Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases
The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) co-carrying multiple carbapenemases is complicating clinical treatment. This study aimed to investigate the global dissemination trends of CRKP strains that co-carry multiple carbapenemases. The CRKP isolate KP424 co-carrying blaNDM-...
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Elsevier
2023-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037023002465 |
| _version_ | 1797384067797221376 |
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| author | Hao Guo Yuye Wu Lirong Li Jianfeng Wang Juan Xu Fang He |
| author_facet | Hao Guo Yuye Wu Lirong Li Jianfeng Wang Juan Xu Fang He |
| author_sort | Hao Guo |
| collection | DOAJ |
| description | The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) co-carrying multiple carbapenemases is complicating clinical treatment. This study aimed to investigate the global dissemination trends of CRKP strains that co-carry multiple carbapenemases. The CRKP isolate KP424 co-carrying blaNDM-1 and blaKPC-2, recovered from a stool specimen, was identified by the NG-Test Carba 5 test, and the genome sequence was further determined by using Nanopore MinION and Illumina NovaSeq 6000 technologies. The genome sequences of the CRKP strains carrying multiple carbapenemase genes were further retrieved from the NCBI GenBank database. Thirteen antimicrobial resistance genes, including blaNDM-1 and blaKPC-2, have been identified in KP424, with blaNDM-1 and blaKPC-2 located on different plasmids. In total, 832 genome sequences of CRKP strains co-carrying two carbapenemase genes were retrieved from the NCBI database. Strains carrying both blaNDM and blaOXA-48-like accounted for 665 (79.9 %) of the total strains, ranking first, and those carrying both blaKPC and blaNDM accounted for 103 (12.4 %), ranking second. The prevalence of CRKP strains co-carrying two carbapenemase genes increased significantly over time, from 0.40 % in 2010 to 9.67 % in 2021. The proportion of strains carrying both blaKPC and blaNDM has also increased, from 0.00 % in 2010 to 4.40 % in 2021. The strains carrying both blaKPC and blaNDM had the highest prevalence (66.7 %, 52/78) in China, while those carrying both blaNDM and blaOXA-48-like had the highest prevalence worldwide. Multiple-carbapenemase producers pose a great threat to public health; further research on the mechanisms underlying multiple carbapenemase gene occurrence is required to prevent their global dissemination. |
| first_indexed | 2024-03-08T21:30:09Z |
| format | Article |
| id | doaj.art-6bed5f6b8ddb4f7489d4ffab56d5312a |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-03-08T21:30:09Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
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| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-6bed5f6b8ddb4f7489d4ffab56d5312a2023-12-21T07:31:47ZengElsevierComputational and Structural Biotechnology Journal2001-03702023-01-012135573563Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemasesHao Guo0Yuye Wu1Lirong Li2Jianfeng Wang3Juan Xu4Fang He5Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang 310014, ChinaDepartment of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, ChinaLaboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang 310014, ChinaDepartment of Respiratory and Critical Care Medicine, Zhejiang Provincial Hospital of Chinese Medicine, Hangzhou, Zhejiang 310003, ChinaSchool of Public Health, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China; Corresponding authors.Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang 310014, China; Corresponding authors.The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) co-carrying multiple carbapenemases is complicating clinical treatment. This study aimed to investigate the global dissemination trends of CRKP strains that co-carry multiple carbapenemases. The CRKP isolate KP424 co-carrying blaNDM-1 and blaKPC-2, recovered from a stool specimen, was identified by the NG-Test Carba 5 test, and the genome sequence was further determined by using Nanopore MinION and Illumina NovaSeq 6000 technologies. The genome sequences of the CRKP strains carrying multiple carbapenemase genes were further retrieved from the NCBI GenBank database. Thirteen antimicrobial resistance genes, including blaNDM-1 and blaKPC-2, have been identified in KP424, with blaNDM-1 and blaKPC-2 located on different plasmids. In total, 832 genome sequences of CRKP strains co-carrying two carbapenemase genes were retrieved from the NCBI database. Strains carrying both blaNDM and blaOXA-48-like accounted for 665 (79.9 %) of the total strains, ranking first, and those carrying both blaKPC and blaNDM accounted for 103 (12.4 %), ranking second. The prevalence of CRKP strains co-carrying two carbapenemase genes increased significantly over time, from 0.40 % in 2010 to 9.67 % in 2021. The proportion of strains carrying both blaKPC and blaNDM has also increased, from 0.00 % in 2010 to 4.40 % in 2021. The strains carrying both blaKPC and blaNDM had the highest prevalence (66.7 %, 52/78) in China, while those carrying both blaNDM and blaOXA-48-like had the highest prevalence worldwide. Multiple-carbapenemase producers pose a great threat to public health; further research on the mechanisms underlying multiple carbapenemase gene occurrence is required to prevent their global dissemination.http://www.sciencedirect.com/science/article/pii/S2001037023002465Klebsiella pneumoniaeCarbapenemaseNDMKPCWhole-genome sequencing |
| spellingShingle | Hao Guo Yuye Wu Lirong Li Jianfeng Wang Juan Xu Fang He Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases Computational and Structural Biotechnology Journal Klebsiella pneumoniae Carbapenemase NDM KPC Whole-genome sequencing |
| title | Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases |
| title_full | Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases |
| title_fullStr | Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases |
| title_full_unstemmed | Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases |
| title_short | Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases |
| title_sort | global emergence of carbapenem resistant klebsiella pneumoniae co carrying multiple carbapenemases |
| topic | Klebsiella pneumoniae Carbapenemase NDM KPC Whole-genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2001037023002465 |
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