Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study
Objectives: Since time immemorial, plants have continued to play a predominant role in the maintenance of human health as sources of medicinal compounds. Several effective antifungal agents are available for oral Candida infections; the failure is not uncommon because isolates of Candida albicans ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | The Journal of Indian Prosthodontic Society |
| Subjects: | |
| Online Access: | http://www.j-ips.org/article.asp?issn=0972-4052;year=2017;volume=17;issue=3;spage=267;epage=272;aulast=Iyer |
| _version_ | 1818994432941228032 |
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| author | Meenakshi Iyer Anil Kumar Gujjari Vishakante Gowda Shridhar Angadi |
| author_facet | Meenakshi Iyer Anil Kumar Gujjari Vishakante Gowda Shridhar Angadi |
| author_sort | Meenakshi Iyer |
| collection | DOAJ |
| description | Objectives: Since time immemorial, plants have continued to play a predominant role in the maintenance of human health as sources of medicinal compounds. Several effective antifungal agents are available for oral Candida infections; the failure is not uncommon because isolates of Candida albicans may exhibit resistance to the drug during therapy. The present study aimed to identify an alternative, inexpensive, simple, and effective method of preventing and controlling the candidal infection.
Methodology: All the procured and authenticated nutmeg seeds were dried in shade and cleaned by hand sorting. The crushed seeds were passed through mesh no. 40 individually. About 50 g of powdered nutmeg seeds was loaded in the supercritical fluid extractor unit using supercritical CO2 as extracting solvent in accordance with the methods of Nguyen et al. Supercritical fluid (SFE) extraction was done using CO2 gas without any cosolvents.
Results: The nutmeg extract displayed antifungal activity with the effective zone of inhibition ranging from 18.0 to 12.0 mm when compared with nystatin as positive control.
Conclusion: This paper described the in vitro antibacterial activity, and phytochemical analysis of SFE extract of nutmeg (Myristica fragrans) evaluated against C. albicans (American Type Culture Collection 10231) through agar well diffusion method. SFE of nutmeg seeds can be used as an adjunct to conventional therapy for oral candidiasis. |
| first_indexed | 2024-12-20T20:57:52Z |
| format | Article |
| id | doaj.art-6be47d3c5f01457b9fcfc4b62dc0a7c1 |
| institution | Directory Open Access Journal |
| issn | 0972-4052 1998-4057 |
| language | English |
| last_indexed | 2024-12-20T20:57:52Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Journal of Indian Prosthodontic Society |
| spelling | doaj.art-6be47d3c5f01457b9fcfc4b62dc0a7c12022-12-21T19:26:47ZengWolters Kluwer Medknow PublicationsThe Journal of Indian Prosthodontic Society0972-40521998-40572017-01-0117326727210.4103/jips.jips_10_17Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening studyMeenakshi IyerAnil Kumar GujjariVishakante GowdaShridhar AngadiObjectives: Since time immemorial, plants have continued to play a predominant role in the maintenance of human health as sources of medicinal compounds. Several effective antifungal agents are available for oral Candida infections; the failure is not uncommon because isolates of Candida albicans may exhibit resistance to the drug during therapy. The present study aimed to identify an alternative, inexpensive, simple, and effective method of preventing and controlling the candidal infection. Methodology: All the procured and authenticated nutmeg seeds were dried in shade and cleaned by hand sorting. The crushed seeds were passed through mesh no. 40 individually. About 50 g of powdered nutmeg seeds was loaded in the supercritical fluid extractor unit using supercritical CO2 as extracting solvent in accordance with the methods of Nguyen et al. Supercritical fluid (SFE) extraction was done using CO2 gas without any cosolvents. Results: The nutmeg extract displayed antifungal activity with the effective zone of inhibition ranging from 18.0 to 12.0 mm when compared with nystatin as positive control. Conclusion: This paper described the in vitro antibacterial activity, and phytochemical analysis of SFE extract of nutmeg (Myristica fragrans) evaluated against C. albicans (American Type Culture Collection 10231) through agar well diffusion method. SFE of nutmeg seeds can be used as an adjunct to conventional therapy for oral candidiasis.http://www.j-ips.org/article.asp?issn=0972-4052;year=2017;volume=17;issue=3;spage=267;epage=272;aulast=IyerAlternative medicineantimicrobial activityCandida albicanscomplementary therapiesphytotherapyplant extractssupercritical fluid extraction |
| spellingShingle | Meenakshi Iyer Anil Kumar Gujjari Vishakante Gowda Shridhar Angadi Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study The Journal of Indian Prosthodontic Society Alternative medicine antimicrobial activity Candida albicans complementary therapies phytotherapy plant extracts supercritical fluid extraction |
| title | Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study |
| title_full | Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study |
| title_fullStr | Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study |
| title_full_unstemmed | Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study |
| title_short | Antifungal response of or-al-associated candidal reference strains (American Type Culture Collection) by supercritical fluid extract of nutmeg seeds for geriatric denture wearers: An in vitro screening study |
| title_sort | antifungal response of or al associated candidal reference strains american type culture collection by supercritical fluid extract of nutmeg seeds for geriatric denture wearers an in vitro screening study |
| topic | Alternative medicine antimicrobial activity Candida albicans complementary therapies phytotherapy plant extracts supercritical fluid extraction |
| url | http://www.j-ips.org/article.asp?issn=0972-4052;year=2017;volume=17;issue=3;spage=267;epage=272;aulast=Iyer |
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