Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins.
Domain swapping mechanism is a specialised mode of oligomerization of proteins in which part of a protein is exchanged in a non-covalent manner between constituent subunits. This mechanism is highly affected by several physiological conditions. Here, we present a detailed analysis ofthe effect of pH...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4456399?pdf=render |
| _version_ | 1798043831512334336 |
|---|---|
| author | Prashant Shingate Jim Warwicker Ramanathan Sowdhamini |
| author_facet | Prashant Shingate Jim Warwicker Ramanathan Sowdhamini |
| author_sort | Prashant Shingate |
| collection | DOAJ |
| description | Domain swapping mechanism is a specialised mode of oligomerization of proteins in which part of a protein is exchanged in a non-covalent manner between constituent subunits. This mechanism is highly affected by several physiological conditions. Here, we present a detailed analysis ofthe effect of pH on different regions of the domain swapped oligomer by considering examples which are known to be sensitive to pH in transiting from monomeric to domain-swapped dimeric form. The energetic calculations were performed using a specialized method which considers changes in pH and subsequent changes in the interactions between subunits. This analysis provides definitive hints about the pH-dependence switch from monomer to domain-swapped oligomer and the steps that may be involved in the swapping mechanism. |
| first_indexed | 2024-04-11T22:54:29Z |
| format | Article |
| id | doaj.art-6bf2e533cb9447e192325baf8b675a0b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:54:29Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6bf2e533cb9447e192325baf8b675a0b2022-12-22T03:58:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012771610.1371/journal.pone.0127716Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins.Prashant ShingateJim WarwickerRamanathan SowdhaminiDomain swapping mechanism is a specialised mode of oligomerization of proteins in which part of a protein is exchanged in a non-covalent manner between constituent subunits. This mechanism is highly affected by several physiological conditions. Here, we present a detailed analysis ofthe effect of pH on different regions of the domain swapped oligomer by considering examples which are known to be sensitive to pH in transiting from monomeric to domain-swapped dimeric form. The energetic calculations were performed using a specialized method which considers changes in pH and subsequent changes in the interactions between subunits. This analysis provides definitive hints about the pH-dependence switch from monomer to domain-swapped oligomer and the steps that may be involved in the swapping mechanism.http://europepmc.org/articles/PMC4456399?pdf=render |
| spellingShingle | Prashant Shingate Jim Warwicker Ramanathan Sowdhamini Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. PLoS ONE |
| title | Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. |
| title_full | Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. |
| title_fullStr | Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. |
| title_full_unstemmed | Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. |
| title_short | Energetic Calculations to Decipher pH-Dependent Oligomerization and Domain Swapping of Proteins. |
| title_sort | energetic calculations to decipher ph dependent oligomerization and domain swapping of proteins |
| url | http://europepmc.org/articles/PMC4456399?pdf=render |
| work_keys_str_mv | AT prashantshingate energeticcalculationstodecipherphdependentoligomerizationanddomainswappingofproteins AT jimwarwicker energeticcalculationstodecipherphdependentoligomerizationanddomainswappingofproteins AT ramanathansowdhamini energeticcalculationstodecipherphdependentoligomerizationanddomainswappingofproteins |