Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish
11 active substances used in pesticides were selected. Substances were divided into three groups depending the effect on embryos or fetuses of laboratory animals: 1 – damaging effect on embryos or fetuses (embryotoxic, fetotoxic or teratogenic), 2 – damaging effect on embryos or fetuses, but only at...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750021001712 |
| _version_ | 1819179054529511424 |
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| author | Robert Sornat Joanna Kalka Justyna Faron Marta Napora-Rutkowska Daniel Krakowian Agnieszka Drzewiecka |
| author_facet | Robert Sornat Joanna Kalka Justyna Faron Marta Napora-Rutkowska Daniel Krakowian Agnieszka Drzewiecka |
| author_sort | Robert Sornat |
| collection | DOAJ |
| description | 11 active substances used in pesticides were selected. Substances were divided into three groups depending the effect on embryos or fetuses of laboratory animals: 1 – damaging effect on embryos or fetuses (embryotoxic, fetotoxic or teratogenic), 2 – damaging effect on embryos or fetuses, but only at dose toxic for mother (maternal toxicity), 3 – no damaging effect.Changes for hydra in acute toxicity tests and recovery tests were assessed on an change scale from 0 to 10. The index of the effect on development (TI) for hydras was calculated for every compound. Changes in zebrafish embryos were assessed using a descriptive method. Pearson correlation coefficient showed the correlation between the concentration and the toxic effect in the zebrafish embryos for the substances of the first group.The study showed that substances having a strong damaging effect on fetuses cause changes that are apparent and easy to evaluate both in hydras and zebrafish embryos. A scoring system was introduced to evaluate the changes of hydras and zebrafish embryos. The point system of evaluation of changes allows quick classification of a substance as potentially embryotoxic, fetotoxic or teratogenic. It allows developing a cheap and fast method alternative to prenatal developmental toxicity studies, a screening method that enables substances of great teratogenic potential to be excluded from studies on laboratory animals. |
| first_indexed | 2024-12-22T21:52:21Z |
| format | Article |
| id | doaj.art-6bd2ca9284524c90baba4dc1f32f77f6 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-22T21:52:21Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-6bd2ca9284524c90baba4dc1f32f77f62022-12-21T18:11:20ZengElsevierToxicology Reports2214-75002021-01-01817421753Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafishRobert Sornat0Joanna Kalka1Justyna Faron2Marta Napora-Rutkowska3Daniel Krakowian4Agnieszka Drzewiecka5Łukasiewicz Research Network – Institute of Industrial Organic Chemistry, Branch Pszczyna, Doświadczalna 27, 43-200, Pszczyna, Poland; Silesian University of Technology, The Faculty of Energy and Environmental Engineering, Konarskiego 18, 44-100, Gliwice, Poland; Corresponding author.Silesian University of Technology, The Faculty of Energy and Environmental Engineering, Konarskiego 18, 44-100, Gliwice, PolandŁukasiewicz Research Network – Institute of Industrial Organic Chemistry, Branch Pszczyna, Doświadczalna 27, 43-200, Pszczyna, PolandŁukasiewicz Research Network – Institute of Industrial Organic Chemistry, Branch Pszczyna, Doświadczalna 27, 43-200, Pszczyna, Poland; Veterinary Clinic LUX-VET, Słoneczna 118, 43-384, Jaworze, PolandŁukasiewicz Research Network – Institute of Industrial Organic Chemistry, Branch Pszczyna, Doświadczalna 27, 43-200, Pszczyna, PolandŁukasiewicz Research Network – Institute of Industrial Organic Chemistry, Branch Pszczyna, Doświadczalna 27, 43-200, Pszczyna, Poland11 active substances used in pesticides were selected. Substances were divided into three groups depending the effect on embryos or fetuses of laboratory animals: 1 – damaging effect on embryos or fetuses (embryotoxic, fetotoxic or teratogenic), 2 – damaging effect on embryos or fetuses, but only at dose toxic for mother (maternal toxicity), 3 – no damaging effect.Changes for hydra in acute toxicity tests and recovery tests were assessed on an change scale from 0 to 10. The index of the effect on development (TI) for hydras was calculated for every compound. Changes in zebrafish embryos were assessed using a descriptive method. Pearson correlation coefficient showed the correlation between the concentration and the toxic effect in the zebrafish embryos for the substances of the first group.The study showed that substances having a strong damaging effect on fetuses cause changes that are apparent and easy to evaluate both in hydras and zebrafish embryos. A scoring system was introduced to evaluate the changes of hydras and zebrafish embryos. The point system of evaluation of changes allows quick classification of a substance as potentially embryotoxic, fetotoxic or teratogenic. It allows developing a cheap and fast method alternative to prenatal developmental toxicity studies, a screening method that enables substances of great teratogenic potential to be excluded from studies on laboratory animals.http://www.sciencedirect.com/science/article/pii/S2214750021001712Hydra attenuateZebrafish embryosAlternative methods |
| spellingShingle | Robert Sornat Joanna Kalka Justyna Faron Marta Napora-Rutkowska Daniel Krakowian Agnieszka Drzewiecka Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish Toxicology Reports Hydra attenuate Zebrafish embryos Alternative methods |
| title | Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish |
| title_full | Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish |
| title_fullStr | Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish |
| title_full_unstemmed | Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish |
| title_short | Developing a screening test for toxicity studies of prenatal development with the use of Hydra attenuata and embryos of zebrafish |
| title_sort | developing a screening test for toxicity studies of prenatal development with the use of hydra attenuata and embryos of zebrafish |
| topic | Hydra attenuate Zebrafish embryos Alternative methods |
| url | http://www.sciencedirect.com/science/article/pii/S2214750021001712 |
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