Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging
Simple and rapid imaging and analysis of 2D and 3D cell culture compatible with miniaturized arrays of nanoliter droplets are essential for high-throughput screening and personalized medicine applications. In this study, we have developed a simple one-step, cost-effective and sensitive colorimetric...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | SLAS Technology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630321000170 |
| _version_ | 1828420385182318592 |
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| author | Anna A. Popova Markus Reischl Daniel Kazenmaier Haijun Cui Timo Amberger Pavel A. Levkin |
| author_facet | Anna A. Popova Markus Reischl Daniel Kazenmaier Haijun Cui Timo Amberger Pavel A. Levkin |
| author_sort | Anna A. Popova |
| collection | DOAJ |
| description | Simple and rapid imaging and analysis of 2D and 3D cell culture compatible with miniaturized arrays of nanoliter droplets are essential for high-throughput screening and personalized medicine applications. In this study, we have developed a simple one-step, cost-effective and sensitive colorimetric method for the analysis of cell viability in 2D and 3D cell cultures on a nanoliter droplet microarray. The method utilizes a flatbed document scanner that detects a color change in response to cell metabolism in nanoliter droplets with high sensitivity in a single step without the need for expensive specialized equipment. This new nanoliter-based method is faster and more sensitive than equivalent methods using multi-well plate assays. The method detects quantifiable signal from as few as 10 cells and requires only 5 min. This is 2.5 to 10-fold more sensitive and 12 times faster than the same assay in multi-well plates. The method is simple, affordable, fast and sensitive. It can be used for various applications including high-throughput cell-based and biochemical screenings. |
| first_indexed | 2024-12-10T15:11:18Z |
| format | Article |
| id | doaj.art-6bd1d3dcffa44de8b690329655424b3c |
| institution | Directory Open Access Journal |
| issn | 2472-6303 |
| language | English |
| last_indexed | 2024-12-10T15:11:18Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj.art-6bd1d3dcffa44de8b690329655424b3c2022-12-22T01:43:56ZengElsevierSLAS Technology2472-63032022-02-012714453Simple assessment of viability in 2D and 3D cell microarrays using single step digital imagingAnna A. Popova0Markus Reischl1Daniel Kazenmaier2Haijun Cui3Timo Amberger4Pavel A. Levkin5Karlsruhe Institute of Technology, Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; Corresponding authorKarlsruhe Institute of Technology (KIT), Institute for Automation and Applied Informatics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology, Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology, Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology, Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology, Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany; Corresponding authorSimple and rapid imaging and analysis of 2D and 3D cell culture compatible with miniaturized arrays of nanoliter droplets are essential for high-throughput screening and personalized medicine applications. In this study, we have developed a simple one-step, cost-effective and sensitive colorimetric method for the analysis of cell viability in 2D and 3D cell cultures on a nanoliter droplet microarray. The method utilizes a flatbed document scanner that detects a color change in response to cell metabolism in nanoliter droplets with high sensitivity in a single step without the need for expensive specialized equipment. This new nanoliter-based method is faster and more sensitive than equivalent methods using multi-well plate assays. The method detects quantifiable signal from as few as 10 cells and requires only 5 min. This is 2.5 to 10-fold more sensitive and 12 times faster than the same assay in multi-well plates. The method is simple, affordable, fast and sensitive. It can be used for various applications including high-throughput cell-based and biochemical screenings.http://www.sciencedirect.com/science/article/pii/S2472630321000170Droplet microarrayColorimetryHydrophobicityViability assaysDigital imaging |
| spellingShingle | Anna A. Popova Markus Reischl Daniel Kazenmaier Haijun Cui Timo Amberger Pavel A. Levkin Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging SLAS Technology Droplet microarray Colorimetry Hydrophobicity Viability assays Digital imaging |
| title | Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging |
| title_full | Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging |
| title_fullStr | Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging |
| title_full_unstemmed | Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging |
| title_short | Simple assessment of viability in 2D and 3D cell microarrays using single step digital imaging |
| title_sort | simple assessment of viability in 2d and 3d cell microarrays using single step digital imaging |
| topic | Droplet microarray Colorimetry Hydrophobicity Viability assays Digital imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2472630321000170 |
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