Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury
Influenza remains one of the major epidemic diseases worldwide. Acute lung injury mainly caused by excessive pro-inflammatory host immune responses leads to high mortality rates in severe influenza patients. Galectin-1, an animal lectin ubiquitously expressed in mammalian tissues, is reported to pla...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01293/full |
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| author | Jiaqi Bao Jiaqi Bao Jiaqi Bao Xiaochen Wang Xiaochen Wang Xiaochen Wang Sijia Liu Sijia Liu Sijia Liu Qianda Zou Qianda Zou Qianda Zou Shufa Zheng Shufa Zheng Shufa Zheng Shufa Zheng Fei Yu Fei Yu Fei Yu Yu Chen Yu Chen Yu Chen Yu Chen |
| author_facet | Jiaqi Bao Jiaqi Bao Jiaqi Bao Xiaochen Wang Xiaochen Wang Xiaochen Wang Sijia Liu Sijia Liu Sijia Liu Qianda Zou Qianda Zou Qianda Zou Shufa Zheng Shufa Zheng Shufa Zheng Shufa Zheng Fei Yu Fei Yu Fei Yu Yu Chen Yu Chen Yu Chen Yu Chen |
| author_sort | Jiaqi Bao |
| collection | DOAJ |
| description | Influenza remains one of the major epidemic diseases worldwide. Acute lung injury mainly caused by excessive pro-inflammatory host immune responses leads to high mortality rates in severe influenza patients. Galectin-1, an animal lectin ubiquitously expressed in mammalian tissues, is reported to play important roles in viral diseases. Here, we established murine and A549 cell models to explore the potential roles of galectin-1 treatment in H1N1pdm09-induced acute lung injury. We found that galectin-1 protein level was elevated in A549 cell culture supernatants and mouse BALF after H1N1pdm09 challenge. In vivo experiments showed recombinant galectin-1 treatment reduced wet/dry weight ratio, inflammatory cell infiltration in mouse lungs and mediated the expression of cytokines and chemokines including IL-1β, IL-6, IL-10, IL-12(p40), IL-12(p70), G-CSF, MCP-1, MIP-1α and RANTES in serum and BALF of infected mice. Reduced apoptosis and viral titers in mouse lungs were also found after galectin-1 treatment. As expected, galectin-1 treated mice performed reduced body weight loss and enhanced survival rate against H1N1pdm09 challenge. In addition, in vitro experiments showed that viral titers decreased in a dose-dependent manner and cell apoptosis in A549 cells reduced after recombinant galectin-1 treatment. Taken together, our findings indicate a potentially positive effect of Gal-1 treatment on ameliorating the progress of H1N1pdm09-induced acute lung injury and recombinant galectin-1 might serve as a new agent in treating influenza. |
| first_indexed | 2024-12-22T21:28:20Z |
| format | Article |
| id | doaj.art-6be1ce228ec74c368e71337d3aaf206e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T21:28:20Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-6be1ce228ec74c368e71337d3aaf206e2022-12-21T18:11:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01293544138Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung InjuryJiaqi Bao0Jiaqi Bao1Jiaqi Bao2Xiaochen Wang3Xiaochen Wang4Xiaochen Wang5Sijia Liu6Sijia Liu7Sijia Liu8Qianda Zou9Qianda Zou10Qianda Zou11Shufa Zheng12Shufa Zheng13Shufa Zheng14Shufa Zheng15Fei Yu16Fei Yu17Fei Yu18Yu Chen19Yu Chen20Yu Chen21Yu Chen22Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, ChinaInstitute of Laboratory Medicine, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaInfluenza remains one of the major epidemic diseases worldwide. Acute lung injury mainly caused by excessive pro-inflammatory host immune responses leads to high mortality rates in severe influenza patients. Galectin-1, an animal lectin ubiquitously expressed in mammalian tissues, is reported to play important roles in viral diseases. Here, we established murine and A549 cell models to explore the potential roles of galectin-1 treatment in H1N1pdm09-induced acute lung injury. We found that galectin-1 protein level was elevated in A549 cell culture supernatants and mouse BALF after H1N1pdm09 challenge. In vivo experiments showed recombinant galectin-1 treatment reduced wet/dry weight ratio, inflammatory cell infiltration in mouse lungs and mediated the expression of cytokines and chemokines including IL-1β, IL-6, IL-10, IL-12(p40), IL-12(p70), G-CSF, MCP-1, MIP-1α and RANTES in serum and BALF of infected mice. Reduced apoptosis and viral titers in mouse lungs were also found after galectin-1 treatment. As expected, galectin-1 treated mice performed reduced body weight loss and enhanced survival rate against H1N1pdm09 challenge. In addition, in vitro experiments showed that viral titers decreased in a dose-dependent manner and cell apoptosis in A549 cells reduced after recombinant galectin-1 treatment. Taken together, our findings indicate a potentially positive effect of Gal-1 treatment on ameliorating the progress of H1N1pdm09-induced acute lung injury and recombinant galectin-1 might serve as a new agent in treating influenza.https://www.frontiersin.org/article/10.3389/fmicb.2020.01293/fullinfluenza virusH1N1pdm09galectin-1acute lung injuryinflammationreplication |
| spellingShingle | Jiaqi Bao Jiaqi Bao Jiaqi Bao Xiaochen Wang Xiaochen Wang Xiaochen Wang Sijia Liu Sijia Liu Sijia Liu Qianda Zou Qianda Zou Qianda Zou Shufa Zheng Shufa Zheng Shufa Zheng Shufa Zheng Fei Yu Fei Yu Fei Yu Yu Chen Yu Chen Yu Chen Yu Chen Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury Frontiers in Microbiology influenza virus H1N1pdm09 galectin-1 acute lung injury inflammation replication |
| title | Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury |
| title_full | Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury |
| title_fullStr | Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury |
| title_full_unstemmed | Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury |
| title_short | Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury |
| title_sort | galectin 1 ameliorates influenza a h1n1pdm09 virus induced acute lung injury |
| topic | influenza virus H1N1pdm09 galectin-1 acute lung injury inflammation replication |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01293/full |
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