Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication

Several phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and pur...

Full description

Bibliographic Details
Main Authors: Jorge Eugenio Martínez-Vega, Erika Villafaña-Estarrón, Froylán M. E. Escalante
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/28/1/334
_version_ 1797431419628158976
author Jorge Eugenio Martínez-Vega
Erika Villafaña-Estarrón
Froylán M. E. Escalante
author_facet Jorge Eugenio Martínez-Vega
Erika Villafaña-Estarrón
Froylán M. E. Escalante
author_sort Jorge Eugenio Martínez-Vega
collection DOAJ
description Several phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and purity of phycocyanin from <i>Arthrospira maxima</i>. We followed three experimental designs to determine the best combination of buffered pH solutions, additives, fresh and lyophilized biomass. We have found that additives such as citric acid and/or disaccharides could be beneficial to the extraction process. We concluded that the biomass–solvent ratio is a determining factor to obtain high extraction and purity ratios with short ultrasonication times.
first_indexed 2024-03-09T09:43:39Z
format Article
id doaj.art-6bc594edef9f44189f5ce5ebe15342c8
institution Directory Open Access Journal
issn 1420-3049
language English
last_indexed 2024-03-09T09:43:39Z
publishDate 2022-12-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj.art-6bc594edef9f44189f5ce5ebe15342c82023-12-02T00:42:19ZengMDPI AGMolecules1420-30492022-12-0128133410.3390/molecules28010334Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using UltrasonicationJorge Eugenio Martínez-Vega0Erika Villafaña-Estarrón1Froylán M. E. Escalante2Departamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoDepartamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoDirección de Investigación y Desarrollo Tecnológico, Universidad Autónoma de Guadalajara, Av. Patria 1201, Lomas del Valle, Zapopan 45129, MexicoSeveral phycocyanin extraction methods have been proposed, however, most of them present economical or productive barriers. One of the most promising methods that has been suggested is ultrasonication. We have analyzed here the effect of operational conditions and additives on the extraction and purity of phycocyanin from <i>Arthrospira maxima</i>. We followed three experimental designs to determine the best combination of buffered pH solutions, additives, fresh and lyophilized biomass. We have found that additives such as citric acid and/or disaccharides could be beneficial to the extraction process. We concluded that the biomass–solvent ratio is a determining factor to obtain high extraction and purity ratios with short ultrasonication times.https://www.mdpi.com/1420-3049/28/1/334ultrasonicationfreeze–thaw<i>Spirulina</i>
spellingShingle Jorge Eugenio Martínez-Vega
Erika Villafaña-Estarrón
Froylán M. E. Escalante
Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
Molecules
ultrasonication
freeze–thaw
<i>Spirulina</i>
title Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
title_full Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
title_fullStr Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
title_full_unstemmed Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
title_short Comparative Study of the Efficiency of Additives in the Extraction of Phycocyanin-C from <i>Arthrospira maxima</i> Using Ultrasonication
title_sort comparative study of the efficiency of additives in the extraction of phycocyanin c from i arthrospira maxima i using ultrasonication
topic ultrasonication
freeze–thaw
<i>Spirulina</i>
url https://www.mdpi.com/1420-3049/28/1/334
work_keys_str_mv AT jorgeeugeniomartinezvega comparativestudyoftheefficiencyofadditivesintheextractionofphycocyanincfromiarthrospiramaximaiusingultrasonication
AT erikavillafanaestarron comparativestudyoftheefficiencyofadditivesintheextractionofphycocyanincfromiarthrospiramaximaiusingultrasonication
AT froylanmeescalante comparativestudyoftheefficiencyofadditivesintheextractionofphycocyanincfromiarthrospiramaximaiusingultrasonication