Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome
IntroductionThe low pregnancy rate by artificial insemination in sheep represents a fundamental challenge for breeding programs. In this species, oestrus synchronization is carried out by manipulating hormonal regimens through the insertion of progestogen intravaginal devices. This reproductive stra...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1063807/full |
| _version_ | 1797862182799540224 |
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| author | Edgar L. Reinoso-Peláez Edgar L. Reinoso-Peláez María Saura Óscar González-Recio Carmen González Almudena Fernández Ramón Peiro-Pastor Adrián López-García Alejandro Saborío-Montero Jorge H. Calvo Manuel Ramón Malena Serrano |
| author_facet | Edgar L. Reinoso-Peláez Edgar L. Reinoso-Peláez María Saura Óscar González-Recio Carmen González Almudena Fernández Ramón Peiro-Pastor Adrián López-García Alejandro Saborío-Montero Jorge H. Calvo Manuel Ramón Malena Serrano |
| author_sort | Edgar L. Reinoso-Peláez |
| collection | DOAJ |
| description | IntroductionThe low pregnancy rate by artificial insemination in sheep represents a fundamental challenge for breeding programs. In this species, oestrus synchronization is carried out by manipulating hormonal regimens through the insertion of progestogen intravaginal devices. This reproductive strategy may alter the vaginal microbiota affecting the artificial insemination outcome.MethodsIn this study, we analyzed the vaginal microbiome of 94 vaginal swabs collected from 47 ewes with alternative treatments applied to the progesterone-releasing intravaginal devices (probiotic, maltodextrin, antibiotic and control), in two sample periods (before placing and after removing the devices). To our knowledge, this is the first study using nanopore-based metagenome sequencing for vaginal microbiome characterization in livestock.ResultsOur results revealed a significant lower abundance of the genera Oenococcus (Firmicutes) and Neisseria (Proteobacteria) in pregnant compared to non-pregnant ewes. We also detected a significant lower abundance of Campylobacter in the group of samples treated with the probiotic.DiscussionAlthough the use of probiotics represents a promising practice to improve insemination results, the election of the suitable species and concentration requires further investigation. In addition, the use of progestogen in the synchronization devices seemed to increase the alpha-diversity and decrease the abundance of harmful microorganisms belonging to Gammaproteobacteria and Fusobacteriia classes, suggesting a beneficial effect of their use. |
| first_indexed | 2024-04-09T22:16:10Z |
| format | Article |
| id | doaj.art-6bdd93f1539c4695a32a30938f172e5a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T22:16:10Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6bdd93f1539c4695a32a30938f172e5a2023-03-23T04:33:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.10638071063807Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcomeEdgar L. Reinoso-Peláez0Edgar L. Reinoso-Peláez1María Saura2Óscar González-Recio3Carmen González4Almudena Fernández5Ramón Peiro-Pastor6Adrián López-García7Alejandro Saborío-Montero8Jorge H. Calvo9Manuel Ramón10Malena Serrano11Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Producción Agraria, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainDepartamento de Ciencia Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA-ARAID-IA2), Zaragoza, SpainDepartamento de Investigación en Reproducción y Mejora Genética Animal, Centro Regional de Selección y Reproducción Animal de Castilla La Mancha (CERSYRA-IRIAF), Valdepeñas, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, SpainIntroductionThe low pregnancy rate by artificial insemination in sheep represents a fundamental challenge for breeding programs. In this species, oestrus synchronization is carried out by manipulating hormonal regimens through the insertion of progestogen intravaginal devices. This reproductive strategy may alter the vaginal microbiota affecting the artificial insemination outcome.MethodsIn this study, we analyzed the vaginal microbiome of 94 vaginal swabs collected from 47 ewes with alternative treatments applied to the progesterone-releasing intravaginal devices (probiotic, maltodextrin, antibiotic and control), in two sample periods (before placing and after removing the devices). To our knowledge, this is the first study using nanopore-based metagenome sequencing for vaginal microbiome characterization in livestock.ResultsOur results revealed a significant lower abundance of the genera Oenococcus (Firmicutes) and Neisseria (Proteobacteria) in pregnant compared to non-pregnant ewes. We also detected a significant lower abundance of Campylobacter in the group of samples treated with the probiotic.DiscussionAlthough the use of probiotics represents a promising practice to improve insemination results, the election of the suitable species and concentration requires further investigation. In addition, the use of progestogen in the synchronization devices seemed to increase the alpha-diversity and decrease the abundance of harmful microorganisms belonging to Gammaproteobacteria and Fusobacteriia classes, suggesting a beneficial effect of their use.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1063807/fullartificial inseminationfertilitynanoporeovinereproductionvaginal microbiota |
| spellingShingle | Edgar L. Reinoso-Peláez Edgar L. Reinoso-Peláez María Saura Óscar González-Recio Carmen González Almudena Fernández Ramón Peiro-Pastor Adrián López-García Alejandro Saborío-Montero Jorge H. Calvo Manuel Ramón Malena Serrano Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome Frontiers in Microbiology artificial insemination fertility nanopore ovine reproduction vaginal microbiota |
| title | Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| title_full | Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| title_fullStr | Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| title_full_unstemmed | Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| title_short | Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| title_sort | impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome |
| topic | artificial insemination fertility nanopore ovine reproduction vaginal microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1063807/full |
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