T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM). However, MSDM is sometimes difficult. Here we demonstrated that...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-07-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1170630 |
| _version_ | 1818312173826342912 |
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| author | Wei Guo Shuhui Sun Binghua Xie Xiao-Jing Zhu Zhong-Min Dai |
| author_facet | Wei Guo Shuhui Sun Binghua Xie Xiao-Jing Zhu Zhong-Min Dai |
| author_sort | Wei Guo |
| collection | DOAJ |
| description | Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM). However, MSDM is sometimes difficult. Here we demonstrated that T4 DNA polymerase greatly enhanced the efficiency of MSDM. Moreover, we have also showed that it is efficient to clone multiple specific mutation-containing sequences simultaneously. |
| first_indexed | 2024-12-13T08:13:39Z |
| format | Article |
| id | doaj.art-6bd9f46216594cabaea1186f631c174b |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-13T08:13:39Z |
| publishDate | 2016-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-6bd9f46216594cabaea1186f631c174b2022-12-21T23:54:10ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-07-0130472172510.1080/13102818.2016.11706301170630T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesisWei Guo0Shuhui Sun1Binghua Xie2Xiao-Jing Zhu3Zhong-Min Dai4Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal UniversityInstitute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal UniversityInstitute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal UniversityInstitute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal UniversityInstitute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal UniversitySite-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM). However, MSDM is sometimes difficult. Here we demonstrated that T4 DNA polymerase greatly enhanced the efficiency of MSDM. Moreover, we have also showed that it is efficient to clone multiple specific mutation-containing sequences simultaneously.http://dx.doi.org/10.1080/13102818.2016.1170630Multiple site-directed mutagenesiscomplementary annealing mediated by exonucleaseT4 DNA polymeraseDNA assembly |
| spellingShingle | Wei Guo Shuhui Sun Binghua Xie Xiao-Jing Zhu Zhong-Min Dai T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis Biotechnology & Biotechnological Equipment Multiple site-directed mutagenesis complementary annealing mediated by exonuclease T4 DNA polymerase DNA assembly |
| title | T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis |
| title_full | T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis |
| title_fullStr | T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis |
| title_full_unstemmed | T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis |
| title_short | T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis |
| title_sort | t4 dna polymerase improves the efficiency of multiple site directed mutagenesis |
| topic | Multiple site-directed mutagenesis complementary annealing mediated by exonuclease T4 DNA polymerase DNA assembly |
| url | http://dx.doi.org/10.1080/13102818.2016.1170630 |
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