Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer
<p>Abstract</p> <p>Background</p> <p>Immunohistochemistry (IHC) with mutation-specific antibodies may be an ancillary method of detecting EGFR mutations in lung cancer patients.</p> <p>Methods</p> <p>EGFR mutation status was analyzed by DNA assay...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-12-01
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| Series: | Journal of Translational Medicine |
| Online Access: | http://www.translational-medicine.com/content/8/1/135 |
| _version_ | 1830281431719346176 |
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| author | Viteri Santiago Moran Teresa Isla Dolores Jimenez Ulpiano Gonzalez-Larriba Jose Sanchez José Bertran-Alamillo Jordi Mayo Clara de Aguirre Itziar Queralt Cristina Molina Miguel Simonetti Sara Camps Carlos Garcia-Campelo Rosario Massuti Bartomeu Benlloch Susana y Cajal Santiago Taron Miquel Rosell Rafael |
| author_facet | Viteri Santiago Moran Teresa Isla Dolores Jimenez Ulpiano Gonzalez-Larriba Jose Sanchez José Bertran-Alamillo Jordi Mayo Clara de Aguirre Itziar Queralt Cristina Molina Miguel Simonetti Sara Camps Carlos Garcia-Campelo Rosario Massuti Bartomeu Benlloch Susana y Cajal Santiago Taron Miquel Rosell Rafael |
| author_sort | Viteri Santiago |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Immunohistochemistry (IHC) with mutation-specific antibodies may be an ancillary method of detecting EGFR mutations in lung cancer patients.</p> <p>Methods</p> <p>EGFR mutation status was analyzed by DNA assays, and compared with IHC results in five non-small-cell lung cancer (NSCLC) cell lines and tumor samples from 78 stage IV NSCLC patients.</p> <p>Results</p> <p>IHC correctly identified del 19 in the H1650 and PC9 cell lines, L858R in H1975, and wild-type EGFR in H460 and A549, as well as wild-type EGFR in tumor samples from 22 patients. IHC with the mAb against EGFR with del 19 was highly positive for the protein in all 17 patients with a 15-bp (ELREA) deletion in exon 19, whereas in patients with other deletions, IHC was weakly positive in 3 cases and negative in 9 cases. IHC with the mAb against the L858R mutation showed high positivity for the protein in 25/27 (93%) patients with exon 21 EGFR mutations (all with L858R) but did not identify the L861Q mutation in the remaining two patients.</p> <p>Conclusions</p> <p>IHC with mutation-specific mAbs against EGFR is a promising method for detecting EGFR mutations in NSCLC patients. However these mAbs should be validated with additional studies to clarify their possible role in routine clinical practice for screening EGFR mutations in NSCLC patients.</p> |
| first_indexed | 2024-12-19T02:18:55Z |
| format | Article |
| id | doaj.art-6be97de56801425e9677bdcfa049751b |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-19T02:18:55Z |
| publishDate | 2010-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-6be97de56801425e9677bdcfa049751b2022-12-21T20:40:19ZengBMCJournal of Translational Medicine1479-58762010-12-018113510.1186/1479-5876-8-135Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancerViteri SantiagoMoran TeresaIsla DoloresJimenez UlpianoGonzalez-Larriba JoseSanchez JoséBertran-Alamillo JordiMayo Clarade Aguirre ItziarQueralt CristinaMolina MiguelSimonetti SaraCamps CarlosGarcia-Campelo RosarioMassuti BartomeuBenlloch Susanay Cajal SantiagoTaron MiquelRosell Rafael<p>Abstract</p> <p>Background</p> <p>Immunohistochemistry (IHC) with mutation-specific antibodies may be an ancillary method of detecting EGFR mutations in lung cancer patients.</p> <p>Methods</p> <p>EGFR mutation status was analyzed by DNA assays, and compared with IHC results in five non-small-cell lung cancer (NSCLC) cell lines and tumor samples from 78 stage IV NSCLC patients.</p> <p>Results</p> <p>IHC correctly identified del 19 in the H1650 and PC9 cell lines, L858R in H1975, and wild-type EGFR in H460 and A549, as well as wild-type EGFR in tumor samples from 22 patients. IHC with the mAb against EGFR with del 19 was highly positive for the protein in all 17 patients with a 15-bp (ELREA) deletion in exon 19, whereas in patients with other deletions, IHC was weakly positive in 3 cases and negative in 9 cases. IHC with the mAb against the L858R mutation showed high positivity for the protein in 25/27 (93%) patients with exon 21 EGFR mutations (all with L858R) but did not identify the L861Q mutation in the remaining two patients.</p> <p>Conclusions</p> <p>IHC with mutation-specific mAbs against EGFR is a promising method for detecting EGFR mutations in NSCLC patients. However these mAbs should be validated with additional studies to clarify their possible role in routine clinical practice for screening EGFR mutations in NSCLC patients.</p>http://www.translational-medicine.com/content/8/1/135 |
| spellingShingle | Viteri Santiago Moran Teresa Isla Dolores Jimenez Ulpiano Gonzalez-Larriba Jose Sanchez José Bertran-Alamillo Jordi Mayo Clara de Aguirre Itziar Queralt Cristina Molina Miguel Simonetti Sara Camps Carlos Garcia-Campelo Rosario Massuti Bartomeu Benlloch Susana y Cajal Santiago Taron Miquel Rosell Rafael Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer Journal of Translational Medicine |
| title | Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer |
| title_full | Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer |
| title_fullStr | Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer |
| title_full_unstemmed | Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer |
| title_short | Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer |
| title_sort | detection of egfr mutations with mutation specific antibodies in stage iv non small cell lung cancer |
| url | http://www.translational-medicine.com/content/8/1/135 |
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