Rooting and acclimatization of transgenic papaya plants var. Maradol roja

The disease caused by Papaya ringspot virus is the most important in papaya worldwide. The use of biotechnological techniques, as auxiliary tools, has facilitated the genetic improvement in papaya. Nevertheless, this species has a lot of constraints, mainly during rooting and acclimatization phases....

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Main Authors: Maylin Cruz, Ana L. Darías, Dariel Cabrera, Amado Pérez, Mileidy Cruz-Martín, Tatiana Pichardo, Rafael Gómez-Kosky, Orelvis Portal
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2008-01-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/333
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author Maylin Cruz
Ana L. Darías
Dariel Cabrera
Amado Pérez
Mileidy Cruz-Martín
Tatiana Pichardo
Rafael Gómez-Kosky
Orelvis Portal
author_facet Maylin Cruz
Ana L. Darías
Dariel Cabrera
Amado Pérez
Mileidy Cruz-Martín
Tatiana Pichardo
Rafael Gómez-Kosky
Orelvis Portal
author_sort Maylin Cruz
collection DOAJ
description The disease caused by Papaya ringspot virus is the most important in papaya worldwide. The use of biotechnological techniques, as auxiliary tools, has facilitated the genetic improvement in papaya. Nevertheless, this species has a lot of constraints, mainly during rooting and acclimatization phases. For that reason we developed the present work. The in vitro and ex vitro rooting was evaluated. Culture media with different concentrations of indol-3-butiric acid hormone were used in the in vitro rooting. The influence of Trichoderma harzianum bioproduct in the acclimatization of plants was also studied. The in vitro rooting of transgenic plants was achieved by applying 2 mg.l-1 of indol-3-butiric acid in the culture medium. The ex vitro rooting with high percentages of plants survival was also obtained. The applications of T. harzianum bioproduct on the substrate, previous to the plantation, demonstrated its stimulating effect. Key words: Carica papaya, ex vitro, in vitro, Trichoderma harzianum
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spelling doaj.art-6bf3439234d84cbf90fc762e41f351f52022-12-22T01:06:31ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472008-01-0181307Rooting and acclimatization of transgenic papaya plants var. Maradol rojaMaylin CruzAna L. DaríasDariel CabreraAmado PérezMileidy Cruz-MartínTatiana PichardoRafael Gómez-KoskyOrelvis PortalThe disease caused by Papaya ringspot virus is the most important in papaya worldwide. The use of biotechnological techniques, as auxiliary tools, has facilitated the genetic improvement in papaya. Nevertheless, this species has a lot of constraints, mainly during rooting and acclimatization phases. For that reason we developed the present work. The in vitro and ex vitro rooting was evaluated. Culture media with different concentrations of indol-3-butiric acid hormone were used in the in vitro rooting. The influence of Trichoderma harzianum bioproduct in the acclimatization of plants was also studied. The in vitro rooting of transgenic plants was achieved by applying 2 mg.l-1 of indol-3-butiric acid in the culture medium. The ex vitro rooting with high percentages of plants survival was also obtained. The applications of T. harzianum bioproduct on the substrate, previous to the plantation, demonstrated its stimulating effect. Key words: Carica papaya, ex vitro, in vitro, Trichoderma harzianumhttps://revista.ibp.co.cu/index.php/BV/article/view/333
spellingShingle Maylin Cruz
Ana L. Darías
Dariel Cabrera
Amado Pérez
Mileidy Cruz-Martín
Tatiana Pichardo
Rafael Gómez-Kosky
Orelvis Portal
Rooting and acclimatization of transgenic papaya plants var. Maradol roja
Biotecnología Vegetal
title Rooting and acclimatization of transgenic papaya plants var. Maradol roja
title_full Rooting and acclimatization of transgenic papaya plants var. Maradol roja
title_fullStr Rooting and acclimatization of transgenic papaya plants var. Maradol roja
title_full_unstemmed Rooting and acclimatization of transgenic papaya plants var. Maradol roja
title_short Rooting and acclimatization of transgenic papaya plants var. Maradol roja
title_sort rooting and acclimatization of transgenic papaya plants var maradol roja
url https://revista.ibp.co.cu/index.php/BV/article/view/333
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AT amadoperez rootingandacclimatizationoftransgenicpapayaplantsvarmaradolroja
AT mileidycruzmartin rootingandacclimatizationoftransgenicpapayaplantsvarmaradolroja
AT tatianapichardo rootingandacclimatizationoftransgenicpapayaplantsvarmaradolroja
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