Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
Several phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and pur...
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MDPI AG
2022-12-01
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Series: | Molecules |
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Online Access: | https://www.mdpi.com/1420-3049/28/1/334 |
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author | Jorge Eugenio Martínez-Vega Erika Villafaña-Estarrón Froylán M. E. Escalante |
author_facet | Jorge Eugenio Martínez-Vega Erika Villafaña-Estarrón Froylán M. E. Escalante |
author_sort | Jorge Eugenio Martínez-Vega |
collection | DOAJ |
description | Several phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and purity of phycocyanin from <i>Arthrospira maxima</i>. We followed three experimental designs to determine the best combination of buffered pH solutions, additives, fresh and lyophilized biomass. We have found that additives such as citric acid and/or disaccharides could be beneficial to the extraction process. We concluded that the biomass–solvent ratio is a determining factor to obtain high extraction and purity ratios with short ultrasonication times. |
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institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-09T09:43:39Z |
publishDate | 2022-12-01 |
publisher | MDPI AG |
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spelling | doaj.art-6bc594edef9f44189f5ce5ebe15342c82023-12-02T00:42:19ZengMDPI AGMolecules1420-30492022-12-0128133410.3390/molecules28010334Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using UltrasonicationJorge Eugenio Martínez-Vega0Erika Villafaña-Estarrón1Froylán M. E. Escalante2Departamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoDepartamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoDirección de Investigación y Desarrollo Tecnológico, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoSeveral phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and purity of phycocyanin from <i>Arthrospira maxima</i>. We followed three experimental designs to determine the best combination of buffered pH solutions, additives, fresh and lyophilized biomass. We have found that additives such as citric acid and/or disaccharides could be beneficial to the extraction process. We concluded that the biomass–solvent ratio is a determining factor to obtain high extraction and purity ratios with short ultrasonication times.https://www.mdpi.com/1420-3049/28/1/334ultrasonicationfreeze–thaw<i>Spirulina</i> |
spellingShingle | Jorge Eugenio Martínez-Vega Erika Villafaña-Estarrón Froylán M. E. Escalante Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication Molecules ultrasonication freeze–thaw <i>Spirulina</i> |
title | Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication |
title_full | Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication |
title_fullStr | Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication |
title_full_unstemmed | Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication |
title_short | Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication |
title_sort | comparative study of the efficiency of additives in the extraction of phycocyanin c from i arthrospira maxima i using ultrasonication |
topic | ultrasonication freeze–thaw <i>Spirulina</i> |
url | https://www.mdpi.com/1420-3049/28/1/334 |
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