Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis.
Bone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0213452 |
| _version_ | 1819034103704453120 |
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| author | Deena A Rennerfeldt Joana S Raminhos Samantha M Leff Pristinavae Manning Krystyn J Van Vliet |
| author_facet | Deena A Rennerfeldt Joana S Raminhos Samantha M Leff Pristinavae Manning Krystyn J Van Vliet |
| author_sort | Deena A Rennerfeldt |
| collection | DOAJ |
| description | Bone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the potential for heterogeneity within a single colony forming unit-generated ostensibly from a single mother cell-is less explored but is critical to design of both fundamental studies and cell therapy production. Here we observed BMSC colony formation in real time via time lapsed optical imaging and analysis, to quantify whether and how heterogeneity emerged over multiple cell divisions spanning the duration of a typical colony formation unit assay. These analyses demonstrate that such colonies are neither homogeneous subpopulations of stem cells nor necessarily derived from single originating cells. While the mechanisms for and causes of this intracolony heterogeneity are not understood fully, we further demonstrate that extensive cell-cell contacts do not correlate with senescence, but that media exchange was concurrent with diversification in even the most uniform single-cell-derived colonies. These direct quantitative observations and visualizations of colony formation provide new insights that are motivated by significant implications for both basic research and stem cell-based therapies. |
| first_indexed | 2024-12-21T07:28:25Z |
| format | Article |
| id | doaj.art-6bcfd5e4c7964943a44d4eb0c8523357 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:28:25Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6bcfd5e4c7964943a44d4eb0c85233572022-12-21T19:11:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01144e021345210.1371/journal.pone.0213452Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis.Deena A RennerfeldtJoana S RaminhosSamantha M LeffPristinavae ManningKrystyn J Van VlietBone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the potential for heterogeneity within a single colony forming unit-generated ostensibly from a single mother cell-is less explored but is critical to design of both fundamental studies and cell therapy production. Here we observed BMSC colony formation in real time via time lapsed optical imaging and analysis, to quantify whether and how heterogeneity emerged over multiple cell divisions spanning the duration of a typical colony formation unit assay. These analyses demonstrate that such colonies are neither homogeneous subpopulations of stem cells nor necessarily derived from single originating cells. While the mechanisms for and causes of this intracolony heterogeneity are not understood fully, we further demonstrate that extensive cell-cell contacts do not correlate with senescence, but that media exchange was concurrent with diversification in even the most uniform single-cell-derived colonies. These direct quantitative observations and visualizations of colony formation provide new insights that are motivated by significant implications for both basic research and stem cell-based therapies.https://doi.org/10.1371/journal.pone.0213452 |
| spellingShingle | Deena A Rennerfeldt Joana S Raminhos Samantha M Leff Pristinavae Manning Krystyn J Van Vliet Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. PLoS ONE |
| title | Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. |
| title_full | Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. |
| title_fullStr | Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. |
| title_full_unstemmed | Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. |
| title_short | Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis. |
| title_sort | emergent heterogeneity in putative mesenchymal stem cell colonies single cell time lapsed analysis |
| url | https://doi.org/10.1371/journal.pone.0213452 |
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