The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea
Background: Enteroaggregative Escherichia coli (EAEC) has recently been identified as the cause of epidemic and endemic chronic watery diarrhea worldwide, especially in developing countries. The aim of this study was to determine the frequency of this strain and its important virulence genes in diar...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2021-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/13756 |
| _version_ | 1827885573095817216 |
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| author | Tahereh Alipour Farkhondeh Poursina |
| author_facet | Tahereh Alipour Farkhondeh Poursina |
| author_sort | Tahereh Alipour |
| collection | DOAJ |
| description | Background: Enteroaggregative Escherichia coli (EAEC) has recently been identified as the cause of epidemic and endemic chronic watery diarrhea worldwide, especially in developing countries. The aim of this study was to determine the frequency of this strain and its important virulence genes in diarrhea samples.
Methods: This study was performed on 80 Escherichia coli isolates from children with diarrhea referred to Imam Hossein hospital, Isfahan, Iran. The bacteria were confirmed using biochemical tests and multiplex polymerase chain reaction (multiplex PCR) was performed to detect aggR, aap, aatA genes in EAEC strains.
Findings: The frequency of EAEC was 21 (26.6%). According to multiplex PCR results, the aggR, aap, and aatA genes were detected in 21 (100%), 7 (33%), and 3 (14.3%) of strains, respectively. Moreover, 7 strains (33.3%) harbored two aggR and aap genes, 3 (14.3%) carried the aatA and aggR genes, and aatA and aap genes were presented in 2 strains (9.5%). None of the strains had 3 genes simultaneously.
Conclusion: This study provides the first report of a relatively high frequency of typical EAEC strains in children under 5 years of age with symptoms of diarrheal disease in Isfahan. Moreover, we found that the EAEC strains not only exhibited high frequency, but also showed a high frequency of carrying virulence genes especially aggR that indicated the importance of identifying this strain in patients showing the symptoms of acute and chronic diarrhea, especially in our country, as the diagnosis of this strain is not done routinely. |
| first_indexed | 2024-03-12T19:48:11Z |
| format | Article |
| id | doaj.art-6be979d0f46f49f3981c7c3520fec220 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T19:48:11Z |
| publishDate | 2021-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-6be979d0f46f49f3981c7c3520fec2202023-08-02T03:22:10ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2021-03-0139609131910.22122/jims.v39i609.137563769The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with DiarrheaTahereh Alipour0Farkhondeh Poursina1MSc Student, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Enteroaggregative Escherichia coli (EAEC) has recently been identified as the cause of epidemic and endemic chronic watery diarrhea worldwide, especially in developing countries. The aim of this study was to determine the frequency of this strain and its important virulence genes in diarrhea samples. Methods: This study was performed on 80 Escherichia coli isolates from children with diarrhea referred to Imam Hossein hospital, Isfahan, Iran. The bacteria were confirmed using biochemical tests and multiplex polymerase chain reaction (multiplex PCR) was performed to detect aggR, aap, aatA genes in EAEC strains. Findings: The frequency of EAEC was 21 (26.6%). According to multiplex PCR results, the aggR, aap, and aatA genes were detected in 21 (100%), 7 (33%), and 3 (14.3%) of strains, respectively. Moreover, 7 strains (33.3%) harbored two aggR and aap genes, 3 (14.3%) carried the aatA and aggR genes, and aatA and aap genes were presented in 2 strains (9.5%). None of the strains had 3 genes simultaneously. Conclusion: This study provides the first report of a relatively high frequency of typical EAEC strains in children under 5 years of age with symptoms of diarrheal disease in Isfahan. Moreover, we found that the EAEC strains not only exhibited high frequency, but also showed a high frequency of carrying virulence genes especially aggR that indicated the importance of identifying this strain in patients showing the symptoms of acute and chronic diarrhea, especially in our country, as the diagnosis of this strain is not done routinely.http://jims.mui.ac.ir/index.php/jims/article/view/13756childrendiarrheaenteroaggregative escherichia coligenesmultiplex polymerase chain reaction |
| spellingShingle | Tahereh Alipour Farkhondeh Poursina The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea مجله دانشکده پزشکی اصفهان children diarrhea enteroaggregative escherichia coli genes multiplex polymerase chain reaction |
| title | The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea |
| title_full | The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea |
| title_fullStr | The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea |
| title_full_unstemmed | The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea |
| title_short | The Frequency of Enteroaggregative Escherichia Coli and Important Virulence Genes Isolated from Children with Diarrhea |
| title_sort | frequency of enteroaggregative escherichia coli and important virulence genes isolated from children with diarrhea |
| topic | children diarrhea enteroaggregative escherichia coli genes multiplex polymerase chain reaction |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/13756 |
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