Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China
Abstract Background Human adenoviruse (HAdV) is a major pathogen of paediatric respiratory tract infections (RTIs). Mutation or recombination of HAdV genes may cause changes in its pathogenicity and transmission. We described the epidemiology and genotypic diversity of HAdV in hospitalized children...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-09-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-021-01661-6 |
| _version_ | 1818438395021492224 |
|---|---|
| author | Yiman Huang Chao Wang Fenlian Ma Qiong Guo Lihong Yao Aijun Chen Xiaoyi Luo Lishu Zheng |
| author_facet | Yiman Huang Chao Wang Fenlian Ma Qiong Guo Lihong Yao Aijun Chen Xiaoyi Luo Lishu Zheng |
| author_sort | Yiman Huang |
| collection | DOAJ |
| description | Abstract Background Human adenoviruse (HAdV) is a major pathogen of paediatric respiratory tract infections (RTIs). Mutation or recombination of HAdV genes may cause changes in its pathogenicity and transmission. We described the epidemiology and genotypic diversity of HAdV in hospitalized children with RTIs in Beijing, China. Methods Nasopharyngeal aspirates were collected from hospitalized children with RTIs from April 2018 to March 2019. HAdVs were detected by a quantitative real-time PCR, and the hexon gene was used for phylogenetic analysis. Results Among 1572 samples, 90 (5.72%) were HAdV-positive. The HAdV detection rate was highest in November and July. Among HAdV-positive children, 61.11% (55/90) were co-infected with other respiratory viruses, the most common of which were human respiratory syncytial virus and human rhinovirus. The main diagnosis was bronchopneumonia, most patient have cough and fever. Children with a high viral load were more likely to have a high fever (P = 0.041) and elevated WBC count (P = 0.000). Of 55 HAdV-positive specimens, HAdV-B (63.64%), HAdV-C (27.27%), and HAdV-E (9.09%) were main epidemic species. Phylogenetic analysis indicated that hexon sequences of three samples were on the same branch with the recombinant HAdV strain (CBJ113), which was circulating in Beijing since 2016. Conclusion The HAdV-B3 and HAdV-B7 are the main epidemic strains in Beijing, and the recombinant HAdV-C strain CBJ113 has formed an epidemic trend. |
| first_indexed | 2024-12-14T17:39:53Z |
| format | Article |
| id | doaj.art-6bf29e762c274f91a07d814795e25732 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-14T17:39:53Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-6bf29e762c274f91a07d814795e257322022-12-21T22:52:53ZengBMCVirology Journal1743-422X2021-09-011811910.1186/s12985-021-01661-6Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, ChinaYiman Huang0Chao Wang1Fenlian Ma2Qiong Guo3Lihong Yao4Aijun Chen5Xiaoyi Luo6Lishu Zheng7NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCNHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDCAbstract Background Human adenoviruse (HAdV) is a major pathogen of paediatric respiratory tract infections (RTIs). Mutation or recombination of HAdV genes may cause changes in its pathogenicity and transmission. We described the epidemiology and genotypic diversity of HAdV in hospitalized children with RTIs in Beijing, China. Methods Nasopharyngeal aspirates were collected from hospitalized children with RTIs from April 2018 to March 2019. HAdVs were detected by a quantitative real-time PCR, and the hexon gene was used for phylogenetic analysis. Results Among 1572 samples, 90 (5.72%) were HAdV-positive. The HAdV detection rate was highest in November and July. Among HAdV-positive children, 61.11% (55/90) were co-infected with other respiratory viruses, the most common of which were human respiratory syncytial virus and human rhinovirus. The main diagnosis was bronchopneumonia, most patient have cough and fever. Children with a high viral load were more likely to have a high fever (P = 0.041) and elevated WBC count (P = 0.000). Of 55 HAdV-positive specimens, HAdV-B (63.64%), HAdV-C (27.27%), and HAdV-E (9.09%) were main epidemic species. Phylogenetic analysis indicated that hexon sequences of three samples were on the same branch with the recombinant HAdV strain (CBJ113), which was circulating in Beijing since 2016. Conclusion The HAdV-B3 and HAdV-B7 are the main epidemic strains in Beijing, and the recombinant HAdV-C strain CBJ113 has formed an epidemic trend.https://doi.org/10.1186/s12985-021-01661-6Human adenovirusRespiratory tract infectionEpidemiologyGenetic diversity |
| spellingShingle | Yiman Huang Chao Wang Fenlian Ma Qiong Guo Lihong Yao Aijun Chen Xiaoyi Luo Lishu Zheng Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China Virology Journal Human adenovirus Respiratory tract infection Epidemiology Genetic diversity |
| title | Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China |
| title_full | Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China |
| title_fullStr | Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China |
| title_full_unstemmed | Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China |
| title_short | Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China |
| title_sort | human adenoviruses in paediatric patients with respiratory tract infections in beijing china |
| topic | Human adenovirus Respiratory tract infection Epidemiology Genetic diversity |
| url | https://doi.org/10.1186/s12985-021-01661-6 |
| work_keys_str_mv | AT yimanhuang humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT chaowang humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT fenlianma humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT qiongguo humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT lihongyao humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT aijunchen humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT xiaoyiluo humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina AT lishuzheng humanadenovirusesinpaediatricpatientswithrespiratorytractinfectionsinbeijingchina |