Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions
Abstract Background Entomopathogens such as nematodes, bacteria and fungi are well recognized for their biocontrol potential. This study was carried out to examine the insecticidal properties of the Heterorhabditis bacteriophora Poinar, Beauveria bassiana Balsamo-Crivelli, Bacillus thuringiensis Ber...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-04-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-022-00537-3 |
| _version_ | 1811302187191500800 |
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| author | Neelam Thakur Preety Tomar Sakshi Sharma Simranjeet Kaur Sushma Sharma Ajar Nath Yadav Abd El-Latif Hesham |
| author_facet | Neelam Thakur Preety Tomar Sakshi Sharma Simranjeet Kaur Sushma Sharma Ajar Nath Yadav Abd El-Latif Hesham |
| author_sort | Neelam Thakur |
| collection | DOAJ |
| description | Abstract Background Entomopathogens such as nematodes, bacteria and fungi are well recognized for their biocontrol potential. This study was carried out to examine the insecticidal properties of the Heterorhabditis bacteriophora Poinar, Beauveria bassiana Balsamo-Crivelli, Bacillus thuringiensis Berliner, individually and in combination against 3rd instar larvae of Spodoptera litura Fabricius (Noctuidae: Lepidoptera) under controlled laboratory and greenhouse conditions at Eternal University, Baru Sahib, Sirmaur, Himachal Pradesh. Results The results demonstrated that the combined applications of the tested entomopathogens resulted in 100% insect mortality under the laboratory conditions. Among the individual concentrations, applications of 200 IJs/ml were noticed highly virulent with (98%) mortality, followed by B. thuringiensis (96%) and then by B. bassiana (92%). However, single treatments were also evaluated that further showed a highest mortality in the target pest by H. bacteriophora, followed by B. thuringiensis. Among the combined treatments by H. bacteriophora plus B. thuringiensis (200 IJs + 1 × 1012 CFU/cm2) more effective caused (100%) mortality were noticed in the laboratory and (28%) under the greenhouse conditions than H. bacteriophora plus B. bassiana (200 IJs + 1 × 1010 conidia/cm2) that caused (100%) mortality and (34%) damage under both, laboratory and greenhouse conditions. Conclusion Laboratory bioassay and greenhouse evaluation tests demonstrated that the combined sprayed treatments showed reliable and fast synergism. This study could be recommended to the farmers to control the pest. |
| first_indexed | 2024-04-13T07:23:32Z |
| format | Article |
| id | doaj.art-6bcae77c22b5456bb16cf6271649be46 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-04-13T07:23:32Z |
| publishDate | 2022-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-6bcae77c22b5456bb16cf6271649be462022-12-22T02:56:32ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-04-0132111010.1186/s41938-022-00537-3Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditionsNeelam Thakur0Preety Tomar1Sakshi Sharma2Simranjeet Kaur3Sushma Sharma4Ajar Nath Yadav5Abd El-Latif Hesham6Department of Zoology, Akal College of Basic Sciences, Eternal UniversityDepartment of Zoology, Akal College of Basic Sciences, Eternal UniversityDepartment of Zoology, Akal College of Basic Sciences, Eternal UniversityDepartment of Zoology, Akal College of Basic Sciences, Eternal UniversityDepartment of Plant Pathology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal UniversityDepartment of Genetics Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal UniversityDepartment of Genetics, Faculty of Agriculture, Beni-Suef UniversityAbstract Background Entomopathogens such as nematodes, bacteria and fungi are well recognized for their biocontrol potential. This study was carried out to examine the insecticidal properties of the Heterorhabditis bacteriophora Poinar, Beauveria bassiana Balsamo-Crivelli, Bacillus thuringiensis Berliner, individually and in combination against 3rd instar larvae of Spodoptera litura Fabricius (Noctuidae: Lepidoptera) under controlled laboratory and greenhouse conditions at Eternal University, Baru Sahib, Sirmaur, Himachal Pradesh. Results The results demonstrated that the combined applications of the tested entomopathogens resulted in 100% insect mortality under the laboratory conditions. Among the individual concentrations, applications of 200 IJs/ml were noticed highly virulent with (98%) mortality, followed by B. thuringiensis (96%) and then by B. bassiana (92%). However, single treatments were also evaluated that further showed a highest mortality in the target pest by H. bacteriophora, followed by B. thuringiensis. Among the combined treatments by H. bacteriophora plus B. thuringiensis (200 IJs + 1 × 1012 CFU/cm2) more effective caused (100%) mortality were noticed in the laboratory and (28%) under the greenhouse conditions than H. bacteriophora plus B. bassiana (200 IJs + 1 × 1010 conidia/cm2) that caused (100%) mortality and (34%) damage under both, laboratory and greenhouse conditions. Conclusion Laboratory bioassay and greenhouse evaluation tests demonstrated that the combined sprayed treatments showed reliable and fast synergism. This study could be recommended to the farmers to control the pest.https://doi.org/10.1186/s41938-022-00537-3Bacillus thuringiensisBeauveria bassianaBio-efficacyHeterorhabditis bacteriophoraSpodoptera litura |
| spellingShingle | Neelam Thakur Preety Tomar Sakshi Sharma Simranjeet Kaur Sushma Sharma Ajar Nath Yadav Abd El-Latif Hesham Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions Egyptian Journal of Biological Pest Control Bacillus thuringiensis Beauveria bassiana Bio-efficacy Heterorhabditis bacteriophora Spodoptera litura |
| title | Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions |
| title_full | Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions |
| title_fullStr | Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions |
| title_full_unstemmed | Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions |
| title_short | Synergistic effect of entomopathogens against Spodoptera litura (Fabricius) under laboratory and greenhouse conditions |
| title_sort | synergistic effect of entomopathogens against spodoptera litura fabricius under laboratory and greenhouse conditions |
| topic | Bacillus thuringiensis Beauveria bassiana Bio-efficacy Heterorhabditis bacteriophora Spodoptera litura |
| url | https://doi.org/10.1186/s41938-022-00537-3 |
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