Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines
There is a wealth of resistance genes in the Mexican wild relative of cultivated Solanum, but very few of these species are sexually compatible with cultivated Solanum tuberosum. The most devastating disease of potato is late blight caused by the oomycete Phytophthora infestans (Pi). The wild hexapl...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00699/full |
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| author | Elena Rakosy-Tican Ramona Thieme Janine König Marion Nachtigall Thilo Hammann Tunde-Eva Denes Tunde-Eva Denes Klaudia Kruppa Márta Molnár-Láng |
| author_facet | Elena Rakosy-Tican Ramona Thieme Janine König Marion Nachtigall Thilo Hammann Tunde-Eva Denes Tunde-Eva Denes Klaudia Kruppa Márta Molnár-Láng |
| author_sort | Elena Rakosy-Tican |
| collection | DOAJ |
| description | There is a wealth of resistance genes in the Mexican wild relative of cultivated Solanum, but very few of these species are sexually compatible with cultivated Solanum tuberosum. The most devastating disease of potato is late blight caused by the oomycete Phytophthora infestans (Pi). The wild hexaploid species S. demissum, which it is able to cross with potato, was used to transfer eleven race-specific genes by introgressive hybridization that were subsequently widely used in potato breeding. However, there are now more virulent races of Pi that can overcome all of these genes. The most sustainable strategy for protecting potatoes from late blight is to pyramid or stack broad-spectrum resistance genes into the cultivars. Recently four broad-spectrum genes (Rpi) conferring resistance to Pi were identified and cloned from the sexually incompatible species S. bulbocastanum: Rpi-blb1 (RB), Rpi-blb2, Rpi-blb3, and Rpi-bt1. For this research, a resistant S. bulbocastanum accession was selected carrying the genes Rpi-blb1 and Rpi-blb3 together with race-specific R3a and R3b genes. This accession was previously used to produce a large number of somatic hybrids (SHs) with five commercial potato cultivars using protoplast electrofusion. In this study, three SHs with cv. ‘Delikat’ were selected and backcross generations (i.e., BC1 and BC2) were obtained using cvs. ‘Baltica’, ‘Quarta’, ‘Romanze’, and ‘Sarpo Mira’. Their assessment using gene-specific markers demonstrates that these genes are present in the SHs and their BC progenies. We identified plants carrying all four genes that were resistant to foliage blight in greenhouse and field trials. Functionality of the genes was shown by using agro-infiltration with the effectors of corresponding Avr genes. For a number of hybrids and BC clones yield and tuber number were not significantly different from that of the parent cultivar ‘Delikat’ in field trials. The evaluation of agronomic traits of selected BC2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool. |
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| last_indexed | 2024-12-23T10:08:08Z |
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| spelling | doaj.art-6bf153826c8d493986f222af443ee75a2022-12-21T17:51:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00699501103Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding LinesElena Rakosy-Tican0Ramona Thieme1Janine König2Marion Nachtigall3Thilo Hammann4Tunde-Eva Denes5Tunde-Eva Denes6Klaudia Kruppa7Márta Molnár-Láng8Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, RomaniaFederal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, GermanyFederal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg, GermanyFederal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, GermanyFederal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, GermanyPlant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, RomaniaBiological Research Centre, Jibou, RomaniaCentre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, HungaryCentre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, HungaryThere is a wealth of resistance genes in the Mexican wild relative of cultivated Solanum, but very few of these species are sexually compatible with cultivated Solanum tuberosum. The most devastating disease of potato is late blight caused by the oomycete Phytophthora infestans (Pi). The wild hexaploid species S. demissum, which it is able to cross with potato, was used to transfer eleven race-specific genes by introgressive hybridization that were subsequently widely used in potato breeding. However, there are now more virulent races of Pi that can overcome all of these genes. The most sustainable strategy for protecting potatoes from late blight is to pyramid or stack broad-spectrum resistance genes into the cultivars. Recently four broad-spectrum genes (Rpi) conferring resistance to Pi were identified and cloned from the sexually incompatible species S. bulbocastanum: Rpi-blb1 (RB), Rpi-blb2, Rpi-blb3, and Rpi-bt1. For this research, a resistant S. bulbocastanum accession was selected carrying the genes Rpi-blb1 and Rpi-blb3 together with race-specific R3a and R3b genes. This accession was previously used to produce a large number of somatic hybrids (SHs) with five commercial potato cultivars using protoplast electrofusion. In this study, three SHs with cv. ‘Delikat’ were selected and backcross generations (i.e., BC1 and BC2) were obtained using cvs. ‘Baltica’, ‘Quarta’, ‘Romanze’, and ‘Sarpo Mira’. Their assessment using gene-specific markers demonstrates that these genes are present in the SHs and their BC progenies. We identified plants carrying all four genes that were resistant to foliage blight in greenhouse and field trials. Functionality of the genes was shown by using agro-infiltration with the effectors of corresponding Avr genes. For a number of hybrids and BC clones yield and tuber number were not significantly different from that of the parent cultivar ‘Delikat’ in field trials. The evaluation of agronomic traits of selected BC2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.https://www.frontiersin.org/article/10.3389/fpls.2020.00699/fulleffectorsdetached leaf assayintrogression breedinglate blight field resistancegene specific markersprocessing qualities |
| spellingShingle | Elena Rakosy-Tican Ramona Thieme Janine König Marion Nachtigall Thilo Hammann Tunde-Eva Denes Tunde-Eva Denes Klaudia Kruppa Márta Molnár-Láng Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines Frontiers in Plant Science effectors detached leaf assay introgression breeding late blight field resistance gene specific markers processing qualities |
| title | Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines |
| title_full | Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines |
| title_fullStr | Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines |
| title_full_unstemmed | Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines |
| title_short | Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines |
| title_sort | introgression of two broad spectrum late blight resistance genes rpi blb1 and rpi blb3 from solanum bulbocastanum dun plus race specific r genes into potato pre breeding lines |
| topic | effectors detached leaf assay introgression breeding late blight field resistance gene specific markers processing qualities |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00699/full |
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