R. DA SILVATAMIRES C.B. CARNEIRORYAN S. COSTAANAQUE O. PIRESCINTIA R. MARQUESEUDES S. VELOZOADILVA S. CONCEIÇÃOTANIA M.S. DA SILVATELMA M.G. DA SILVANEUZA M. ALCÂNTARA-NEVESCAMILA A. FIGUEIREDOAbstract: Medicinal plants have long been used as an alternative to traditional drugs for the treatment of inflammatory conditions due to the classical side effects and restricted access of various commercially available drugs, such as steroids (GCs) and nonsteroidal anti-inflammatory drugs (NSAIDs). Sambucus australis is a Brazilian herb that is commonly used to treat inflammatory diseases; however, few studies have examined the use of this species in the treatment of inflammatory conditions. The present study aims to evaluate the potential anti-inflammatory activity of S. australis in vitro. We established spleen cell cultures stimulated with pokeweed mitogen (PWM) to evaluate the production of proinflammatory cytokines, such as IL-4, IL-5, IFN-y, and IL-10 (by ELISA), and the expression of the transcription factor NF-kB (by RT-PCR). In addition, we evaluated the levels of nitric oxide in macrophage cultures and the membrane-stabilizing activity of S. australis methanolic extract (EMSA). Treatment with EMSA at concentrations of 100, 50, 25 and 12.5 µg/ml significantly decreased IL-4 (p<0.001) and IL-5 (p<0.001) levels. Treatment with 100 µg/ml EMSA reduced IFN-у (p<0.001) levels. Moreover, at 100 mg/ml, EMSA also increased IL-10 production and reduced NF-kB expression (p<0.01). In macrophage cultures stimulated with LPS, EMSA decreased nitric oxide levels (p<0.001) at all concentrations tested (100, 50, 25 and 12.5 µg/ml). Additionally, EMSA had a protective effect in the erythrocyte membrane stabilization assay. Taken together, these results suggest that S. australis has anti-inflammatory potential in vitro, characterized by the reduction of both inflammatory cytokines and the expression of NF-kB along with the up-regulation of IL-10.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100612&lng=en&tlng=enSambucus australiselderberryanti-inflammatoryNF-kB |
| spellingShingle | NORMA V.Q. CARNEIRO HUGO B.F. DA SILVA RAIMON R. DA SILVA TAMIRES C.B. CARNEIRO RYAN S. COSTA ANAQUE O. PIRES CINTIA R. MARQUES EUDES S. VELOZO ADILVA S. CONCEIÇÃO TANIA M.S. DA SILVA TELMA M.G. DA SILVA NEUZA M. ALCÂNTARA-NEVES CAMILA A. FIGUEIREDO Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro Anais da Academia Brasileira de Ciências Sambucus australis elderberry anti-inflammatory NF-kB |
| title | Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro |
| title_full | Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro |
| title_fullStr | Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro |
| title_full_unstemmed | Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro |
| title_short | Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro |
| title_sort | sambucus australis modulates inflammatory response via inhibition of nuclear factor kappa b nf kb in vitro |
| topic | Sambucus australis elderberry anti-inflammatory NF-kB |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100612&lng=en&tlng=en |
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