Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection
Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoni...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
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Hamadan University of Medical Sciences
1999-09-01
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| Series: | پزشکی بالینی ابن سینا |
| Subjects: | |
| Online Access: | http://sjh.umsha.ac.ir/article-1-983-en.html |
| _version_ | 1828355084508987392 |
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| author | Masoud Hajia Cheristopher Storey |
| author_facet | Masoud Hajia Cheristopher Storey |
| author_sort | Masoud Hajia |
| collection | DOAJ |
| description | Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications.
To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this organism using DNA-recombinant technology. After optimizing the test, technique was evaluated with 106 throat swabs. Obtained results showed 4 amplified specimens by PCR was positive using agarose gel electrophoresis, but thirty was positive by PCR-hybridization. |
| first_indexed | 2024-04-14T02:35:36Z |
| format | Article |
| id | doaj.art-6be832a8ec574e14af8e1426dfa26ec6 |
| institution | Directory Open Access Journal |
| issn | 2588-722X 2588-7238 |
| language | fas |
| last_indexed | 2024-04-14T02:35:36Z |
| publishDate | 1999-09-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | پزشکی بالینی ابن سینا |
| spelling | doaj.art-6be832a8ec574e14af8e1426dfa26ec62022-12-22T02:17:27ZfasHamadan University of Medical Sciencesپزشکی بالینی ابن سینا2588-722X2588-72381999-09-016200Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae InfectionMasoud Hajia0Cheristopher Storey1 Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this organism using DNA-recombinant technology. After optimizing the test, technique was evaluated with 106 throat swabs. Obtained results showed 4 amplified specimens by PCR was positive using agarose gel electrophoresis, but thirty was positive by PCR-hybridization.http://sjh.umsha.ac.ir/article-1-983-en.htmlhybridization / mycoplasma infection – diagnosis / polymerase chain reaction. |
| spellingShingle | Masoud Hajia Cheristopher Storey Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection پزشکی بالینی ابن سینا hybridization / mycoplasma infection – diagnosis / polymerase chain reaction. |
| title | Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection |
| title_full | Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection |
| title_fullStr | Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection |
| title_full_unstemmed | Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection |
| title_short | Application of PCR-Hybridization in Diagnosis of Mycoplasma Pneumoniae Infection |
| title_sort | application of pcr hybridization in diagnosis of mycoplasma pneumoniae infection |
| topic | hybridization / mycoplasma infection – diagnosis / polymerase chain reaction. |
| url | http://sjh.umsha.ac.ir/article-1-983-en.html |
| work_keys_str_mv | AT masoudhajia applicationofpcrhybridizationindiagnosisofmycoplasmapneumoniaeinfection AT cheristopherstorey applicationofpcrhybridizationindiagnosisofmycoplasmapneumoniaeinfection |