The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation
In the lumen of blood vessels, there are large numbers of erythrocytes, which are approximately 95% of the total blood cells. Although the function of erythrocytes is to transport oxygen in the organism, recent studies have shown that mammalian and teleost erythrocytes are involved in the immune res...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.766970/full |
| _version_ | 1798021578105028608 |
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| author | Youcheng Yang Jiajun Chen Linqing Lu Zizheng Xu Feng Li Minxuan Yang Jun Li Jun Li Li Lin Zhendong Qin |
| author_facet | Youcheng Yang Jiajun Chen Linqing Lu Zizheng Xu Feng Li Minxuan Yang Jun Li Jun Li Li Lin Zhendong Qin |
| author_sort | Youcheng Yang |
| collection | DOAJ |
| description | In the lumen of blood vessels, there are large numbers of erythrocytes, which are approximately 95% of the total blood cells. Although the function of erythrocytes is to transport oxygen in the organism, recent studies have shown that mammalian and teleost erythrocytes are involved in the immune response against bacterial infections. However, the immune mechanisms used by avian erythrocytes are not yet clear. Here, we demonstrated that erythrocytes from goose have the ability to phagocytose as well as conduct antimicrobial activity. Firstly, we revealed the phagocytosis or adhesion activity of goose erythrocytes for latex beads 0.1-1.0 μm in diameter by fluorescence microscopy, and scanning and transmission electron microscopy. The low cytometry results also proved that goose erythrocytes had a wide range of phagocytic or adhesion activity for different bacteria. Followed, the low cytometry analysis data further explored that the goose erythrocytes contain the ability to produce reactive oxygen species (ROS) and inducible nitric oxide synthase (iNOS) in response to bacterial stimulation, and also up-regulated the expression of NOX family includes NOX1 and NOX5. Finally, we also found that goose erythrocytes showed a powerful antibacterial activity against all the three bacteria, meanwhile the stimulation of three kinds of bacteria up-regulated the expression of inflammatory factors, and increased the production of antioxidant enzymes to protect the cells from oxidative damage. Herein, our results demonstrate that goose Erythrocytes possess a certain phagocytic capacity and antioxidant system, and that the antimicrobial activity of erythrocytes can occurred through the production of unique respiratory burst against foreign pathogenic bacteria, which provides new clues to the interaction between bacteria and avian erythrocytes. |
| first_indexed | 2024-04-11T17:15:50Z |
| format | Article |
| id | doaj.art-6bd5bbcd0a89473aadfc7fe4582202f7 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T17:15:50Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6bd5bbcd0a89473aadfc7fe4582202f72022-12-22T04:12:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-01-011210.3389/fimmu.2021.766970766970The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst GenerationYoucheng Yang0Jiajun Chen1Linqing Lu2Zizheng Xu3Feng Li4Minxuan Yang5Jun Li6Jun Li7Li Lin8Zhendong Qin9Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaSchool of Science and Medicine, Lake Superior State University, Sault Ste. Marie, MI, United StatesGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaIn the lumen of blood vessels, there are large numbers of erythrocytes, which are approximately 95% of the total blood cells. Although the function of erythrocytes is to transport oxygen in the organism, recent studies have shown that mammalian and teleost erythrocytes are involved in the immune response against bacterial infections. However, the immune mechanisms used by avian erythrocytes are not yet clear. Here, we demonstrated that erythrocytes from goose have the ability to phagocytose as well as conduct antimicrobial activity. Firstly, we revealed the phagocytosis or adhesion activity of goose erythrocytes for latex beads 0.1-1.0 μm in diameter by fluorescence microscopy, and scanning and transmission electron microscopy. The low cytometry results also proved that goose erythrocytes had a wide range of phagocytic or adhesion activity for different bacteria. Followed, the low cytometry analysis data further explored that the goose erythrocytes contain the ability to produce reactive oxygen species (ROS) and inducible nitric oxide synthase (iNOS) in response to bacterial stimulation, and also up-regulated the expression of NOX family includes NOX1 and NOX5. Finally, we also found that goose erythrocytes showed a powerful antibacterial activity against all the three bacteria, meanwhile the stimulation of three kinds of bacteria up-regulated the expression of inflammatory factors, and increased the production of antioxidant enzymes to protect the cells from oxidative damage. Herein, our results demonstrate that goose Erythrocytes possess a certain phagocytic capacity and antioxidant system, and that the antimicrobial activity of erythrocytes can occurred through the production of unique respiratory burst against foreign pathogenic bacteria, which provides new clues to the interaction between bacteria and avian erythrocytes.https://www.frontiersin.org/articles/10.3389/fimmu.2021.766970/fullred blood cells (RBCs)Anser domesticusrespiratory burstphagocytosisantibacterial activity |
| spellingShingle | Youcheng Yang Jiajun Chen Linqing Lu Zizheng Xu Feng Li Minxuan Yang Jun Li Jun Li Li Lin Zhendong Qin The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation Frontiers in Immunology red blood cells (RBCs) Anser domesticus respiratory burst phagocytosis antibacterial activity |
| title | The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation |
| title_full | The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation |
| title_fullStr | The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation |
| title_full_unstemmed | The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation |
| title_short | The Antibacterial Activity of Erythrocytes From Goose (Anser domesticus) Can Be Associated With Phagocytosis and Respiratory Burst Generation |
| title_sort | antibacterial activity of erythrocytes from goose anser domesticus can be associated with phagocytosis and respiratory burst generation |
| topic | red blood cells (RBCs) Anser domesticus respiratory burst phagocytosis antibacterial activity |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.766970/full |
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