A simple method for the separation of triacylglycerols from fatty acids released in lipase assays

A simple procedure for the partition of triacylglycerols from albumin-bound fatty acids is described. This procedure is based on the ability of fumed silicon dioxide to remove emulsified triacylglycerols from aqueous media. The method was developed for the assay of lipoprotein lipase activity but it...

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Main Authors: J Borensztajn, M N Reddy, A R Gladstone
Format: Article
Language:English
Published: Elsevier 1988-11-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520384327
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author J Borensztajn
M N Reddy
A R Gladstone
author_facet J Borensztajn
M N Reddy
A R Gladstone
author_sort J Borensztajn
collection DOAJ
description A simple procedure for the partition of triacylglycerols from albumin-bound fatty acids is described. This procedure is based on the ability of fumed silicon dioxide to remove emulsified triacylglycerols from aqueous media. The method was developed for the assay of lipoprotein lipase activity but it may be used for the assay of other lipases.
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spelling doaj.art-6be0a5052f3b42b5b158764110fff1e72022-12-21T20:26:31ZengElsevierJournal of Lipid Research0022-22751988-11-01291115491552A simple method for the separation of triacylglycerols from fatty acids released in lipase assaysJ Borensztajn0M N Reddy1A R Gladstone2Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.A simple procedure for the partition of triacylglycerols from albumin-bound fatty acids is described. This procedure is based on the ability of fumed silicon dioxide to remove emulsified triacylglycerols from aqueous media. The method was developed for the assay of lipoprotein lipase activity but it may be used for the assay of other lipases.http://www.sciencedirect.com/science/article/pii/S0022227520384327
spellingShingle J Borensztajn
M N Reddy
A R Gladstone
A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
Journal of Lipid Research
title A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
title_full A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
title_fullStr A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
title_full_unstemmed A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
title_short A simple method for the separation of triacylglycerols from fatty acids released in lipase assays
title_sort simple method for the separation of triacylglycerols from fatty acids released in lipase assays
url http://www.sciencedirect.com/science/article/pii/S0022227520384327
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