Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes
<p>Abstract</p> <p>Based on findings obtained using two-dimensional capillary analyses on tissue cross-sections, diabetes has been shown to be associated with a high risk for microangiopathy and capillary regression in skeletal muscles. We visualized the three-dimensional architect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-11-01
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| Series: | Nutrition & Metabolism |
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| Online Access: | http://www.nutritionandmetabolism.com/content/8/1/77 |
| _version_ | 1811281770829578240 |
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| author | Kondo Hiroyo Fujino Hidemi Murakami Shinichiro Nagatomo Fumiko Roy Roland R Ishihara Akihiko |
| author_facet | Kondo Hiroyo Fujino Hidemi Murakami Shinichiro Nagatomo Fumiko Roy Roland R Ishihara Akihiko |
| author_sort | Kondo Hiroyo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Based on findings obtained using two-dimensional capillary analyses on tissue cross-sections, diabetes has been shown to be associated with a high risk for microangiopathy and capillary regression in skeletal muscles. We visualized the three-dimensional architecture of the capillary networks in the soleus muscle of non-obese Goto-Kakizaki (GK) rats with type 2 diabetes and compared them with those of control Wistar rats to provide novel information, e.g., capillary volume, on the capillary networks. In addition, we examined pro- and anti-angiogenic gene expression levels in the soleus muscle of GK rats using TaqMan probe-based real-time PCR. As expected, plasma glucose levels were higher and insulin levels lower in GK than control rats. The three-dimensional architecture of the capillary networks was regressed and capillary volume was smaller in the soleus muscle of GK compared to control rats. The mRNA expression levels of the pro-angiogenic factors HIF-1α, KDR, Flt-1, ANG-1, and Tie-2 were lower, whereas the level of the anti-angiogenic factor TSP-1 was higher in GK than control rats. These data suggest that a decrease in pro-angiogenic and increase in anti-angiogenic factors may play an important role in type 2 diabetes-induced muscle circulatory complications.</p> |
| first_indexed | 2024-04-13T01:39:37Z |
| format | Article |
| id | doaj.art-6bef45bdae7141babf9561f755b38cb5 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-04-13T01:39:37Z |
| publishDate | 2011-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-6bef45bdae7141babf9561f755b38cb52022-12-22T03:08:13ZengBMCNutrition & Metabolism1743-70752011-11-01817710.1186/1743-7075-8-77Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetesKondo HiroyoFujino HidemiMurakami ShinichiroNagatomo FumikoRoy Roland RIshihara Akihiko<p>Abstract</p> <p>Based on findings obtained using two-dimensional capillary analyses on tissue cross-sections, diabetes has been shown to be associated with a high risk for microangiopathy and capillary regression in skeletal muscles. We visualized the three-dimensional architecture of the capillary networks in the soleus muscle of non-obese Goto-Kakizaki (GK) rats with type 2 diabetes and compared them with those of control Wistar rats to provide novel information, e.g., capillary volume, on the capillary networks. In addition, we examined pro- and anti-angiogenic gene expression levels in the soleus muscle of GK rats using TaqMan probe-based real-time PCR. As expected, plasma glucose levels were higher and insulin levels lower in GK than control rats. The three-dimensional architecture of the capillary networks was regressed and capillary volume was smaller in the soleus muscle of GK compared to control rats. The mRNA expression levels of the pro-angiogenic factors HIF-1α, KDR, Flt-1, ANG-1, and Tie-2 were lower, whereas the level of the anti-angiogenic factor TSP-1 was higher in GK than control rats. These data suggest that a decrease in pro-angiogenic and increase in anti-angiogenic factors may play an important role in type 2 diabetes-induced muscle circulatory complications.</p>http://www.nutritionandmetabolism.com/content/8/1/77angiogenic factorscapillary networkskeletal musclethree-dimensional imagingtype 2 diabetes |
| spellingShingle | Kondo Hiroyo Fujino Hidemi Murakami Shinichiro Nagatomo Fumiko Roy Roland R Ishihara Akihiko Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes Nutrition & Metabolism angiogenic factors capillary network skeletal muscle three-dimensional imaging type 2 diabetes |
| title | Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes |
| title_full | Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes |
| title_fullStr | Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes |
| title_full_unstemmed | Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes |
| title_short | Regressed three-dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non-obese rats with type 2 diabetes |
| title_sort | regressed three dimensional capillary network and inhibited angiogenic factors in the soleus muscle of non obese rats with type 2 diabetes |
| topic | angiogenic factors capillary network skeletal muscle three-dimensional imaging type 2 diabetes |
| url | http://www.nutritionandmetabolism.com/content/8/1/77 |
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